水平状态

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

By analyzing the loading status of three-point drop test platform, the force model of which has been built, and determined the factors that affect the measurement precision of horizontal load of wheel, derived the computational formula of horizontal load of wheel, so, the original measurement method of horizontal load of wheel has been improved.

通过分析三点支撑落震平台的受力状态，建立了三点支撑落震平台的力学模型，确定了影响机轮水平载荷测量精度的因素，推导出了机轮水平载荷的计算公式，从而改进和完善了现有的机轮水平载荷测量方法，使机轮水平载荷的测量更精确、更合理。

Methods Forty women and their babies (40) were enrolled in this study. Placental tissues were assayed for leptin mRNA by reverse transcription/polymerase chain reaction, and assayed for the obese gene protein leptin by Western-blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio-immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery.

方法在40例产妇分娩时采集胎盘与脐血，采用逆转录聚合酶链反应检测胎盘肥胖基因mRNA相对表达水平，采用Western-Blot检测胎盘肥胖基因蛋白表达水平，采用免疫组化观察胎盘肥胖基因蛋白表达位点，采用放射免疫法检测脐血瘦素水平，采用Ponderal指数［PI=100×体重/身长3］估测新生儿营养状态。

Firstly, we collected and sorted out the GPS data, established the uniform velocity field for the Chinese mainland and the surrounding regions, and the horizontal deformation field relative to the Eurasia plate; Secondly, we established a motion model for the entire rotation and homogeneous strain of the first and second level blocks in Chinese mainland, analyzed the motion pattern and strain behavior of active blocks in Chinese mainland, as well as the motion pattern of principal fault zones in Chinese mainland; Thirdly, considering the heterogeneity of strain distribution inside a block and supposing that the strain tensor inside the block is the linear function of location, we derived and established an equation for the entire rotation and linear strain of block and a motion model for the entire rotation and linear strain of principal blocks in Chinese mainland; We also calculated the principal strain field and the maximum shear strain field in Chinese mainland and the surrounding regions, analyzed the basic characteristics of strain field in Chinese mainland, as well as the relationship between the spatial distribution of strain field and large tectonic zones; Finally, we established the 2-D geotectonic model and digital model for Chinese mainland and the surrounding regions, calculated the stress field in Chinese mainland and the surrounding regions, and analyzed the basic features of stress field in Chinese mainland and the principal driving force for the motion and deformation in Chinese mainland.

收集整理了已有GPS资料，建立了中国大陆及周边地区统一的速度场以及相对欧亚板块的水平形变场；建立了中国大陆一、二级地块整体旋转与均匀应变的运动模型；分析了中国大陆活动地块的运动状态，应变状态，以及中国大陆主要断裂带的运动状态；考虑到一个块体内部应变张量空间分布的非均匀性，假设块体内部的应变张量是位置的线性函数，推导建立了块体整体旋转与线性应变方程，建立了中国大陆主要地块整体旋转与线性应变的运动模型；计算了中国大陆及周边地区的主应变场与最大剪应变场，分析了中国大陆应变场的基本特征，以及应变场的空间分布与大型构造带的关系；建立了中国大陆及周边地区二维的地质构造模型和数值模型，计算了中国大陆及周边地区的应力场，分析了中国大陆应力场的基本特征以及中国大陆运动、形变的主要驱动力。

The sub-health status is one state between health and illness for body and mind.The body doesn\'t give an obvious disease, but the person feels uncomfortable in body,mentality and human communication, displaying one kind of physiology state that thevigor, reaction ability and acclimatization ability are weakening. This state is mainlycaused by the hypofunction on physiology and metaboly. It is not yet a disease, butalready seriously affect people\'s working efficiency and living standard.

亚健康状态是指人的身心处于疾病与健康之间的状态，是机体虽无明确的疾病，但在躯体上、心理上和人际交往上出现种种不适应的感觉和症状，从而呈现出活力、反应能力和对外界适应能力降低的一种状态，这种状态多由人体生理机能或代谢机能低下所致，虽然尚未达到疾病水平，但已严重影响人的工作效率和生活质量。

Want to describe a kind of appearance, a kind of appearance of average level, this kind of appearance comes to a decision true real strenght, if 12 emit point, perhaps 12 breakages of, can't resolve a problem.

想描述一种状态，一种平均水平的状态，这种状态决定了真实的实力，如果一两个冒尖，或者一两个破坏的，解决不了问题。

In experiment 2 we test the influence of grapheme and meaning process levels in the external source monitoring, and the result is that the error rates of the external source monitoring with meaning process level is worse than with grapheme but the difference just is margin marked

实验2，检验字形和语义加工水平对外部来源检测的影响，结果表明，语义水平加工状态下的外部来源检测错误率比字形加工状态下的差一些，但差异仅仅是边缘显著

Applied with test of potted plant and measurement absorbency for study physiological response of activity of cell defense enzymes and lipid peroxidation of slash pine's half-sib under water stress. There are 7 families comparison with general slash pine. The contents of superoxide dismutase, catalase, antioxidant, malondialdehyde and the rate of superoxide anion(-O2) generating are mensurated under normal water condition while soil water content is 70% and light water stress while soil water content is 55% to 60% and medium water stress while soil water content is 35% to 40% and heavy water stress while soil water content is 20% to 25%. The test result indicate that either water grads or families of contents of SOD,CAT,AsA,MDA are mighty notable difference. The rate of -O2 generating is notable difference within families. The rate of -O2 generating is mighty notable difference within water grades.

为研究湿地松优良半同胞家系保护酶及脂质过氧化作用对水分逆境的生理响应，采用盆栽试验和吸光度测定结合的方法，以普通湿地松种子为对照，测定了七个湿地松半同胞家系在正常水分状态（土壤相对含水量为70%）、弱度水分胁迫（土壤相对含水量为55%~60%）、中度水分胁迫（土壤相对含水量为35%~40%）和强度水分胁迫（土壤相对含水量为20%~25%）条件下的SOD、CAT、AsA、MDA的含量和-O2产生速率，研究结果表明：无论是不同水分梯度还是不同家系水平，SOD、CAT、AsA活性和MDA含量的差异均达极显著水平，不同家系水平间-O2产生速率有显著差异，不同水分梯度间-O2产生速率差异达极显著水平。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。