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The medium composition and some fermentation parameters were studied by cross test and single factor experiments. The optimal conditions are as flows: Tryptone 0.76 %, Bean powder 1.0 %,initial pH 8.0, temperature 28 ℃, inoculation quantity 8 % and loading volume 30 %. Under optimal conditions, the maximum yield of biomass and microbial lipids reached 29.8 g/L and 11.7 g/L, respectively, which was 2.59 times and 2.05 times of those cultured in the original medium, after 9 days fed-batch co-fermentation in shake-flask.

采用单因子和正交实验对培养基组成和发酵条件进行了优化,结果表明,诱变菌在温度28 ℃,pH 8.0,接种量8 %,装液量30 %,蛋白胨0.76 %和豆粕1 %,分批补糖的最优发酵条件下发酵9 d,生物量和菌体油脂积累量分别达29.8 g/L和11.7 g/L,较出发菌提高了2.59倍和2.05倍,同时原料糖利用率达到99.4 %。

The optimal nitrogen source is tryptone, the optimal carbon source is glucose, the optimal condition for lipase producing are inoculation for 1.5%, initial pH7.0, clutivated aerobically at 28℃with an aeration rate of 200rpm for 48 hours.

作者从成都及周边地区汽车修理厂,机油厂、植物油厂、餐厅、菜市场等富含油脂的土壤中,用溴甲酚紫平板筛选得到80余株产脂肪酶的菌株,其中大部分酶活在10—20u/ml范围内。

Light revitalizing mist refreshes skin throughout the day while helping keep skin clean and unclogged. Help control oil and keep your skin revitalized and moisturized all day long!

除了维持脸部清洁,避免脏东西沾污,阻碍尘埃附著於脸上,保持毛孔呼吸顺畅,亦有平衡油脂,补充水分的保湿功能,使肌肤一整天下来都无后顾之忧。

Efficacy:rapidly remove the blackheads and excessive sebum which block pore,restrain and smooth acnes,eliminate the roo of acnes and prevent acnes coming into being again,unblock and shrink pore,improve the mini-circulation of skin,removing acnes without trace,combined with high-concentration essence ingredients,deep condition skin and prevent secretion of flourish greases,balance the PH of skin,let your skin is fresh and unpinguid,upgrade the smooth of skin,skin present unpinguid undry healthy soft and ideal state.

快速去除堵塞毛孔的角栓和多余皮脂,抑制及抚平暗疮痘痘,消除引发暗疮痘痘根源并预防青春痘再次形成,疏通、收细毛孔,改善皮肤微循环,祛痘不留印痕;结合高浓度精华成份,深层调理肌肤及抑制旺盛的油脂分泌,平衡酸碱肌肤,时刻清爽不油腻,提升肌肤的光滑度,肌肤呈现不油不干的健康柔美理想状态。

The invention uses low cost grease which are mainly waste resources such as the unedible Jatropha curcas seed oil, the waster oil in the restaurant industry and the acid value oil, etc. as raw materials, thereby enlarging the material sources; because the Rhizopus chinensis CCTCC M201021 full cell lipase which is extremely low in cost is used as the catalyst, the problem of high price catalyst is effectively resolved; the toxic action of the alcohol on the biological catalyst is resolved by the batching fed-batch the short chain alcohol, thereby improving the conversion rate of the short chain alcohol.

本发明以廉价油脂为原料,主要是非可食用性的麻疯籽油以及餐饮业废油、酸价油等废物资源,扩大了原料的来源;采用价格极其低廉的华根霉全细胞脂肪酶作为催化剂,有效地解决了催化剂价格高昂的问题;分批流加短链醇以解决醇对生物催化剂的毒害作用,提高短链醇转化率。

Of soap containing extra unsaponified fat.

含有过多的未皂化油脂

Efficacy:deep clean skin,remove the dirts and leftover which block pore and caused blackheads and comedones easily,help to reduce the excessive greases of face,tighten thick pore step by step,let pore restore to pure and unpolluted state,it is untight after cleansing,let skin recur healthy and elastic luster.

功效:深层清洁肌肤,清除堵塞毛孔及易导致黑头、粉刺的污垢和残留物,有助减少面部多余油脂,逐步收敛粗大毛孔,让毛孔回复纯净无污染状态,洗后不紧绷,令你的肌肤重现健康有弹性的光泽。

Efficacy:removing the deep dirts which hided in skin and easily absorb the excessive greases,settle the problem of oilness,the red megranate,miracle fruit of detoxify,totally eliminate the harmful substances caused skin to be darkness,sallowness and tireness,skin is fresh and untight after washing,skin is active and transparent with vigor.

功效:能够祛除隐藏在肌肤深层的污垢,轻松吸附多余油脂,解决油光问题;具有&排毒圣果&之称的红石榴将导致肌肤暗沉、萎黄、疲惫的有害物质全部排除,洗后清爽不紧绷,肌肤鲜活剔透,充满生机。

Any cat-like, carnivorous mammal of the subfamily Viverrinae that secrets a yellowish, unctuous substance with a strong musk-like odor.

一种像猫一样的食肉哺乳动物,系灵猫的一个亚种,会分泌一种有浓烈麝香气味的黄色油脂状物质。

A brown, fatty, waxlike substance that forms on dead animal tissues in response to moisture.

尸蜡,脂蜡,尸油对潮湿起反应而形成于动物尸体组织的一个棕色蜡状油脂物质

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