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The crude protein was obtained by extracting the leaves of Blechnum orientale with PBS and precipitating with (NH4)2SO4. The crude protein was purified by Sephadex G-100 filtration after treatment through active carbon column.

乌毛蕨的叶片经PBS抽提和硫酸铵沉淀,得到的粗蛋白经活性碳柱处理后,在分子筛层析柱Sephadex G-100上纯化得到一种凝集素(Blechnum Orientale Lectin,简称BOL)。

There are five classes of stainless steel, namely Martensitic, Ferritic, Austenitic, Duplex and Precipitation Hardening

有5个类别的不锈钢,即马氏体,铁素体,奥氏体,双相和沉淀硬化

Calcitonin is a hormone, composed of 32 amino acid residues. The main function of CT is to suppress osteoclastic resorption of bones followed by decreases in serum Ca level in mammals.

降钙素是由32个氨基酸残基组成的一种多肽类激素,其主要生理功能是参与体内的钙磷代谢,通过抑制破骨细胞的活性,促进钙在骨中的沉淀,从而降低血钙含量。

Fine carbides precipitated from ferrite in 16-6 Cr-Mo, 16-2.5-6 Cr-Ni-Mo, and 16-5-6 Cr-Ni-Mo steels in the early stages of ageing are metastable, during prolonged heating they redissolve and are gradually replaced by χ-phase. Some residual carbides serve as the nuclei of the growing χ-phase. The structure of quenched 16-15-6 Cr-Ni-Mo steel is austenitic, during subsequent heating only M_6C and M_(23)C_6 mixed carbides are precipitated.

在16-6Cr-Mo、16-2.5-6及16-5-6Cr-Ni-Mo不锈钢中,从铁素体内沉淀出来的细小碳化物是一种过渡组织,在x相的成核与长大过程中,它们将不断地进行溶解。16-15-6 Cr-Ni-Mo不锈钢属奥氏体型,在不同温度进行固溶处理,奥氏体都很稳定,在随后的恒温处理过程中,只有碳化物从奥氏体内析出。

Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后,用免疫荧光的方法检测β-catenin的表达和在细胞上定位的变化;用半定量RT-PCR检测β-catenin mRNA表达的变化;用质粒转染的方法检测荧光素酶报告基因Tcf转录的情况;用半定量RT-PCR检测Wnt/β-catenin通路下游基因COX-2,cyclinD1, c-fos, c-Jun mRNA表达的情况;用免疫共沉淀的方法检测β-catenin和E-Cadherin的结合情况。

The N gene protein was expressed in the CHO cells proved by IFA. The N gene was transcripted as proved with RT-PCR. CHO/CDV-N cell strain was constructed, which provided evidence for research of CDV serumal detection and gene vaccine.

然后通过磷酸钙共沉淀法转染CHO-K1细胞,通过潮霉素筛选得到阳性克隆,间接免疫荧光实验鉴定N基因在CHO细胞中的表达,并用RT-PCR方法从转录水平证实N基因在CHO-K1细胞中的表达,最终建立了CHO/ CDV-N细胞株,为犬瘟热病毒的血清学检测和基因疫苗的研制奠定了基础。

Methods:Determination method for diazepam and vitamin B1 were changed from coefficient rate and silicotungstic acid precipitation to HPLC and UV respectively.

地西泮由系数倍率法改为HPLC法;谷维素仍用UV法,溶剂由正庚烷改为硫酸乙醇;维生素B1由硅钨酸沉淀法改为UV法。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷(5-Aza-2'-dc,以下简称Aza)及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后,用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况,用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况,并用逆转录聚合酶链反应检测ING1bmRNA表达。

Pure natural whitening: Includes the rich whitening factor, contains the strong effect , the application raises fine practices purely natural can maintain long-time AA2G vitamin C carbohydrate Gansu, Xiong Guosu, suppresses the melanin growth and can permeate the flesh first floor cell fast, tracing precipitates the stubborn melanin, scatters effectively abreast in row except the melanin accumulation, suppresses wheat Ranim to form, desalinates the black spot, has no time naturally the flesh by to outside the clean whitening, the long time maintains sharp white, sends the skin lubrication condition tightly, perfectly clear fair.

纯美净白:富含丰富的净白因子,蕴含强效,应用精纯持久 AA2G 维他命 C 醣甘、熊果素,抑制黑色素并能快速渗入肌肤底层细胞,追踪沉淀顽固黑色素,有效驱散并排除黑色素聚集,抑制麦拉宁形成,淡化色斑,令肌肤由里至外自然无暇净白,持久亮白,紧致润滑,清透白皙。

The main reasons were that the algae and organism in the water decreased the ability of removing pollutants by coagulations, the algae which grown again in the open sedimentation tank weakened the removal effect of algae by coagulation; because of the accumulation of algae and algae toxin in the filtration tank, the concentration of algae toxin in the effluent increased; by the function of fluid chlorine, the algae and organisms after the filtration will release microalgae toxin and produce a lot of byproduct such as microalgae toxin, sobomyl and so on, meanwhile, the chlorination produce considerable halide and halo-acetic acid and other disinfection byproduct.

主要表现在藻类和溶解性有机物的存在降低了混凝去除污染物的能力;藻类在露天平流沉淀池中再生长,抵消了混凝除藻效果;藻类和藻毒素在滤池中积累,导致滤后藻毒素升高;穿透滤池的藻类和有机物在液氯作用下,释放微囊藻毒素、土臭素和二甲基异冰片等胞内物质,同时生成数量可观的卤代烃和卤乙酸等消毒副产物。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?