水蛭

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

Any of various worms or wormlike animals of the phylum Annelida,characterized by an elongated,cylindrical,segmented body and including the earthworm and leech.

环节动物环节动物门的一种虫或似虫的动物，其特征为身体较长，呈圆柱形分节状，包括蚯蚓和水蛭

Methods: Radix Rheum, Leech, Fructus Arctium in Huangzhi Oral Liquids was identified by TLC.

采用薄层色谱法鉴别黄蛭口服液中的大黄、水蛭、牛蒡子。

Results: The obtained TLC spots of Radix Rheum, Leech, Fructus Arctium in Huangzhi Oral Liquids occurred at the corresponding positions compared to the controls. The studies showed that spot of naringoside on TLC.

结果：黄蛭口服液中的大黄、水蛭、牛蒡子和对照药材薄层色谱主斑点位置一致；且定性鉴别斑点圆整，分离度好，易于区别。

Bushen Zhuanggu granules were consisted of deerhorn glue, Chelonia Glue, Leech and Common Yan Rhizome; Xianling Gubao capsule was offered by Guizhou Tongjitang Pharmaceutical Co. Ltd.

补肾壮骨颗粒主要由鹿角胶、龟胶、山药、水蛭等药物组成；仙灵骨葆胶囊由贵州同济堂制药有限公司提供；维甲酸由武汉远城科技发展有限公司提供。

Bushen Zhuanggu granules were consisted of deerhorn glue, Chelonia Glue, Leech and Common Yan Rhizome; Xianling Gubao capsule was offered by Guizhou Tongjitang Pharmaceutical Co.

补肾壮骨颗粒主要由鹿角胶、龟胶、山药、水蛭等药物组成；仙灵骨葆胶囊由贵州同济堂制药有限公司提供；维甲酸由武汉远城科技发展有限公司提供。

Lannuo is the first to apply Natural Hirudin in the cosmetic, health foodstuffs, curatorial fields in the world.

lannuo是第一个报名的天然水蛭素在化妆品，保健食品，医药等领域，在世界上。

Abstract］ Objective To establish the extraction technology of hirudo.

目的 确立水蛭的提取工艺。

The technology is good for hirudo extraction.

该工艺适于水蛭的提取。

The technology is good for hirudo extraction.

该工艺适于水蛭的提龋

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。