水解蛋白质的

The first step in degradation of the protein is considered to be hydrolysis, induced by hydrolytic enzymes.

蛋白质降解的第一步是由水解酶引起的水解作用。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

In this paper, the study status of enhancing the protein emulsification by modification methods such as deamidation, proteolysis, glycosylation, phosphorylation and graft copolymerization were introduced, the development direction of the preparation of new type protein emulsifier was also pointed out,it provided a reference for the future study.

综述了脱酰胺作用、蛋白水解作用、糖基化作用、磷酸化作用、接枝改性等蛋白质改性技术对提升蛋白质乳化性的研究现状，展望了制备新型蛋白质乳化剂的发展方向，为今后蛋白质乳化剂的研究提供参考。

The products that we have been developed are: intermediate include Protoporphyrin,, protein peptone, plant peptone; Flavor material include hydrolyzed vegetable protein, meat flavor protein powder; food material include compound amino acid powder, soybean peptide,soybean polypeptide,blood peptide, collagen polypeptide, fish protein powder and hydrolyzed animal protein; protein powder include hydrolyzed soybean protein, hemoglobin,hemocrystallin and food-grade whey protein.

目前的已经开发的产品有医药中间体：原卟啉、卟啉铁、蛋白胨、植物蛋白胨；调味品原料：水解植物蛋白粉、肉味蛋白粉；食品原料：复合氨基酸、甘氨酸、大豆缩氨酸、大豆多肽、血肽、胶原多肽、鱼蛋白粉、水解动物蛋白粉和蛋白质粉系列产品：饮料专用型蛋白质粉、血红蛋白质粉、肉制品专用型蛋白质粉。进口的产品有乳清蛋白粉。

The plastein reaction is considered to involve the formation of polypeptides by a reversal of the usual peptide bond hydrolysis to produce "resynthesized" protein or polypeptides from a hydrolysate of protein.

类蛋白反应通常认为是酶水解的逆反应，是指在一定条件下，通过蛋白酶的催化作用将高浓度的蛋白质水解物与蛋白酶一起加热处理，生成一种类似高分子蛋白质物质的反应。

An S_N2 mechanism with water as the nucleophile appears to be the most likely candidate.We reported for the first time the hydrolysis reaction mechanism of amide in high temperature water,and found a scientistic base for the hydrolysis possibility of amide linkages in peptide and protein in HTW.

本研究首次报道了高温水中酰胺的水解反应机理以及水在酰胺水解过程中所起的作用，为肽键以及蛋白质在高温水的水解研究提供了科学依据。

In this paper, the acid hydrolysis of the protein extracted from the stickwater under the microwave conditions was studied, and the optimal hydrolysis conditions were selected.

通过采用微波对废水鱼汁中蛋白质进行酸水解的研究，对废水鱼汁中蛋白质水解的工艺条件进行了优化。

The taste components comes from incomplete fermented residual sugar and dextrine, glycerin by fat splitting, amino acid by protein, acid and delicate flavour materials.

中添加的焦糖量、陈化时间不同而异及化学反应而呈色；②香来源于原料、麦曲、生产工艺及贮存发生的反应中；③味的形成及来源于未完全发酵的残糖和糊精、脂肪水解产生的甘油、蛋白质产生的氨基酸、酸和鲜味物质。

An alkaline protease Alcalase AF 2.4 L was used to hydrolyze corn gluten meal and study the effects of pH and enzyme concentration on protein conversion. The optimum technological conditions were obtained in this test as follow: hydrolyzing temperature 55℃,addition of 2 ml alkaline protease(90 947 U/ml),pH=9,concentration of substrate 0.1g/ml,hydrolysis time 1 h.

研究了碱性蛋白酶Alcalase AF 2.4L水解玉米蛋白时，pH值和酶浓度对蛋白质转化率的影响，得到了实验范围内的最佳工艺条件：水解温度55℃，碱性蛋白酶加入量2ml(90 947U/ml)，pH值9，底物浓度0.1g/ml，水解时间1h。

The ABA raised activity of protease, RNase, DRNase in detached leaf and speeded up hydrolysis of protein and nucleic acid, loss of enzyme protein of the RuBPcase. The Zeatin was in apposition and antagonised negative effects of the ABA.

ABA可刺激离体叶片中蛋白质水解酶RNase、DRNase活性的上升，加速蛋白质和核酸的水解，特别是RuBP羧化酶蛋白的丧失，Zeatin则相反并且抵消ABA的负作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。