沉默

In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing.

然后把均一化之后mRNA反转录得到cDNA，再与基因沉默载体pTRV重组，最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中，从而建立起破色期番茄果实均一化cDNA沉默文库。

It distinguishes between the avant-gardism,Modernism and Postmodernism, and points out the "silent" feature of the avant-garde literature, highlights Hassans uniqueness among the postmodern theorists.

主要阐述先锋派、现代主义和后现代主义的区别，先锋派文学的"沉默"特征，"沉默"文学内在含义等，突显哈桑在后现代理论家中的独树一帜。

We are the country of Ahab, Bartleby and the Confidence Man: Our choice is silence, fraud or lunatic visions.

我们国家充斥着那种贪得无厌的人、沉默的人以及骗子：我们不是沉默，就是欺诈，或者是疯狂。

Day in silence and angrily center 一天一天 past Finally one day,the fish adds water said that,"heard in this world has plants thething to be extremely beautiful, is called the snow He chastely hasno time, beautiful is varied If I can see the snow, also this haslived not in vain I must go seek snow."

水沉默。日子在沉默与愤然中一天一天地过去。终于有一天，鱼对水说：听说这世上有种东西极美，叫做雪。他纯洁无暇，美丽多姿。若我能看到雪，也不枉此生了。

RNA interference represents a common biological phenomenon of double-stranded RNA mediated post-transcriptional gene silencing, which lies in altitude plants and animals. The mechanism of this process is described as that: the dsRNA in cells are cut into double-stranded small interfering RNA. By joining with an effector complex termed RISC (RNA-induced silencing complex), siRNA binds to the cellular RNA with homologous sequences, and contributes to the degradation of the corresponding mRNA, the inhibition of specific gene expression.

RNAi技术是双链RNA(double strand RNA，dsRNA)介导的序列特异性的转录后基因沉默，广泛存在于高等植物和动物中，其机制是细胞内的dsRNA被Dicer剪切成双链的小分子干涉RNA (small interfering RNA，siRNA)与RNA诱导的基因沉默复合物结合，降解与之互补的靶mRNA，特异性抑制靶基因表达。

DNA methylation is usually associated with triggering histone deacetylation, chromatin condensation, and gene silencing.

DNA甲基化通常与组蛋白脱乙酰化、染色质浓缩和基因沉默相关，染色质浓缩，和基因沉默。

I am not decrying silent courage, but I think that the silence has been over valued. It may be, to some extent, the cause for the alarming increase in mental breakdowns.

当然，我不是贬低沉默的勇气，而只是觉得这种沉默的价值被夸大了，而且从某种程度上说，可能正是由于这种沉默才使精神崩溃的案例惊人地增加了。

Chapter Two explores Ruths silence before American whites, the imposed silence by racism and the way she finds out to break her silence and put forward her self-assertion, namely writing her own stories.

第二章分析了美国华裔女儿露丝面对美国白人所处的沉默地位，沉默背后潜藏的种族歧视和露丝最后通过写自己的故事来打破她所处的沉默地位。

Mandolin boat Swing in the moon Shepherd and Mary I understand, life is today See you next week Sunny Tuesday You were my story I understand, life is too funny I don't know why I sigh Love was to entertain I don't know, follow me Till the moonlight fades away Fades away, woo The silence' on fire, and you stay with me Never know, never speak We shut the door behind Counting matches How warm is here You are my glory I wish to say, you were to save me I don't know why I sigh Love was to entertain I don't know what to do Kiss my heart and sing me a song woo, woo The silence' on fire and you stay with me La la la I still…I think we can sail away We can sail away if we have the sea Lonely star And I wish you were here to stay with me Never stay I sing a song of how you sang with me Let it be Cannot recall the boat beneath the sun La la la And I can say you made me silent

曼陀林船摆在月球谢泼德和玛丽我的理解是，今天的生活看到你下周阳光星期二你是我的故事我的理解是，生活太可笑我不知道为什么我的叹息爱情是受理我不知道，跟我来直到月光消失了消失了，雨沉默'着火，你留在我永远不知道，从来没有讲我们关上了门背后计数比赛如何在这里热烈你是我的荣耀我想说，你能救我我不知道为什么我的叹息爱情是受理我不知道该怎么办吻我的心和我唱的歌曲宇，宇沉默'着火，你留在我拉啦啦我还是。。。我觉得我们可以航行距离我们可以航行距离如果我们有海孤独之星我祝你在这里留在我切勿留我唱的一首歌你唱我的让我们不记得船底下的太阳拉啦啦我可以告诉你们使我保持沉默

This article focused on the mechanisms, characteristics, and associated assays used to identify these proteins, and the prospects of those suppressors were also discussed.

论述了主要沉默抑制因子的机制、特点和相关确认沉默抑制因子实验，并对沉默抑制因子的研究前景进行了展望。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。