油菜

22 Of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%,95.4%,100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.

（1）30对小麦EST-SSR引物中，有29，28和26对引物分别在玉米、油菜和大豆中有扩增产物，可扩增率分别为96.7%，93.3%和86.7%；其中分别有12对和9对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有4对引物在4种作物中的扩增产物均显示多态性。

The cotyledon handle from Brassica napu L. cultivars Huayou No 5 and Huayou No 6 were transformed by Agrobacterium-mediated method.

为了使该基因在油菜种子中特异表达，采用油菜种子特异启动子BcNA1，构建了植物表达载体pBIBE。

The plumpness ratio is the bestindicator of full rapeseed.(5) The equivalent diameter is used to estimate the weight of rapeseed, andestablish the special and common model. In the overall, the correlation coefficient ofpredicted and measured values was over 0.99. The accuracy is more than 95%.

5以等价直径估测油菜种子重量，分别建立各品种的专用模型和面向白菜型、甘蓝型油菜的通用模型，两类模型在整体预测方式下的预测值与实测值间的相关系数均在0.99以上，预测精度可达到95％以上。

In this paper, the research progresses on the effects of endogenous phytohormones to the development in rapeseed were reviewed.

笔者主要从油菜生长发育的这几个方面对油菜内源激素的研究作一概述。

This study on the efficiency of transformation transgenic rapeseed provides a molecular basis for the further research..

PCR检测法为计算油菜的转化效率以及转基因油菜的进一步研究提供了分子基础。

Because of application of mixed nitrate rare-earth, rape growth was promoted and the output of rapeseed was enhanced.

由于硝酸混合稀土改善了油菜钾的营养状况，因而促进了油菜的生长，提高油菜籽的产量。

KCR and HCD are erucic acid synthesis genes. In order to reveal the relationship between the synthesis of erusic acid and the quantitative expression of its functional genes, and to reveal the theoretic base of the breeding for high erucic acid content, the expression of KCR and HCD had been analyzed kinetically and quantitatively by using regular reverse transcription polymerase chain reaction and the internal standards of QuantumRNA18S during the growing period of high erucic acid content lines of Brassica Juncea.

研究应用常规RT-PCR(Reverse transcription polymerase chain reaction)的技术和QuantumRNA18S内标对芥菜型高芥酸油菜生育期内芥酸合成基因KCR、HCD基因的表达进行量化分析，了解芥酸的合成与功能基因量化表达的关系，为芥菜型油菜的高芥酸品种的选育提供理论依据。

Under different stresses, we tested the expression of citrate synthase gene in rapeseed leaf by using semi-quantitative PCR. The expression of citrate synthase gene had no obvious change in stresses of salt, dark, high illumination, while was increased at different time in treatments of water logging, drought, IAA, and 6-BA. Interestingly the effect of ABA was contrary to that of IAA. In the treatment of sclerotium blight, the expression of citrate synthase gene was depressed. There was a saddle curve of citrate synthase gene expression in the treatment of gibberellin.

对油菜幼苗进行植物生长调节物质、高温和低温、强光照和弱光照、盐、菌核病、干旱和水渍等处理，采用半定量PCR法对油菜叶片柠檬酸合酶基因的表达模式进行检测，发现在盐胁迫、暗光和强光的处理下，柠檬酸合酶基因的表达基本没有变化；在水渍、干旱、IAA和6-BA胁迫下，其表达有所升高，但出现峰值的时间不同，ABA对表达模式的影响与IAA相反，感染菌核病后其表达降低；对GA3的应答呈鞍型。

Advances in studies on formation and inheritance of seed capsule color,and molecular marker and breeding for yellow seed capsule in rapeseed were reviewed.

综述了油菜种皮颜色的形成、种皮色泽的遗传、黄籽性状的分子标记和黄籽甘蓝型油菜选育等方面的研究进展。

These results suggested that silique wall chlorophyll content was a typical quantitative trait controlled by many minor-effect genes and influenced by environment. Molecular markers linked with these QTLs will help to understand the genetic basis of silique wall chlorophyll content and related photosynthetic traits.

本研究认为油菜角果叶绿素含量属于典型的数量性状，受环境影响很大，与这些QTL紧密相关的分子标记可以对油菜角果叶绿素及其相关光合性状的研究奠定基础。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。