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氯醛麻醉

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And the brains were got, post fixed, dehydrated gradiently in the alcohol, then impregnated andembedded in the celloidin, sliced into 90μm thick. The slices with the biggest dorsal hippocampus were stained by the alkali phosphatase method, then routine mounting.

对照组和实验组动物分别在给予生理盐水或KA7d后,水合氯醛麻醉,心脏灌流固定,取脑,后固定,酒精梯度脱水,火棉胶包埋,切片(厚90μm),碱性磷酸酶染色,常规封片。

Due to the unpredictability and safety concerns with chloral hydrate, we offered mask anesthesia as an alternate strategy.

考虑到水合氯醛的不可预测性和安全性,我们尝试面罩麻醉。

After 2 weeks, the rats were anesthetized with chloral hydrate and their blood pressures were measured.

喂养2 w后,水合氯醛麻醉,测量动脉血压。

Under suitable infusion rate (30 mg/kg/hr), the physiological conditions remained stable for 5 hr, while the results suggested that the high dose and vehicle were not appropriate dosages for a stable anesthesia. Also, fMRI activations of the contralateral primary somatosensory cortex were readily observed under low dosage of α-chloralose infusion.

在低剂量的α-氯醛醣麻醉下,动物的生理状态可以维持稳定达5个小时,并且在fMRI的实验中得到清楚的BOLD反应,但是对照组以及高剂量的老鼠,在这样长时间的麻醉中,生理及麻醉状况无法维持稳定。

10% chloral hydrate anesthesia and glass cannula enswathement are availability for mouse tail skin transplatation. The failure rate of this method is low. It is suitable for students experiment.

10%水合氯醛麻醉结合玻璃套管法包扎进行小鼠尾部皮肤移植是一种有效、简便、经济的小鼠尾部皮肤移植手术实验方法;此方法技术失败率低,适合学生实验课采用。

The recipients were divided into four groups which received 0.7%, 1%, 1.5% pentobarbital sodium and 10% chloral hydrate intraperitoneum injection respectively And the other recipients were divided into two groups which received glass cannula and adhesive bandages enswathement method, respectively.

分别用0.7%、1%、1.5%戊巴比妥钠和10%水合氯醛对小鼠进行麻醉试验,选出合适的麻醉方法;再进行小鼠尾部皮肤移植,对比玻璃套管法和创可贴法2种包扎方法的效果,以选出合适的手术方法。

Results The result showed that 0.7% and 1% sodium pentobarbital anesthesia duration was too short, 1.5% pentobarbital sodium easily made animal deaths, and 10% chloral hydrate used for anesthesia in mice was appropriate. And the glass cannula was more suitable than adhesive bandages for enswathement.

结果 0.7%和1%戊巴比妥钠麻醉持续时间过短,1.5%戊巴比妥钠和10%水合氯醛能维持足够手术操作所需的时间,但1.5%戊巴比妥钠易导致动物死亡,故采用10%水合氯醛麻醉小鼠较合适;玻璃套管法包扎的效果较创可贴法为好且更简便。

Changes of the blood gases and acid-base status of eleven healthy adult horses be-fore and after anesthetization kith Jing-Song-Ling,Chloral Hydrate and Laser were monitored with model DH-100G blood gas and acid-base analyzer.

以动脉血气分析做为手段,对静松灵、水合氯醛、激光三种麻醉方法进行评价,激光麻醉对实验马血气及酸碱值没有显著影响;静松灵(1mg/kg体重,im)和水合氯醛(128/100kg体重,iv)都能使PO_2、Sat·O_2、C-O_2等显著下降,但水合氯醛出现较早,在注射后30至60min时最低;而静松灵出现较晚,以注射后60~90min显著,但都未构成低氧血症,更未达呼吸衰竭。

In the chloralose-urethanized or curarized rabbits,infusion of saline or blood into the right auricle in amount of 8—10 ml/kg at the rate of 0.5—1.0 ml/sec induced bradycardia and hypotension.

一在氯醛糖和氨基甲酸乙酯麻醉的兔或未麻醉的箭毒化兔,右心房内输液(输入量为8—10毫升/公斤,输入速度为0.5—1.0毫升/秒),可规律地引起心率减慢和动脉血压下降。

The 4th left rib was cut and a model of left coronary artery occlusion/release was carried outo Rats were divided randomly into 4 groups: control group without LCA occlusion (group A, n=6);I/R group(group B, n=6); ketamine(5mg.kg-1)+I/R group (group C, n=6); ketamine(10mg.kg-1)+I/R group(group D,n=6)o All rats in groups B, C , D were subjected to 30 minutes of LCA occlusion followed by 120minutes of reperfusion. Rats in group C and D were injected with 5mg.kg-1 and 10mg.kg-1 of ketamine before reperfusion, respectively. Significant electrocardiogram and color changes at the area at risk were considered indicative of successful coronary occlusion and reperfusionc Serum was exampled from left jugular vein at 30 minutes and 120 minutes during reperfusion to measure IL-6 and TNF- level by ELISA measurements. After reperfusion of 120 minutes, the heart was removed and the cardiac apex was exampled with snap-frozen in liquid nitrogen and stored at -70 C .

采用左冠状动脉前降支结扎开放建立心肌缺血/再灌注模型,健康SD大鼠24只,随机分为心包打开假手术组(A组,n=6),缺血/再灌注对照组(B组,n=6),5 mg·kg~(-1)氯胺酮+缺血/再灌注组C组,n=6,缺血30min后右腹股静脉注入5 mg·kg~(-1氯胺酮,10mg·kg~(-1)KTM+I/R组D组,n=6,缺血30min后右腹股静脉注入10 mg·kg~(-1氯胺酮。10%水合氯醛40 mg·kg~(-1)腹腔注射麻醉,气管切开,连接多功能监护仪记录心电图;小动物呼吸机人工呼吸,呼吸频率60次/分,潮气量2ml/100g,于左胸第四肋间打开胸腔暴露心脏,在左心耳下1mm左冠状动脉处,用丝线,眼科外用不锈钢小圆针穿过心肌浅层,稳定10min后将U型含有铜丝的胶管置于冠状动脉表面一起结扎(A组不结扎,B、C、D组结扎);结扎开始左心室心尖部即由红色变暗,30 min后呈暗红色,心电图中出现S-T段抬高,说明缺血形成。

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