氰胍

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以发根农杆菌A4菌株诱导的人参发根为材料，用改良的异硫氰酸胍法提取总RNA，得到了纯度好的完整的总RNA。

Total RNA is isolated from rice caryopses displaying different development status by using the methods of TRIZOL Regent, Guanidine Thiocyanate and improved SDS.

利用TRIZOL试剂快速提取法、异硫氰酸胍法和改良的SDS法提取不同发育时期水稻颖果的总RNA，并通过凝胶电泳、紫外分光光度法检测提取获得的RNA样品的品质。

Extraction of high-quality RNA from Panax ginseng tissues is particularly difficult due to high levels of polyphenolics and polysaccharides, or other compounds that bind and/or coprecipitate with RNA. The methods such as guanidinum thiocyanate, CTAB and modified Trizol were applied to isolate high purity and integrity RNA from Panax ginseng.

针对人参组织多酚、多糖类物质含量较高的特点，比较了改良的异硫氰酸胍法、改良的CTAB法和改良的Trizol法等3种不同的RNA提取方法。3种改良的方法均能从人参组织中提取到总RNA。

Using large sample DNA of capacity technology, the maize high lysine content of SSR mark analyse technological platform has been established in this paper. The main results are as follows: the five comparing research methods of drawing maize DNA mark have shown that the method of iso- sulphur cyanic acid guanidine drawing maize genome DNA has the superiority of using less medicine, operating sequence more simple , faster, and costs lower , steady and reliable quality of DNA, etc..Utilizing this method , one person can draw 300-400 DNA samples each workday.lt indicates that the method can be used in SSR marker of maize,.

本文从大样本容量的DNA快速提取技术入手，初步建立了玉米高赖氨酸含量的SSR分子标记分析技术平台，获得的主要结果如下：对五种玉米DNA提取方法的比较研究认为，异硫氰酸胍法提取玉米基因组DNA，具有使用药品少、操作步骤简单、快速、成本低、DNA质量稳定可靠等优点，利用该方法，每人每个工作日可以提取300～400个DNA样品，可以在玉米SSR标记分析中应用。

RESULTS Duodenal ulcer was the first cause of upper gastrointestinal hemorrhage. Gastric ulcer, cirrhosis, Mallory-Weiss syndrome or acute gastric mucous lesion was the second cause of the illness alternatively. The rate of endoscopy and emergent endoscopy has been going up year after year and got up to 80% in 1998. Bleeding and active hemorrhage, respectively were found in 24.3% and 14.9% of the patients underwent endoscopy before medication. Endoscopic hemostasis was performed in 108 patients by spraying hemostatics and a good response was achieved subsequently. Hemostatics were mainly cimetidine and yunnan baiyao before 1994. Reptilase, pPI, famotidine and ranitidine were used increasingly while cimetidine and yunnan baiyao were used decreasingly. Octreotide was used in patients with cirrhosis commonly. The length of hospital stay and the period from admission to bleeding cease tended to be shorter year by year, but the reduction rate was small. The uses of ranitidine, famotidine, pPI, reptilase, octreotide, early endoscopy and endoscopic therapy may play a role in improving the therapeutic effect of hemostasis, reducing the hospitalization and the mortality.

结果 十二指肠球部溃疡是历年上消化道出血的首要原因，胃溃疡、肝硬变、Mallory -Weiss综合征、急性胃粘膜病变则交替成为第2位出血原因；内镜诊断率和急诊内镜率逐年提高，1998年达到80%，内镜下见出血者占内镜检查的24.3%，而见到活动性出血者仅占内镜检查者的14.9%；内镜下止血108例次，成功率100%；1994年前治疗药物主要为云南白药和甲氰咪胍，之后，随着立止血、质子泵抑制剂、法莫替丁、雷尼替丁等药物使用的逐渐增多，前二者的使用逐渐减少，善得定多用于肝硬变出血；住院天数和止血天数呈逐年下降的趋势，但幅度微小；雷尼替丁、法莫替丁、PPI、立止血、善得定的使用以及及时的内镜检查和内镜下治疗在提高止血效果、缩短住院天数、降低死亡率方面可能起到了一定作用。

ABSTRACT : Objective To establish a HPLC method for determination of impurity dicyanodiamide and impurity(1) and impurity(2) in Metformin hydrochloride and Glibenclamide Tablets.

目的建立 HPLC 方法测定复方盐酸二甲双胍格列苯脲片中已知有关物质双氰胺杂质(1)和杂质(2)的含量。

In this paper, the structures and the synthesis methods of glycosyl isothiocyanates are summarized, including the application as intermediates in the preparation of glycosylthioureas, glycosylheterocycles, glycosylguanidins and glycosylconjugates and so on.

本文对糖基异硫氰酸酯的结构、制备方法，以及作为中间体在糖基硫脲、糖基杂环、胍基糖苷、糖基缀合物等重要化合物的合成中的应用作了较详尽阐述。

A modified guanidinium isothiocyanate method was used to extract total RNA from two forest insect species Clostera anastomosis and Saperda populnea.

用修改了的异硫氰酸胍法从两种森林昆虫Clostera anastomosis和Saperda populnea的不同组织中快速提取总RNA。

To prevent relapse cimetidine shoud not be ceased early.

在消化性溃疡引起的出血病人中，甲氰咪胍治疗是有效的，但为了防止复发，不宜过早停药。

The Promega commercial DNA extraction kit was used as control. Results The Promega kit resulted in a higher copy number of COWP gene[（6.45-9.86）×106]than that of Generay commercial DNA kit [（2.38-3.69）×106 ], 5% guanidine thiocyanate[（1.27-21.29）×105] or 2% Triton X-100 [（2.06-866.70）×103], respectively.

结果 Promega试剂盒的裂解液裂解隐孢子虫卵囊的效果最好，纯化所得卵囊COWP基因拷贝数可达（6.45～9.86）×106；其他依次为捷瑞试剂盒的裂解液[（2.38～3.69）×106]、5%异硫氰酸胍[（1.27～21.29）×105]和2% Triton X-100[（2.06～866.70）×103]。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。