氰胍

An abnormal condition caused by excessive intake of fluorine ,as from fluoridated drinking water,characterized chiefly by mottling of the teeth.

氟中毒由于过量摄入氟而导致牙齿产生色斑的不正常状况，如饮用氟化的水全氟胍可以由于的三聚氰酰胺，用5%的氟在约－16℃直接进行氟化制得。

Lysis solutions from imported and domestic genomic DNA purification kits,and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts,followed by different combining forms of freeze-thawing,proteinase K and sonication.Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene.HCT-8 cells were infected by C.andersoni oocysts.Real-time PCR was used to determine the enhancement effect of fetal calf serum,glucose,ascorbic acid,folic acid,calcium pantothenate and insulin while added into RPMI-1640 medium.

在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍，辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊，通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein，COWP)基因拷贝数。

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

According to the prior research , a highly efficient ext raction method was modified1 High2concent ration and DNA 2f ree RNA could be attained by using CTAB/ Guanidine Thiocyanate ext racion buffer1The total RNA can reach 1.7～2.0 of the OD260/ OD280 ratio with the yield of more than 015mg per gram of f resh tissues1 The method is suitable for high2quailty total RNA ext raction in leaves , root s , corollas , seeds and developing fibers.

本研究在前人方法的基础上对快速提取棉花组织总RNA 的方法进行了探讨。利用改进的CTAB 异硫氰酸胍裂解缓冲液，获得的RNA完整性好，无 DNA 污染，含量高，每克鲜组织能提取015mg 总RNA ， OD260/ OD280比值均在117～210之间。

All single step method and CTAB method did not work well when banana total RNA was isolated, unless modified single step method was used, in which, polysaccharide was removed by aether extraction prior to RNA precipitation.

对于富含多糖的香蕉果肉，一步法和CTAB异硫氰酸胍法的效果均不理想；改良后的一步法中增加了乙醚去除多糖并获得了较好的效果。

The total RNA were extracted from CDV infected DK cells or the tissues of infected animals by single step method of acid guanidinium thiocyanate-phenol-chloroform extraction and was used for reverse transcription.

用异硫氰酸胍-酚-仿一步抽提法提取细胞总RNA进行反转录，再以此产物为模板进行PCR扩增，筛选出最佳扩增条件。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

Methods The template DNA was extract from the human osteosarcoma cells line U－2OS by the single- step isolation method with isothiocyanic acid guanidine, the cDNA coding for the mature fragment of BMP－4 was amplified by the reverse transcription- polymerase chain reaction.

方法采用异硫氰酸胍一步法从人骨肉瘤细胞株U-2OS中提取总RNA，通过逆转录-聚合酶链反应扩增为BMP-4成熟区cDNA基因片段。

An improved guanidine thiocyanate method has been developed for total RNA extraction from the leaves and roots of the succulent xerophyte Zygophyllum xanthoxylum which contains high levels of polyphenolics and polysaccharides. In order to remove polyphenolics interference, 4%β-mercaptoethanol was added in the extraction buffer.

针对多浆旱生植物霸王富含多酚和多糖类物质的特点，在常规异硫氰酸胍方法的基础上进行改进，摸索出一种霸王叶和根总RNA提取的方法。

The purity and yid of RNA extracted by Trizol was better than guanidium thiocyanate.

Trizol 提取的大鼠肝RNA纯度、收率都优于硫氰酸胍方法提取的RNA。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。