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DETERMINATION OF VINYL IN VINYL CHLORIDE ACETATE COPOLYMERS.

氯乙烯一乙酸乙烯共聚物中醋酸乙烯的测定

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

The results indicated that the highest yield of BOC-indigo was 97% in CH2Cl2, and the lowest one was in PGMEA. It showed that the yield of BOC-indigo increased with high polarity and low molecular volume of solvent.

从实验结果得知,以极性较强且分子体积较小的二氯甲烷作为反应溶剂,其合成隐性颜料产率可达到97.2%,较四氢呋喃产率多4.4%,且较丙二醇甲醚乙酸酯产率多7.4%。

Feed tableware disinfection, but food of use consecutively the rest 10-20 of a boil minute may use 500mg ∕ L effective chloric, or immerse with acid of 0.5% second crossing oxygen disinfection 0.5 - 1 hour; In all water intrusion wanting when tableware is disinfected, alexipharmic time calculates from boil case.

食餐具消毒,可连用剩余食物一起煮沸10-20分钟或可用500mg∕l有效氯,或用0.5%过氧乙酸浸泡消毒0.5-1小时;餐具消毒时要全部浸入水中,消毒时间从煮沸时算起。

The compositions of ethyl acetate fraction, normal butyl alcohol fraction, and water fraction differed significantly.

通过GC-MS和HPLC分析,黑米抗氧化提取物的石油醚部和氯仿部成分以脂肪酸为主,乙酸乙酯部、正丁醇部和水部之间成分构成差异较大。

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