法

Eight hierarchical clustering methods including single linkage method, complete linkage method, median linkage method, centroid method, unweighted pair-group average method, Wards method, flexible-beta method and weighted pair-group average method were combined with three sampling strategies, random, preferred and deviation sampling to develop 24-core collections.

川最短距离法、最长距离法、中间距离法、重心法、不加权类平均法、离差平方和法、可变法和加权配对算术平均法等8种系统聚类方法，结合随机取样法、优先取样法和变异度取样法等3种取样方法，发展了24个水稻核心种质库。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法；钙测定用间接法也较好，其次是偶氮砷Ⅲ法；磷测定使用磷钼酸紫外法结果较好；血糖测定用己糖激酶法结果较好；尿素测定用脲酶紫外速率法结果较好；肌酐用酶法结果较好；白蛋白测定用溴甲酚绿法较好；胆红素测定用酶法结果好，其次是钒酸氧化法；碱性磷酸酶测定使用AMP缓冲液结果较好；淀粉酶测定酶法底物法和碘比色法变异系数均较大，前者稍小，合格率后者稍高，各实验室可根据自己的条件加以选用。

Geothermochronology based on mineral closure temperatures and geochronological methods such as U-Pb, Sm-Nd, Rb-Sr, Lu-Hf, Re-Os, 40Ar/39Ar, FT,/He, TIMS U-series, cosmogenetic nuclide (including 14C, etc.) and 3H/3He for young groundwater dating is reviewed in this paper.

简要介绍了基于矿物封闭温度的地质热年代学，并对多种地质年代学方法，包括U-Pb法、Sm-Nd法、Rb-Sr法、Lu-Hf法、Re-Os法、40Ar/39Ar法、裂变径迹测年、/He法、TIMS铀系法、宇宙成因核素定年（包括14C法等）和年轻地下水测年（如3H/3He法等）进行了综合评述。

The precision, linearity, anti -interference ability, recovery rate were compared among the 4 methods according to the standard method of NCCLS, and relativity between each of the 4 methods and modified diazotate J - G was calculated. Results

结果改良化学氧化法、钒酸氧化法、胆红素氧化酶法的批内精密度均小于3.0％；4种方法的回收率均在95.0％～105.0％之间；KHC3法、改良化学氧化法、钒酸氧化法和胆红素氧化酶法的线性范围分别是：0～424μmol／L、0-675μmol／L、0-629μmol／L、0-575μmol／L；胆红素氧化酶法和改良化学氧化法均有较强的抗干扰能力，和改良J-G法的测定结果有高度一致性。

Physical therapy using various palpating skills to stimulate specific portions or Acupuncture points on the body for treating disease、especially pain problems.

推拿是用各种手法在患者肢体的部位或穴位上进行刺激的一种物理疗法，常用手法有推法、拿法、按法、摩法、擦法、掍法、揉法、摇法、扳法、拍击法、搓法、抖法、捻法、背法、踩跷法、拔伸法等。

The methods of extracting and refining tellurium presently were mainly soda roasting,autoclaving in general basic solution,sulfating roasting,oxidative leaching in acid solution, solvent extraction ,emulsion liquid membrane,microbial method,electrorefining,vacuum distillation and zone melting etc.

目前分离提纯碲的方法主要有苏打粉焙烧法、碱性高压浸出法、硫酸化焙烧法、氧化酸浸法、萃取法、液膜法、微生物法、电解精炼法、真空蒸馏法、区熔精炼法等，其中电解精炼法得到的碲的纯度达到99.99%，真空蒸馏碲的纯度达到99.999%，区熔精炼碲的纯度可达99.999995%，本文综述了以上分离提纯碲的方法。

Along with operations of the turning, sharpping, drilling, milling and grinding, some new methods, technologies and processings such as overlap workpieces, improved cutting tool formes, workpiece charmfer, adjustment of cutting parameters and others to restrain and decrease burrs in size are developed, which have noticeable theoretical significance and wide practical value in the precision cutting, FMS and automatic machining.

结合车削、刨削、钻削、铣削和磨削等加工，开发出了斜置工件法，双主偏角刀具切削法、切削用量选择法、刀具几何参数调整法、工件叠加切削法、工件终端部材料脆化法、工件终端部倒角法、挡板切削法、刀具结构改进法和切削方式选择法等十数种抑制或减小切削毛刺的技术、工艺和方法，在精密加工、柔性制造系统和其它自动化加工中有较大的实际应用价值。

This course is an attempt to give the students the basic idea and method of forest management in order to prepare the management plan; Place of forest management in forestry; Content of forest management; Guide principles of forest management; Sustained working; Silvicultural system; Forest yield; Increment; Volume increment; Quality increment; Price increment; Total price increment; Forest maturity; Indicating percent; Productive stage; Physical rotation; Technical rotation; Rotation of maximum volume yield; Highest forest rent rotation; Financial rotation; Cutting cycle of selection system; Normal forest; Normal distribution of age classes; Normal arrangement of stands; Normal increment; Normal growing stock; Normal yield; Improvement of actual forest; Reserve forest; Reserve fund; Planning and plan of forest management; Forest regulation; Area allotment; Volume allotting method; Volume frame work; Area-volume combined frame work; Growing stock; Utilization percent method; Difference method; Pure age-class; Growth method; Control method; Forest subdivision; Forest surveying; Forest survey; Certainty of future policy of forest; Selection of species; Decision of silvicultural system; Decision of rotation; Regulation of future yield; Preparation of management plan; Working the plan; Control and revision of management

本课程之目的为使学生了解森林经营之基本观念及方法，以期编订经营计划，课程包括森林经营学在林学上之地位、森林经营学之内容、森林经营之指导原则、保续作业、森林作业法、森林收获、生长、材积生长、形质生长、腾贵生长、总价格生长、林木成熟期、指率、生产期、自然轮伐期、工艺轮伐期、材积收获最多轮伐期、森林纯益最大之轮伐期、财政轮伐期、择伐林之回归期、法正林、法正龄级分配、法正林分排列、法正生长、法正蓄积、法正伐采额、现时林之改良、预备林、预备金、森林经营上之规划及计划、收获预定、面积配分法、材积配分法、材积平分法、折衷平分法、蓄积法、利用率法、较差法、纯粹龄级法、生长量法、稽核法、森林区划、森林测量、森林调查、将来森林经营方针之确定、树种之选定、作业法之决定、轮伐期之判定、将来收获之预定、经营管理计划之编订、计划之执行、经营管理计划之稽核与检订。

CITY RIDE \ 环法自行车赛 Spain's Alberto Contador, in the overall leader's yellow jersey, rode past the Arc de Triomphe during the 21st stage of the Tour de France in Paris Sunday.

上周日，西班牙车手康塔德身穿黄色领骑衫，在环法自行车赛第21赛段中经过巴黎凯旋门。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。