氯酸酯

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

One-pot reactions of 4 with isocyanates, triphenyl phosphine, hexachloroethane and triethylamine produced 2-anilino-5-arylidene-1-aryl-4,5-dihydroimidazo[1,2-b]-1',2',4'-triazol-4-ones 6 (or 7) in good yields.

而后用4与芳基异氰酸酯、三苯基膦、六氯乙烷和三乙胺"一锅"反应，得到4，5-二氢咪唑并[1，2-b]-1'，2'，4'-三唑-4-酮衍生物6或7。

Ciclesonide and beclomethasone dipropionate are administered as prodrugs activated by enzymes present in the lungs but not the oropharynx.

环索奈德和倍氯美松双丙酸酯作为肺内而不是口咽部的酶类激活的前药给药。

The working electrode was prepared by way of depositing TiO2 thin film on conductive ITO glass substrate via sol-gel method. As 3-electrode testing system was then formed combining it with SCE as reference one, black platinum electrode as auxiliary one and PC+LiClO4 solution as electrolyte.

采用溶胶－凝胶法在ITO导电玻璃基片表面制备一层TiO2薄膜，将其作为工作电极，与作为参比电极的饱和甘汞电极、作为辅助电极的铂黑电极、作为电解质溶液的碳酸丙烯酯＋高氯酸锂一起构成三电极测试体系。

In addition, it also produces reagents such as potassium bromide, Ludox and sodium metasilicate etc.

另有试剂溴化钾、章洗氯、钢锭模底板涂料、硅溶胶、偏硅酸钠及酒用香料酸酯类产品20余种。

In this thesis, monomer with unsaturated double bond was firstly synthesized byreacting acryloyl chloride with bifunctional monomer. Such synthesized monomer ormonomer with initial unsaturated double bond was used to prepare linear polymericmaterials by free radical polymerization. A series of new degradable space polymerswith acylamino bond were subsequently synthesized using diisocyanate as thecross-linker.

本文首先通过丙烯酰氯和双官能团单体反应引入不饱和双键，或者直接使用具有不饱和双键的单体，通过自由基聚合得到含有活泼羟基或氨基的线型高分子聚合物，再用二异氰酸酯作为交联剂交联，从而合成了一系列基于酰胺键的新型可降解高分子体型聚合物。

After acid hydrolysis of urine, primary amino acids were derivatized with ortho-phthaldialdehyde and removed from the sample by use of silica gel, followed by devivatized of hydroxyproline with 9-fluorenylmethyl chloroformate, and then injected into a C8 reversed-phase column.

采用邻苯二甲醛和芴甲基氯甲酸酯两个衍生剂，先后处理尿标本，以国产C8柱为固定相，在醋酸钠缓冲液中加入23%的乙腈作为洗脱液，3，4-脱氢脯氨酸作为内标。

This paperindicated that it was good for condensation reaction when benzene-ring bearingelectron-attaching groups. It also showed that and the 6-hydroxyl group could bereacted with acyl halide, haloid ether and halo alkene, but not with halo alkane. It wasnot only the first time to find that the reaction product of D-mannitol and aromaticaldehyde is tri-condensation derivatives, but also the first time to obtain thep-phthaldehyde-sorbitol derivatives containing eight hydroxyl groups.

研究发现：苯环上有吸电子基团时对缩合反应有利；多元醇缩醛衍生物6-羟基除与酰氯反应外，也能和卤代酸酯、溴代烯反应，但不与卤代烷反应；首次发现D-甘露醇与醛反应主要得到三缩合物；首次合成了含有8个羟基的对苯二甲醛和山梨醇的缩合物。

Flame— retardant resin was prepared by the condensation and polymerization of phosphoric acid, trihydroxy methyl propane and epoxy chloropropane,the resin was then reacted with hexane diacid and diglycol under certain condition ,resulting in polyol containing 1.5% P and 17— 18% polyester polyol, which was foamed with polyisocyanate, water and catalyst by one— step method, producing fire—resistant PU soft foam.

通过磷酸、三羟甲基丙烷和环氧氯丙烷的缩合和聚合反应制得阻燃树脂，然后将它与己二酸、一缩二乙二醇在一定条件下反应制得含P1.5%和含Cl17～18%的聚酯多元醇，再将它与多异氰酸酯、水、催化剂等组分采用一步法发泡工艺制得阻燃聚氨酯软泡塑料。

Benzoyl chloride was treated with potassium selenocyanate in dichioromethane by using polyethylene glycal-400 as the solid-liquid phase transfer catalyst to yield the corresponding benzoyl isoselenocyanate. This intermediate did not need to be isolated and directly reacted with arylamines, affording the N-benzoyl-N'-arylselenoureas 2a~2p. These compounds were characterized by IR, 1H NMR spectra and elemental analysis and the single crystal structure of 2k has been confirmed by X-ray crystallographic analysis.

以PEG-400为固液相转移催化剂，通过苯甲酰氯与硒氰酸钾反应合成了中间体苯甲酰基异硒氰酸酯，该中间体不需分离，直接与芳胺反应，得到N-苯甲酰基－N'-芳基硒脲(2a~2p)，这些化合物用1H NMR，IR及元素分析进行了表徵，并用X射线单晶衍射确定了化合物2k的单晶结构。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。