水杨苷

The vitro methods include autoagglutination test,calcium dependency test,pyrazinamidase test,salicin fermentation,esculin hydrolysis,Congo red colony test and virulence plasmid.

体外法包括自凝试验、依钙试验、吡嗪酰胺酶试验、水杨苷发酵、七叶苷水解、刚果红菌落试验和毒力质粒。

These two isolates presented typical characteristics of Streptococcus iniae with gram-positive cocci in chains, catalase negative and beta-hemolytic, growth under 10℃ and non-growth up 45 ℃, hydrolyzation of esculin and arginine, Voges-Proskauer, urease, and hippurate tests negative, fermentation of glucose, salicin, sucrose and starch, non-fermentation of arabinose, inulin, lactose, melibiose, raffinose and sorbitol.

实验结果表明，两菌株具有典型的海豚链球菌特性：在显微镜下为球形细胞，呈长短不一的链状排列，革兰氏染色阳性：在血琼脂平板上显示β溶血；1O℃生长，45℃不生长；接触酶阴性；水解七叶苷、精氨酸，VP试验、脲酶和马脲酸试验阴性；发酵葡萄糖、水杨苷、蔗糖和淀粉，不发酵阿拉伯糖、菊糖、乳糖、蜜二糖、棉子糖和山梨醇等。

The tree contains a chemical called salicin. In the eighteen hundreds

这种树包含着一种名为水杨苷的化学物质。

The tree contains a chemical called salicin. In the eighteen

这种树包含着一种名为水杨苷的化学物质。

Making standard curve by salicin enzyme activity ,we can get the formula of relative and absolute enzyme activity ,then transform into absolute enzyme activity of glucose.

通过水杨苷法作葡萄糖标准曲线，能得到相对酶活与绝对酶活的换算公式，进而得到绝对酶活。

The tree contains a chemical called salicin.

这种树包含着一种名为水杨苷的化学物质。

A spectrophotometric method to measure the concentration of salicin in the water extract of willow bark was established based on the HCLO4 - Vanilin staining reaction with λmax of 520 nm.

以水为溶剂，50℃下超声提取柳树茎皮中的水杨苷，采用香草醛分光光度法，在吸收波长520nm下，建立水杨苷含量的测定方法。

The ancient Greeks, for example, treated pain with a tea made by boiling willow leaves and bark, a tea modern scientists now know contains salicin, a precursor of acetylsalicylic acid—aspirin.

例如，古希腊人用柳叶和柳树皮煮成的茶水来止痛。现代科学家已经了解到，这种茶里面含有水杨苷成分，就是乙酰水杨酸——阿司匹林的前身。

In this research, the activities of CWDE were measured by saccharifying according to the theory that red-brown amino compounds were produced when 3, 5-dinitro salicylic acid and aldehyde were heated together. And the testing conditions were made clear. The activity of Cx should be tested with optimal temperature of 50℃, optimal pH of 4.8, incubation time of 30 min, and optimal substrate of 0.6% CMC-Na. Due to the heat-libality, Cx should be sterilized more than 10 min. The testing conditions of the activity ofβ-G were almost the same with Cx except the substrate of 0.5% salicin and the sterilization time of more than 15 min.

主要取得了一下成果：(1)明确了条锈菌细胞壁降解酶活性测定的条件：羧甲基纤维素酶活性的测定条件为50℃，pH4.8，反应时间30min，底物浓度为0.6%羧甲基纤维素钠，以DNS法测定，且失活酶液的灭活时间应大于10min；β-葡萄糖苷酶活性的测定条件为：50℃，pH4.8，反应时间30min，底物浓度为0.5%水杨苷，以DNS法测定，且失活酶液的灭活时间应大于15min；果胶酶活性的测定条件为：50℃，pH4.8，反应时间1h，底物浓度为0.25%果胶，以DNS法测定，且失活酶液的灭活时间应大于10min。

The purified recombinant enzyme had optimal reaction conditions at pH 5.0 and 80 °C, was stable between pH 4.0 and pH 6.0. The enzyme had residual activity of 60% at 95 °C for 6 h at pH 5.0, thus has considerable potential in industrial applications. The specific activities of the enzyme to salicin, p-Nitrophenyl β-D-glucopyranoside, p-nitrophenyl β-D-xylopyranoside and p-nitrophenyl α-D-glucopyranoside were: 57500 U/mg, 1632 U/mg, 109 U/mg, and 39 U/mg, respectively, the specificity of salicin was the highest, p-nitrophenyl β-D-glucopyranoside followed, no activities to xylan and arabinofuranoside.

重组 Tm-SIGlA 的酶学性质研究表明：该酶最适作用温度和最适作用 pH 分别为80 ℃和 pH 5.0；在 pH 4.0-6.0 之间酶活性稳定，95 ℃保温 6 h 残留活性为 60%，具有非常高的热稳定性；在所测定的底物中，该酶对水杨苷、对硝基苯酚-β-D-葡萄糖苷(p-Nitrophenyl β-D-glucopyranoside)、对硝基苯酚-β-D-木糖苷(p-Nitrophenyl β-D-xylopyranoside)和对硝基苯酚-α-D-葡萄糖苷(p-Nitrophenyl α-D-glucopyranoside)的比酶活分别为：57500 U/mg、1632 U/mg、109 U/mg 和39 U/mg，作用水杨苷的专一性最强，其次为对硝基苯酚-β-D-葡萄糖苷，没有检测到对木聚糖和阿拉伯糖苷的活性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐