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After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对,结果显示,PPV-SC1 NS1与其他的PPV NS1的同源性较高,仅存在个别的差异,分别是第39位A→G,第153位T→C,第175位A→G,第1117位A→C,第1535位A→C;同源搜索比较表明,PPV-SC1与PPV NS1同源性可达98%、99%,与其他的细小病毒NS1基因也存在很大的保守性;密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性;PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段,用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区,返回的结果并没有得到有显著意义的跨膜区的存在;根据基于motif数据库的结构域预测,PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP/GTP结合位点,该蛋白还存在16个蛋白激酶C磷酸化位点,21个酪蛋白激酶2磷酸化位点,3个cAMP-/cGMP依赖蛋白激酶磷酸化位点,PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域,推测NS1可能与POX_D5有类似的功能。

High absorption and retentions clinoptilolite /Starch/ PAANa composite was synthesized by water-solution copolymerization with clinoptilolite and acrylic acid as principal raw materials,with N N-methylene-bis-acrylamideand as crosslinker, and with potassium persulphate as initiator .

以丙烯酸、斜发沸石为主要原料,以N,N'-亚甲基双丙烯酰胺为交联剂、过硫酸钾为引发剂,采用水溶液聚合法合成了斜发沸石/淀粉/聚丙烯酸高吸水保水性复合材料。

In south of the East China Sea, high abundance area of Ostracods was located in offshore in winter and spring but nearshore in summer and autumn.

东海浮游介形类基本上是一大类较为典型的暖水性且具有集群行为的海洋浮游动物。

PTFE is a typical low-surface-energy material: The CA for water on a smooth PTFE film is about 121.61.88, and for diesel oil it is 11.21.68, that is, PTFE is intrinsically hydrophobic and oleophilic.

聚四氟乙烯是一种典型的低表面能材料:核证机关水在光滑的PTFE膜约121.6?1.88,和柴油是11.2?1.68,也就是说,聚四氟乙烯在本质上是疏水性和亲油性。

Among the nektons, freshwater species (10 species) were the most abundant ecological groups, followed by estuarine (6 species), migratory (3 species), and coastal species (1 species).

生态类群主要以淡水性种类(10种)和河口性种类(6种)为主,洄游性种类(3种)和海洋性种类(1种)较少。

According to information available, the current water-based Wood consumption in China for less than 3%, distribution of the Wood Oil accounted for 97% of dealers, distributors Wood water-based scale is far worse than in the Wood Oil

但是从长远来看,由于水性木器漆已经切中了家装涂料市场未来发展的脉搏--健康环保、无刺激气味,以安全健康倍受青睐,随着消费者对环保的期望值越来越高,对人体无毒无害环保全新理念的灌输,作为涂料化工企业也不得不为迎合消费者的需求而销售。

Wood's painting to distinguish between oil and mixed oil, which is divided into Pu Tongqi edible vegetable oil and water-based paint, treated in different ways.

木工的刷漆分清油和混油,其中清油又分普通漆和水性漆,处理的方式各有不同。

Companies to introduce advanced high-temperature glaze raw materials, including glass, environmentally friendly water-based glass paint, glaze applied to glass bottles.

公司引进国内先进原材料包括玻璃高温釉,水性环保玻璃漆,应用于玻璃酒瓶上釉。

The soil moisture characteristics, dynamic variation and spatial variation in the fields for 4 soils in the loess area of Gansu province, maximum hygroscopicity, wilting point moisture and water holding capacity were studied. The dynamic variation of soil moisture shows "two peaks -two valleys" type.

对甘肃黄土地区4个土类的土壤水分特征、动态及空间变异规律,以及最大吸湿水、凋萎湿度和持水性能的研究表明,土壤水分动态变异规律呈&双峰-双谷&型,据此在时序上可划分为5个明显的时期:(1)春季增失墒交替期;(2)春夏严重失墒期;(3)夏秋墒情恢复期;(4)秋冬缓慢失墒期;(5)冬季墒情稳定期。

The film offsets the traditional polymer-cement composite material"s defects,such as lack of flexibility and the film"s re-solution,and enhances the tensile strength of the coating.

该涂膜弥补了水泥基材料柔性不足以及聚合物乳液涂膜再溶胀,防水性差的缺陷,提高了聚合物涂料的拉伸强度。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。