沉默的

RNA interference represents a common biological phenomenon of double-stranded RNA mediated post-transcriptional gene silencing, which lies in altitude plants and animals. The mechanism of this process is described as that: the dsRNA in cells are cut into double-stranded small interfering RNA. By joining with an effector complex termed RISC (RNA-induced silencing complex), siRNA binds to the cellular RNA with homologous sequences, and contributes to the degradation of the corresponding mRNA, the inhibition of specific gene expression.

RNAi技术是双链RNA(double strand RNA，dsRNA)介导的序列特异性的转录后基因沉默，广泛存在于高等植物和动物中，其机制是细胞内的dsRNA被Dicer剪切成双链的小分子干涉RNA (small interfering RNA，siRNA)与RNA诱导的基因沉默复合物结合，降解与之互补的靶mRNA，特异性抑制靶基因表达。

And the soldiers, guardian, PK killed when stars are not杀杀of Kazakhstan, in addition to killing stars to flicker and then followed by a row of skills (this time the role of 5800's life played out on the full), followed by three kinds of this job is not simply rely on into my body, and魔道PK when you remember he is a gram Therefore, and魔道playing, you just made 42 of the cis and Dr. skills and drugs out of the ball, but you should never silly place to enlarge the station to retribution, and his other close千万千万千万body, his silence on you running quickly, and time is too silent, and on the skills to beat him shun fat, remember, and his other close千万千万千万, operation of the dual differential scattering approach also meters.

和战士，守护，杀星PK时都是随便杀杀的哈，除了杀星能闪烁然后接下来一排技能(这个时候5800的生命的作用就完全发挥出来了)，接下来这3种职业是根本靠不进我身的，和魔道PK时，记住他是克你的，所以和魔道打时，你只要用42的顺发技能和毒和招出来的小球，千万别傻乎乎的站原地放大地报应，千万千万千万别和他靠近身，他沉默你了就赶紧跑，等沉默时间过了，就顺发技能打他，记住千万千万千万别和他近身，操作差的偶也米办法撒。

In the second place, both good logic and sound common sense demand that the silence of Ecclesiasticus concerning certain points of doctrine be not regarded as a positive denial of them, unless it can be clearly and conclusively shown that such a silence must be so construed.

第二，良好的逻辑和健全的常识要求保持沉默Ecclesiasticus的某些点的理论是不被视为一个积极的拒绝他们，除非它可以清楚地和决定性地表明，这种沉默必须如此解释。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

A mute appeal; a silent curse; best grief is tongueless- Emily Dickinson; the words stopped at her lips unsounded; unspoken grief; choking exasperation and wordless shame- Thomas Wolfe.

无语的请求；无语的咒骂；最深的悲伤是沉默——艾米丽·狄金森；话到了她的嘴边又沉默了；无语的悲痛；憋闷的愤怒和无语的羞愧——托马斯·乌尔夫。

As Tannen (1985) suggests, silence in itself is not necessarily a sign of powerlessness, just as volubility in itself is not necessarily a sign of domination.

有些时候，沉默会被视为某人的一种权利的暗示，或者控制他人的示语，(引用：Jaworski， 19993)， Tannen 1985启示：沉默本身不是一种必然的无权力的象征，就像健谈本身也不是一种必然的主宰的象征。

Open, create, and save audio files in any of the supported formats (can also save any portion of a loaded file to disk as a new file); Display audio data waveform (Zoom Full, Zoom In, Zoom Out, Zoom Vertical); Play audio files or any portion of the files; Record audio data from a microphone or any other available input device; Edit audio files visually (Cut, Copy, Delete Selection, Delete Silence, Paste, Paste From File, Mix, Mix From File); Apply various effects (Amplify, Compressor, Delay, Equalize, Fade In and Fade Out, Flanger, Invert, Normalize, Phaser, Reverb, Reverse, Silence, Shrink, Stretch, Vibrato, etc.); Apply different filters to any selected portion of audio files; Supports all major audio file formats such as uncompressed WAV; compressed WAV; MP3, MP2; Ogg; WMA; CDA; AVI; AIFF; AU; G.721, G.723, G.726; VOX; RAW.

音频编辑与魔术你可以：开放、创造、救任何音频文件格式的支持(也可以节省一部分的任何档案装作为一个新的磁盘档案)；数据显示音频波形(变焦满变焦，变焦，变焦垂直)；发挥音频文件或任何部分档案；音频记录数据可从麦克风或其他输入设备；视音频编辑档案(减、拷贝、选择删除，删除沉默，糊，糊，从立案、组合，从组合档案)；运用各种效果(放大、压缩、延迟、均衡、而在淡出褪色、对接、转化、规范化、移相、混响、逆向、沉默、退缩、舒展、颤音等)；采用不同过滤任何选定部分音像档案；支持所有主要的音频文件格式如无压缩波；压缩波； MP3及MP2等； Ogg；医协；综合；中AVI； aiff；凹； G.721、g.723、压缩；氧化钒；生吃。

We are not enemys but friends,we must not be enemys,though passion miss strange,but must not break our brothership of faction,the misses called memory will somewhen we again touched,and I'm sure they will be, by the betterr angels in our nature.

一位网友写的很好：生命太短暂，我们不能用她来愤世嫉俗。过去我们愤怒的太多，是该学会沉默了，当然长时间的沉默后应该是爆发。手挽手，看着那一座座高楼在绚烂的烟花中倒塌。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

A highly conserved sequence of 210 bp of AtNHX1 gene that encodes a Na+/H+ antiport protein was isolated from A.thaliana,the 210 bp fragment was amplified as RNAi target site and used in construction of RNAi vector,and was inserted into pHANNIBAL as reverted repeat with an intron.The expressional box was linked into the vector of pART27,NPTⅡgene is the screening marker in this vector.

利用RNAi研究植物基因功能，可选择靶基因的一段单一序列使基因家族中的单个成员沉默；也可以选择某一基因家族的多个成员具有的一段同源性很高的保守序列，设计针对这一序列的dsRNA表达载体使多个基因同时沉默，RNAi成为功能基因组学研究的重要手段。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。