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The columna soil study showed that strain NBT had the ablity of potassiumuptake ,making losed potassium from the soil inoculated with the bacterium wasdecreased by

通过土柱试验发现,硅酸盐细菌NBT菌株还具有保钾功能,供试土壤接菌处理后的土壤滤液中流失的钾比对照减少35.5-56.5%,经方差分析,差异达显著水平;另外,硅酸盐细菌NBT菌株与农药的相容性试验表明,无论在大田农药施用浓度和残留浓度内的丁草胺、绿黄隆、三唑酮粉、多菌灵、马拉硫磷等农药对NBT菌株没有抑制作用;乐果、甲基1605在大田施用浓度下,对NBT菌株有一定抑制作用,而在残留浓度下,对NBT菌株无明显抑制作用。

The result indicated that the olive extract showed better inhibition to the selected bacteria, but proved weak antimicrobial effect to microzyme and ineffective against mildew.

结果表明,橄榄乙醇提取物对细菌有较好的抑制作用,对酵母菌的抑制效果较弱,对霉菌几乎没有抑制作用。

S extract had an inhibition effect against Sphaerotheca fuligenea,The inhibition rate was 100% when extract concentration was 20mg/mL and the inhibition rate got to 67.66% even at the concentration of 2.5mg/mL. The extract of E. humifusa had different preventive effects against four strains of S. fuliginea from four districts in Shanghai. The preventive effects on the Qibao and Baoshan were better than those of Nanhui and Qingpu. The microorganism inhibition experiment by filter papers indicated that the extract of E. humifusa had higher inhibition effect against bacteria than on pathogenic fungus. Diameters of the zone of inhibition of salmonellas, Echerichia coli and Staphylococcus aureus were 15.77mm, 12.27mm and 10.53mm respectively. The extract of E. humifusa had a certain effect against Colletotrichum lagenarium, Botrytis cinerea, Rhizoctomia solan and Pyricularia oryza but no effect against Helminthosporium maydis and Gibberella zea. The 4 cation exchange resins and ethanol deposition were used to isolate the active fractions from supernate after alkali deposition.

提取液对黄瓜白粉病菌体外分生孢子萌发抑制实验表明地锦提取液对黄瓜白粉病菌分生孢子的萌发有很好的抑制作用,抑制率随着地锦提取液浓度的增大而增高,当浓度为20mg/mL时,抑制率达到100%,即使在浓度为2.5mg/mL时,抑制率也达到了67.66%;室内盆栽苗期预防性实验表明了地锦提取液对上海4个不同地区的黄瓜白粉病菌均具有不同程度的抑制效果,对七宝和宝山地区的菌株抑制效果优于南汇和青浦地区的菌株;用滤纸片法测定了地锦提取液的抑菌谱,实验结果表明地锦提取液对供试的4种植物病原真菌:黄瓜炭疽病菌、黄瓜灰霉病菌、水稻纹枯病菌和水稻稻瘟病菌均具有一定的抑制效果,而对玉米小斑病菌和小麦赤霉病菌没有抑制效果,对供试的3种人类致病细菌沙门氏菌、大肠杆菌和金黄色葡萄球菌均具有很好抑菌效果,抑菌圈直径分别为:15.77mm, 12.27mm和10.53mm。

It is Concluded that the mode of action of pyrimidine benzylamine differs from the other typically commercial ALS inhibitors, such as sulfonylurea and pyrimidine salicylic acid herbicides. Its herbicidal activity is activated by the metabolic processes in the plants.

离体条件下,化合物104-4 IC_(50)>100 mg/L,而除草剂双草醚IC_(50)值为10~(-5)~10~(-4)mg/L,说明化合物104-4对离体ALS没有抑制作用;活体条件下,化合物104-4对ALS有一定的抑制作用,且随着处理时间的延长,酶活力降低,对ALS的抑制作用增加。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体,所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合,其特征在于所述抗体a是IgG1同种型,b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1,c当与没有所述抗体的这种测定比较时,其在5nM的浓度上,在包含0.5%的热灭活胎牛血清的培养基中,使用3T3细胞的细胞磷酸化测定中,抑制至少80%的IGF-IR磷酸化,所述3T3细胞提供400,000-600,000分子IGF-IR/细胞,和d当与没有所述抗体的这种测定相比,在包含0.5%的热灭活胎牛血清的培养基中,使用3T3的细胞磷酸化测定中,其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性,所述3T3提供400,000-600,000分子IGF-IR/细胞。

On the inhibition to mycelial growth, the 95% ethanol extract had significantly inhibition to P. ultimum, F. oxysporum f. sp cucumber, R. solani and B. cinerea, and at the top treatment concentration of 0.002 g/mL, the inhibition rate reached to 60.32% 58.50%, 55.89% and 55.17%, respectively; But the 95% ethanol extract almost had no inhibition to S. rolfsii, its rate was just 17.19% at 0.002 g/mL.③On the inhibition to sprouting, the 95% ethanol extract had significantly inhibition to B. cinerea, R. solani, F. oxysporum f. sp cucumber and P. ultimum, and at the top treatment concentration of 0.002 g/mL, the inhibition rate reached to 95.24%, 90.48%, 89.47% and 85.71%, respectively; and the rate would exceed 40% to four fungi mentioned above when their concentration was not lower to 0.005g/mL. The 95% ethanol extracthad infirm inhibition to S. rolfsii, its rate was just 43.48% at 0.002 g/mL.

在抑制菌丝生长方面,鱼腥草95%醇提物对豌豆根腐病菌、黄瓜枯萎病菌、马铃薯立枯丝核病菌和大白菜灰霉病菌均有良好的抑制作用,在最高处理浓度为0.002 g/mL时抑制率分别达到60.32%、58.50%、55.89%和55.17%;对辣椒白绢病菌几乎没有抑制作用,在最高处理浓度为0.002 g/mL时抑制率仅为17.19%;③在抑制孢子萌发方面,鱼腥草95%醇提物对大白菜灰霉病菌、马铃薯立枯丝核病菌、黄瓜枯萎病菌和豌豆根腐病菌抑制效果明显,在最高处理浓度为0.002 g/mL时的抑制率分别达到95.24%、90.48%、89.47%和85.71%,且在处理浓度不低于0.000 5 g/mL时对以上4种真菌孢子的抑制率超过40%;对辣椒白绢病菌抑制作用较弱,在最高处理浓度为0.002 g/mL时仅为43.48%。

The results showed that the three disparate treated substances have no visible antibacterial effect to E. coli , but a obvious effect to Staphylococcus aureus with inhibition of about 10 bacteriostasis units; have dissimilar effects to 10 kinds of fungi among the total kinds, and have a good inhibitory effect to Cytospora.coccodesHughes with fungistasis rate above 65%. After the gel filtration and activity detection, 50mM ammonium acetate was screened out as the more suitable eluents, the crudes were separated into strikingly unlike parts with obviously different antimicrobial activity.

结果表明,大蜡螟抗菌物质对大肠杆菌没有明显的抑制作用,而对金黄葡萄球菌抑制作用明显,抑菌活性单位在10左右;对10种植物病原真菌均有不同的抑菌活性,其中对苹果树皮腐烂病菌的抑制率达到65%以上;经过凝胶过滤分离,及对分离组分的抑菌活性测定,筛选出较合适的洗脱液为50mM乙酸铵,各分离组分抑菌活性差异明显,粗提物被分成了两个差异显著的部分。

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本实验利用Prep Cell纯化玉米萌芽谷粒和大豆子叶中的beta-淀粉酶,分别将两种不同物种的beta-淀粉酶与胰脂解酶预先反应后,再加入脂解酶反应液中观察活性的变化,结果发现脂解酶的活性未如预期,没有抑制的效果产生,将甘薯beta-淀粉酶以同样方法与胰脂解酶作用,结果同上述两种beta淀粉酶依旧没有抑制的现象。

For metabolites of strain SIPI-800, three active compounds were found, named as SIPI-800-1, 2 and 3 respectively, moreover SIPI-800-1 was new compound showing immunosuppressant activity and moderate anti- anaerobic activity; 2 and 3 were isoflavones with estrogenic activity, attracting activity to fungal zoospores, inhibiting alcohol dehydrogenase and aldehyde dehydrogenase I activities.

对SIPI-800菌株的代谢产物研究,分离出3个活性化合物,分别命名为:SIPI-800-1、2和3,其中1化合物为文献没有报道的新化合物,具有免疫抑制活性(1ug/ml浓度抑制NK细胞88.9%的活性)和弱的抗厌氧菌活性;2和3为异黄酮化合物,分别为Daidzein和Genisten的7甲基化衍生物,具有雌激素活性、强吸引真菌游动孢子、抑制乙醇或醛脱氢酶等活性。

MCP could inhibit the respiration of climacteric fruits such as Starking and Fuji apple and Feicheng peach in different degree, and this effect was not be able to convert by exogenous ethylene. On the contrary, the effect of 1-MCP on the non-climacteric fruit was not ascertainment: 1-MCP inhibited the respiration of strawberry stored at 20℃ during the whole storage time, while when stored at 0℃, 1-MCP didn't inhibit the strawberry respiration.

1-MCP能够不同程度的抑制跃变型果实富士、红星苹果和肥城桃的呼吸作用,而且这种作用可以不为外源乙烯所拮抗,但对非跃变型果实草莓的呼吸作用的效果则不确定:1-MCP抑制了20℃下草莓的呼吸速率,但并没有抑制0℃下贮藏草莓的呼吸作用。

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