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To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体,本实验通过蛋白疏水性抗原性分析设计多肽抗原,用其免疫家兔获得抗血清, ELISA检测其效价为1:10 000; Western blotting法检测发现,通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性;用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示:磷酸化的Ser183在不同细胞中表达差异不显著,而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Also Cooperating with Germany TP Maschinentechnik to produce Katana and Exculibur Water Base Film Laminator in Taiwan to serve worldwide Market.

与德国TPM公司合作依原设计制造katana 及 Exculibur系列高速水性覆膜机,整合销售生产服务全球市场。

The particle size and flow behavior of the latexes,dynamic mechanical properties,tensile behavior,and water resistance of the copolymers,as well as the nucleation mechanism of latex particles were investigated.

对乳胶粒粒径大小、乳液流体力学行为、共聚物的动态力学性质、拉伸行为及耐水性进行了研究,并对乳胶粒的成核机理进行了探讨。

The blends include the combination of one latex and another latex, especially hard latex and soft latex, latexes and water-soluble polymers, polymers with different chemical composition, and so on.

水性涂料用树脂的混合主要包括乳液和乳液的混合,特别是硬乳液和软乳液的混合,乳液和水溶性树脂的混合,不同化学成分树脂的混合等。

The results show that the hydrophobicity of reaction medium has great impact on the activity and enantioselectivity of lipases, and isooctane was the best reaction medium.

实验结果显示疏水性溶剂对於酵素反应活性和镜像选择性有较大影响,而异辛烷为最好的反应介质。

The water throws over covers transfers the India technology uses one kind is easy to dissolve comes the load bearing chart article to the under-water river character thin film.

水披覆转印技术使用一种容易溶解于水中地水性薄膜来承载图文。

SEC_S 100 is a water-based system specially designed for concrete or mortar suraces to attain an aesthetic loor through applying the pigmented water-based epoxy resin.

SEC_S 100水性环氧涂装地坪是在混凝土或砂浆地面上把着色合成无溶剂树脂涂抹上去进而美装地面的工法。

A novel ultrastable particle-stabilized foam slurry was prepared by alumina particles modified by the short amphiphiles to make them partially lyophobic and to initiate direct foaming with vigorous stirring.

采用短链两亲分子戊酸修饰氧化铝颗粒,使其部分具有的疏水性,在机械搅拌的作用下,形成了粒子稳定型泡沫(particle-stabilized foam),制备了一种新型的超稳定陶瓷泡沫浆料。

Results showed that TECAM only extracted the freely dissolved HOCs and the presence of DOC in water reduced the availability of HOCs to both TECAM and Japanese medaka. The effect of DOC on the accumulation profiles of the three target compounds in TECAM and medaka was similar.

结果表明,TECAM只富集水中自由溶解形态的疏水性有机物;水中存在的溶解性有机碳会降低3种有机氯农药被TECAM和青鳉富集的可利用性;TECAM和青鳉的富集结果有很好的可比性。

Zuxing has agencies in more than 20 countries and districts. Zuxing has three state-of-the-art manufacturing plants with a total land area of 50,000 sq.m., of which 30,000 sq.m is for plant site and more than 400 dedicated employees. Zuxing has been awarded ISO9001:2000 and ISO14001 certifications. Zuxing is capable of new product research and development. The main products are non-leafing aluminium pigments, water-borne aluminium pigments, resin-coated aluminium pigments, aluminium pigment powder, vacuum metallised flake suspension, pelletised aluminium pigments for plastics and pelletised aluminium pigments for inks.

公司现有三个生产工厂,占地总计约50,000平方米,有30,000平方米的现代化专业厂房,职工400多人,已通过ISO9001:2000质量管理体系和ISO14001环境管理体系的认证,严格控制产品的稳定性,有很强的新产品开发能力,重视走精品路线,主要产品有:普通非浮型铝银浆、水性铝银浆、树脂包覆型铝银浆、粉末状铝颜料、电镀级铝颜料、塑胶专用条状铝颜料、油墨专用条状铝颜料等。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。