没有报道的

Fatty acid desaturase gene was cloned from genomic DNA and cDNA of Euglena gracilis through PCR with primer designed acroding to the reported equences.This gene had an open reading frame of 1266bp that encodes 421 amino acids.

根据已报道的Δ8-脂肪酸脱氢酶基因设计引物，分别从小眼虫藻基因组DNA和cDNA中扩增得到该基因片段，序列分析结果表明，结构基因长1266bp，编码421氨基酸，而且没有内含子，这是国内外首次报道。

Imputation methods were used to estimate outcome ariances where not reported.

用填补的方法分析没有报道的结果的差异。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Titin is recently known as the largest protein which exists in the stiated muscle sarcomere and is dynamic both in biomechanics properties and biochemical functions. Four possible disease-associated mutations located in three exons(3,14,49) of titin gene have been identified in Japanese DCM patients in 2002. But we did nothing about the creative titin and DCM.

目的：肌联蛋白是由单基因编码的最大蛋白，普遍存在于心肌和骨骼肌，被称为第三肌丝，具有复杂的生物力学性质和生物化学功能。2002年日本研究人员报道了肌联蛋白基因第3、14、49外显子的4个基因突变可能与扩张性心肌病发病相关，而我国没有相关的研究报道。

When anthrone react with aryl aldehyde in the presence of pyridine by using xylene as the solvent,besides 10 arylmethylene anthrone derivatives we found other oxygenated anthrone which have not been reported.

发现其生成物中除含有目标化合物 1 0 -芳香基甲叉基蒽酮外，还含有部分文献中没有报道的蒽酮氧化物，经物理方法确定其为蒽醌。

It was reported that two passenger ferries had been sunk but no details of casualties were given.

据报道有两艘渡轮沉没但没有伤亡的细节报道。

The deeply alarming report was compiled after months of secret, insider leaks - with the help of a couple of the Chinese factory workers and reveals a prison-like regime of misery, faint intimidation, squalid conditions, strict fines for the tiniest mistakes, dreadful food, illegal lack of insurance, 80-hour work weeks, 'Big Brother'-esque monitoring, hard wooden stools without backrests for the duration of their 12-hour day, and frequently stolen/unpaid wages which sometimes amount to as much as 20% knocked-off their already inadequate wages.

这个令人深深为之震惊的报道是在一对中国工人夫妇的帮助下，经过数月秘密内幕的整理完成的。该报道揭示了工厂里像牢狱般严苛的工作制度，工人们受到的威逼胁迫，肮脏的住宿环境，因为细微错误的严格罚款，可怕的食物，没有合法的保险，每周工作80小时，专治的监控。工人们要坐在没有靠背的硬板凳上工作12个小时，经常被偷和被拖欠的工资有时候占到他们已经微薄收入的20%。

I have many friends and a large family here and I'm taking advantage of the opportunity to see everyone, have Argentine cookouts and getting some rest.

我只是希望，他的伤没有报道的那么严重，因为我真的无法想象，在缺少姚的情况下怎么样打整个赛季，这将是非常困难的。

For metabolites of strain SIPI-800, three active compounds were found, named as SIPI-800-1, 2 and 3 respectively, moreover SIPI-800-1 was new compound showing immunosuppressant activity and moderate anti- anaerobic activity; 2 and 3 were isoflavones with estrogenic activity, attracting activity to fungal zoospores, inhibiting alcohol dehydrogenase and aldehyde dehydrogenase I activities.

对SIPI-800菌株的代谢产物研究，分离出3个活性化合物，分别命名为：SIPI-800-1、2和3，其中1化合物为文献没有报道的新化合物，具有免疫抑制活性（1ug/ml浓度抑制NK细胞88.9%的活性）和弱的抗厌氧菌活性；2和3为异黄酮化合物，分别为Daidzein和Genisten的7甲基化衍生物，具有雌激素活性、强吸引真菌游动孢子、抑制乙醇或醛脱氢酶等活性。

SINEs insertion can be stably delivered to descendants, thereby, SINEs can provide the reliable evolutionary information, and used as molecular markers for phylogeny events within 50Mys, such as the levels of family and order.Cyprinidae, the largest fish family, comprises approximately 210 recognized genera and 2010 species.

鲤科鱼类是世界上现生鱼类中最大的一个科，约有210属2010个种，但有关鲤科鱼类中SINEs的研究却很少，目前相关的报道仅限于斑马鱼基因组内；而有关利用SINEs插入位点信息进行鲤科鱼类系统进化的研究更没有报道。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐