没有降低的

Next,we transfected the HOXD13 expression vector,luciferase reporter driven by EPHA7 promoter and renilla luciferase reporter into mouse C3H10T1/2 cell. Thirty six hours after transfection,relative luciferase activity was tested by luminometer.

结果显示，与野生型HOXD13蛋白相比，p.Q317R突变体对EPHA7启动子的转录激活作用明显降低，下降至野生型的13%，而p.1314L突变体对EPHA7启动子的转录激活作用也降低，但降低程度没有p.Q317R明显，为野生型的63%。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Interstitial pneumonitis was reduced with polyvalent IVIG in older studies but not in the more recent ones, nor in studies assessing CMV-IVIG.

在早先的研究中，多价免疫球蛋白降低了间质性肺炎的风险，但是最近的研究没有得出这一结果，而且没有研究评价巨细胞病毒免疫球蛋白能否降低间质性肺炎。

Supplementation SP or probiotics can increase the laying rate of hen,hen day egg production and mean egg weight,and decrease feed/egg ratio,whenas the addition of SS can not.③The addition of either SP or SS can increase liver IDⅠactivity and serum T_3,and decrease T_4 of hen.Especially when SP was supplemented,the liver IDⅠactivity and serum T_3 of hen increased and serum T_4 of hen decreased as supplemental So level increased.

添加SP或益生菌均可提高蛋鸡产蛋率、日产蛋量和平均蛋重，降低料蛋比；③添加SS或SP均能提高蛋鸡肝IDⅠ酶的活性和血清T_3水平，降低T_4水平，特别是添加SP时，随着硒添加水平的升高，蛋鸡肝脏IDⅠ酶的活性和血清T_3水平升高，血清T_4水平则降低；而添加益生菌对蛋鸡肝IDⅠ酶的活性没有显著影响，却能显著提高血清T_3水平，而降低T_4水平。

In 300mg/kg JSS treatment, the yolk color was 6 degree too. While added 500mg/kg JMH into the basal diet, the egg yolk color was up to 9 degree. Supplement of oxytetracycline + JMH or chlortetracy dine + JMH in the basal diet, the egg yolk color was up to 8 and 6 degree respectively and decreased one and three color unit respectively than that of JMH treatment. Added oxytetracyclimne + JSS or chlortetracycline + JSS, the egg yolk color was up to 8 and 6degree respectively. When added arsanilic acid + JSS, the egg yolk color was up to 7 degree and improved one color unit than that of JSS treatment. When added arsanilic acid +JMH to basal diet, the egg yolk color was up to 8 degree and decreased one color unit than that of JMH treatment.

饲料中添加300mg/kg的金闪闪，蛋黄颜色也是6级，没有产生增色效果，添加500mg/kg的加美黄，可使蛋黄颜色达到9级，提高3个比色单位，土霉素与加美黄配合使用，蛋黄颜色为8级，比加美黄组降低1级，金霉素与加美黄配合使用蛋黄颜色为6级，比加美黄组降低3级，土霉素与金闪闪配合使用可使蛋黄颜色升到8级，比金闪闪组提高2级，金霉素与金闪闪配合使用蛋黄颜色仍为6级，阿散酸与金闪闪配合使用，蛋黄颜色为7级，比金闪闪组提高1级，阿散酸与加美黄配合使用蛋黄颜色为8级，比加美黄组降低1级。

In the presence of Aβ42, carnosine significantly decreased glutamate release, which was further reduced by clobenpropit and thioperamide.

我们发现Aβ42对胞外谷氨酸含量几乎没有影响，肌肽能显著降低胞外谷氨酸含量，而H_3受体拮抗剂clobenpropit和thioperamide能进一步降低由肌肽引起的胞外谷氨酸含量的降低。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

The results show that:First, concentration of hydrogen peroxide have the most obvious influence to oil yield and carbon black yield,with the increase of concentration of hydrogen peroxide the oil yield first increase then decrease,when concentration of hydrogen peroxide is 8.19% or so,the oil yield reach to the biggest value, and with the increase of concentration of hydrogen peroxide carbon black yield decrese;Then,reaction time have more obvious influence to oil yield and carbon black yield,oil yield increase but carbon black yield decrease with the increase of reaction time;Thirdly,reaction tempreture have some influence to oil yield and carbon black yield,samely,oil yield increase but carbon black yield decrease with the increase of reaction tempreture;Finally,reaction pressure does almost no influence on oil yield and carbon black yield.

研究结果表明：过氧化氢质量浓度对产油率和炭黑产率的影响最为显著，产油率随过氧化氢质量浓度的提高先提高后降低，在过氧化氢质量浓度为8.19%左右时达到最大值，炭黑产率随过氧化氢质量浓度的提高而降低；反应时间对产油率和炭黑产率的影响也比较显著，产油率随反应时间的增加而提高，炭黑产率随反应时间的增加而降低；反应温度对产油率和炭黑产率也有一定的影响，产油率随反应温度的提高而相应地提高，炭黑产率随反应温度的提高而降低；在本文实验范围内，反应压力对产油率和炭黑产率基本上没有影响。

In that case to re-treat, the chance to have a sustained response to the new treatment is a bit low.

如果患者根本就没有应答，没有病毒载量的降低，也根本就没有丙肝病毒的降低。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新