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Proteinases and peptidases are involved in the hydrolysis of protein during digestion, and have...

蛋白质酶和肽酶在消化过程中参与蛋白质的水解,而且具有重要的生理学和病理学作用。

The results showed that there was 87.92% of protein in the hydrolyzate.

结果表明,酶水解物的产率为17.8%,酶水解物中蛋白质的含量为87.92%;10 g/L虾夷扇贝卵巢酶水解产物能消去DPPH自由基61.68%的活性。

Using papain to hydrolyze the legumin and vicilin respectively showed that it could obtain higher DH from legumin than from vicilin, and the emulsifying activity of legumin hydrolysates was also increased more than vicilin hydrolysates. It was easier to hydrolyze the β-sheet fractions than α-helical fractions of broad bean legumin by papain, but to vicilin the β-sheet fractions was difficult to be hydrolyzed.

发现Papain对11S的水解程度大于7S,11S水解物的乳化活性也高于7S水解物;对水解物的圆二色性分析表明:蚕豆11S球蛋白的β-折叠结构易于被木瓜蛋白酶作用,而7S球蛋白的α-螺旋结构易于被木瓜蛋白酶作用;蛋白质分子中的α-螺旋结构对乳状液的形成和稳定起着重要作用。

The optimum hydrolytic conditions of razor clam for neutral protease were : enzyme concentration 2.4%, hydrolysis temperature 50癈, substrate concentration 1:3, pH 7 and reaction time 6 hrs with the degree of hydrolysis and extraction rate of total nitrogen 42.46% and 83.04% respectively; and foracid protease they were: en/yme concentration 6.0%, hydrolysis temperature 50癈, substrate concentration 1:4, pH3.5 and reaction time 5 hrs with the degree of hydrolysis and extraction rate of total nitrogen 46.37% and 87.94% respectively, the hydrolysate being clear light yellow. For the combination of two kinds of proteases they were: first hydrolyzing with 2.6% f 1 avouryme for 3 hrs, then hydrolyzing with 2.4% neutral protease for 3 hrs with the degree of hydrolysis and extraction rtiie of total nitrogen 4-1.57% and 86.95% respectively.

最后综合考虑了水解率、总氮回收率和水解液的色泽、澄清度等指标,得出了较适宜的酶种为单酶以枯草杆菌中性蛋白酶和酸性蛋白酶效果较好,双酶以风味蛋白酶与枯草杆菌中性蛋白酶双酶组合水解效果较好,其适宜的水解工艺条件分别为:枯草杆菌中性蛋白酶加酶量为2.4%,水解温度50℃,料水比1:3,pH值中性条件下水解6h,绕蛙蛋白质的水解率和总氮回收率分别为42.46%和83.04%;酸性蛋白酶水解编蛙肉的适宜的工艺条件为加酶量6.0%、水解温度50℃,料水比l:4,pH值3.5条件下水解sh,水解率为46.37%,总氮回收率为87.94%,水解液呈较清澈的淡黄色;双酶采用先用2.6%风味酶水解3h后,再加入2.4%中性蛋白酶水解3h,水解率达44.57%,总氮回收率为86.95%。

However, the breadmaking experiment on wheat flour of 12 wheat varieties shows that the wheat protease has a remarkable effect on the breadmaking properties of wheat flour under the acid condition, which is in well connection with the quantity and quality of protein itself.

对小麦内源蛋白水解酶与小麦粉中蛋白质的水解作用和环境的pH值关系进行了研究,结果表明小麦内源蛋白水解酶在中性条件下,对蛋白质不产生强烈的水解作用;在酸性条件下,对蛋白质有显著的水解作用。

Proteins associated with the membrane and the cytosolic portions of the proteome under different copper ion concentrations were differentially labeled with an acid cleavable isotope-coded affinity tag reagent, and the labeled peptides were separated by two-dimensional chromatography and characterized by electrospray ionization-tandem mass spectrometry.

我们分别将在不同铜离子浓度下的细胞质以及细胞内膜中所有蛋白质标识上酸裂性同位素亲和性标识(acid cleavable isotope-coded affinity tag, cICAT)试剂,用二次元管柱层析将所有标识并酵素水解过的片段分离,并且用电洒式串连质谱(electrospray ionization-tandem mass spectrometry, ESI-MS/MS)以及基因体定序资料库决定蛋白质序列。

Response surface analysis(central composite design of uniform precision) of SAS 9.1.3 system was applied to optimize the prime four factors(the ratio of stuff and water, the quantity of enzyme ,the time of fermentation and the quantity of inoculation)for solid fermentation of soybean meal in order to researching the contributiveness of factors and their interactions to the degree of hydrolyzation of soybean protein.

采用SAS 9.1.3中的响应面分析法对影响豆粕固态发酵中蛋白质水解的四个主要因素(料水比,加酶量,发酵时间,接种量)进行了优化,考察了各因素及其交互作用对大豆蛋白水解度的影响。

The technological conditions of enzymic hydrolysis of potato residues by α-amylase and proteinase were investigated.

以干薯渣为原料,采用酶法水解淀粉、蛋白质的提取方法,探讨了薯渣中淀粉、蛋白质水解的工艺条件。

Some parameters that affect the properties and yield of hydrolysate, such as the amount of the prote...

研究结果表明:采用此方案 Cr2 O3在水解蛋白质中的含量仅为 10 -6 级;水解蛋白质的回收率为 4 5 %左右,蛋白质总回收率达到 85 %左右

Cells use molecular chaperones and proteases to implement the essential quality control mechanism of proteins.

细胞对蛋白质的质量控制是生命过程的关键机制之一,这是通过分子伴侣和蛋白质水解酶这两类蛋白质的功能而具体实现的。

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