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An improved method that facilitates the extraction of PCR-compatible faecal DNA from giant panda\'s faeces is described. The method involves a novel preprocessing step in DNA extraction. The faeces is washed two or three times with precooled acetone, which removes numerous potential PCR inhibitors, and then digested with proteinase K. The DNA is purified with phenol/chloroform.

在粪便DNA的提取过程中采用一个新的预处理方法,将粪便用预冷的丙酮洗2~3次,除去粪便中含有的大量PCR抑制物,然后蛋白酶K裂解、酚-氯仿抽提,能提取到纯度很高的DNA供PCR扩增。

Peroxidases and phenol oxidases are the key enzymes in this process.

参与脱氯的关键酶系主要包括过氧化物酶和酚氧化酶。

Locks SSGP 850 is a fully synthetic soluble grinding fluid, which does not contain mineral oil, sulphur, chlorine, phosphate or phenol. Locks SSGP 850 is highly recommended in the precision grinding operation of cast iron, carbon steel, steel alloy, stainless steel, aluminum and its alloys, copper and its alloys.

洛斯 SSGP 850是全合成型水基磨削液,不含礦物油和硫、氯、磷、酚等物質,特別推薦應用於鑄鐵、碳鋼、合金鋼、不銹鋼、鋁和鋁合金,鋼和銅合金及非金屬材料的精密磨削加工。

Chen (1995) Differential effects of chloral hydrate and pentobarbital sodium on a cocaine level and its catecholamine response in the medial prefrontal cortex: a comparison with conscious rats, J.

标题中译:以三氯乙醛及巴比妥钠盐麻醉下之大白鼠其内前额皮质区之古柯碱浓度及其引起儿茶酚胺浓度上升之关系:和清醒大白鼠相比较。

Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠,血清进行肝功能检测;苏木素-伊红染色部分肝组织;Trizol-酚-氯仿一步法提取小鼠肝组织总RNA,紫外分光光度法测定总RNA浓度及纯度,实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达,以18S基因作为质控。

Objective To study the effect of anesthesia and affection on vital sign of propofol and ketamin.

目的 探讨异丙酚、氯胺酮在会阴部手术中麻醉的效果及对生命体征的影响。

The results indicated that phenols were the pollutants with high toxicity, in which pentachlorophenol had the highest toxicity in all these tested chemicals.

研究结果表明,在3类12种有机污染物中,卤代酚类物质对水生生物的毒性最强,其中五氯苯酚对水生生物具有极高毒性。

RNA was extracted with acid phenol-chloroform and precipitated with isopropanol.

RNA中酸性酚-氯仿和沉淀与异丙醇。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。