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ACTH〓 at concentration of 10〓-10〓 had no significant influence on the saturation curve, Scatchard plot and Hill plot of 〓-DAGO binding to rat brain membrane.

〓在〓范围内对〓的饱和曲线,Scatchard图形及Hi11图形没有明显的影响;但在较高浓度时可以完全取代〓与其受体的结合,〓为37.1±21.8μmol/L,其抑制μ配体〓结合的强度与δ受体的配体DPDPE和κ受体的配体U50488H大致相当,只是稍弱一些,分别是它们的1/8和1/4。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白,本实验以美洲商陆夏季叶片为材料,通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增,对PAPⅡ进行了基因克隆、序列分析,结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9%,然后将该基因构建到原核、真核表达载体上,分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达,在两个表达系统中均获得了有活性的PAPⅡ表达蛋白,体外生物测定表明表达的蛋白均具有抑制病毒的活性,PAPⅡ基因在酵母中还没有表达的报道,这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The results showed that though apoHb could be reconstituted with all FePs, it showed different functions. Binding of apoHb with hemin can enhance the heterolytic cleavage of O-O bond of H〓O〓, but inhibiting the cleavage when FeTCPP and FeTPPS〓 were used instead of hemin. The binding of apoHb with FeTTMAPP showed negligible influence on the cleavage of O-O bond in H〓O〓.

结果表明,虽然具有阴离子基团的FeTCPP和FeTPPS〓能够在天然血红蛋白中辅基位置的附近与脱辅基血红蛋白结合,但是由于结构上与天然辅基的差别,使得血红素蛋白中相应的氨基酸残基不能起到有效的作用,因此不但不能提高反而抑制了其过氧化物酶活性;而对于具有阳离子基团的FeTTMAPP,脱辅基血红蛋白对其活性没有明显的影响。

As similar defects are not exhibited by mice that experience systemic inhibition of NF-B, we postulated that the morphogenetic defects in IKK--deficient mice are not caused by reduced NF-B activity but instead are due to failed epidermal differentiation that disrupts proper epidermal–mesodermal interactions.

因为类似的缺陷没有出现在经历了全身NF-B抑制的小鼠,作者推断IKK缺失小鼠的形态发生缺陷不是由于NF-B活性降低引起而是因为可以破裂专属表皮-中胚层相互作用的表皮分化失败引起。

It may be laid down, I think, that the more archaic the code, the fuller and the minuter is its penal legislation.

至于刑法,则并没有同样的使它贫乏的理由,因此,纵使我们不应冒昧地宣称在国家的幼年时代总是一个无法抑制的强暴时期,我们仍旧应该懂得为什么刑法和民法的现代关系竟在古代法典中颠倒过来。

All of these had shown that the induced resistance could be transmitted.Oligosaccharides had not shown any direct activity against Puccinia striifomis in vitro. This suggests that Oligosaccharides can elicit resistance to diseases of wheat, and are different from common pesticides.

不同浓度(25ppm、50ppm、100ppm)的5号提取物的体外抑菌试验表明,低聚糖对条锈菌没有明显的抑制作用,说明低聚糖不具有直接杀菌或抑菌作用,使小麦产生抗锈性的原因在于诱发寄主植物的抗病潜能,使植物本身产生对病原菌的抗性,这与普通杀菌剂显然不同。

Meanwhile, the water bloom was seldom occurred in this lake which may be attributed to the low illumination due to high sediment resuspension caused by fish and boat activity in this lake.

惠州西湖的游植物密度平均为5958.63×10~4Cells/L,约为杭州西湖的1/3左右,没有明显的水华发生,这可能是由于西湖鱼类和游船引起的悬浮物浓度较高,使光照强度降低,从而抑制浮游植物的生长。

Results showed that low contents of Cd has promotion effect on Oryza Sativa, but high contents of Cd has not obvious restrain on it. The total trend of distribution of Cd in Oryza Sativa is root>stem>shell>rice. The relation between Cd contents in rice and total Cd in soil as well as availability Cd in soil has been investigated. The result showed that both the total Cd and the availability Cd had notably correlation with content of Cd to rice, and the critical point were CaCl2-Cd=0.71mg/kg, DTPA-Cd=1.34 mg/kg, EDTA-Cd=0.76 mg/kg, NH4OAc-Cd=0.77 mg/kg.

研究表明:低浓度的镉对水稻生长稍有促进作用,高浓度的镉对其生长并没有明显的抑制作用;镉在水稻植株中总的分布趋势为根>茎>稻壳>糙米;用4种有效态提取剂(0.1 mol/L CaCl2、DTPA、0.05 mol/L EDTA和1 mol/L NH4OAc)浸提出的有效态镉的含量和土壤中镉总量与水稻糙米中镉含量均达到显著相关水平,并确定了它们的临界值分别为CaCl2-Cd=0.71 mg/kg, DTPA-Cd=1.34 mg/kg, EDTA-Cd=0.76 mg/kg, NH4OAc-Cd=0.77 mg/kg。

The samples were partitioned into leaf, stem and sheath, stalk and chaff of spike, and grain, then microwaved for 4 min, and dried in an oven at 60℃.

施氮对氮素积累量的杂种优势没有显著的影响,但对氮素含量的杂种优势有显著的抑制效应。

Although the principles of Arminius were solemnly condemned in the great Calvinist Synod held at Dordrecht, or Dort, in 1618-19, and the "Remonstrant heresy" was rigorously suppressed during the lifetime of Maurice of Orange, nevertheless the Leyden professor had given to ultra-Calvinism a blow from which it never recovered.

虽然原则亚米纽斯谴责了庄严的伟大加尔文主义主教举行了多德雷赫特,或者多特,在1618年至1619年,和&忠告异端&是在严格抑制的寿命莫里斯的橙色,但在莱顿教授给予超加尔文主义的打击来自它从来没有收回。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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