氮尿

Objective To establish an RP-HPLC method for the simultaneous determination of 5 kinds of nonprotein-nitrogen metabolites in urine(urea, creatine, creatinine, uric acid and hippuric acid).

目的 建立RP-HPLC法同时测定尿液中尿素、肌酸、肌酐、尿酸和马尿酸5种非蛋白氮代谢产物。

By using electrophysiological technique, the effects of nitric oxide and atrial natriuretic peptide on renal afferent nerve activity induced by intrarenal arterial injection of endothelin-1(ET-1) were examined in anesthetized rat.

目的和方法：采用电生理学技术观察一氧化氮和心房钠尿肽对肾动脉内注射内皮索所致麻醉大鼠肾神经传入放电的影响。

TCM group were intragastric administrated with Shenluotong 25 g/kg.d, Control group and the model group were intragastric administrated with benazepril 10 mg/kg.d, Normal group and model group were given equivalent physiological saline.24 hour urinary protein,Scr and BUN of different groups, and the expression of CTGF mRNA in nephridial tissue were detected.

检测各组大鼠24 h尿蛋白、血清尿素氮、肌酐和肾组织CTGF mRNA的表达。

Results Compared with CRF group, nephrogenic hypertension of hemin group was reduced significantly at the 6th, 8th, 10th week after operation, and followed with the decrease of serum creatinine, BUN,urinary protein and the improvement of nephrogenic anemia at the 10th week after operation. Hemin not only increased the expression of HO-1, reduced the expression of ET-1 in the kidney, but also increased the concentration of carbon monoxide, reduced the level of ET-1 in the plasm.

同时，在术后第10周hemin组比CRF组血肌酐、尿素氮、尿蛋白显著降低(P＜0.05)，贫血明显改善(P＜0.05)；肾组织中HO-1表达明显增加，血浆中内源性CO水平明显升高，肾组织和血浆中ET-1水平明显降低(P＜0.05)。

aim: to establish a new reversed phase high performance liquid chromatography method for assay 4 kinds of non protein nitrogen markers of human diabetic nephro pathy, creatine, creatinine, urea and uric acid in dn patients urines.

目的：建立糖尿病患者尿液中与肾病相关的非蛋白氮代谢产物(肌酸、肌酐、尿素和尿酸)的反相高效液相色谱分离分析方法。

ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

选择健康杂种犬36只，无菌抽取犬骨髓液10 mL，经Percoll液密度梯度离心后贴壁法体外培养骨髓间充质干细胞，传2代后行5-氮胞苷诱导，5-溴脱氧尿苷标记备用。

FENa is typically less than 1%. However, in a patient taking diuretics such as furosemide, FENa may be greater than 1% even though the patient has prerenal azotemia.

FENa一般小于1%。但是，当肾前性氮质血症患者使用了利尿剂如呋塞米，FENa可能大于1%。

Methods:276 patients of psychogeny associated with brain disease were treated with olanzapine for 12 weeks.

对276例脑器质性精神障碍患者进行12周的奥氮平治疗，使用bprs、tess评定治疗效果，治疗前后查血常规、尿常规、肝功能、肾功能、血糖、血脂、心肌酶、心电图、脑电图、胸透检查。

Methods:276 patients of psychogeny associated with brain disease were treated with Olanzapine for 12 weeks. All subjects were evaluated with BPRS and TESS. And patients were checked blood and urine routine, liver and renal function, blood sugar, blood fat, CM enzyme, EKG, EEG, chest fluoroscopic examination.

对276例脑器质性精神障碍患者进行12周的奥氮平治疗，使用BPRS、TESS评定治疗效果，治疗前后查血常规、尿常规、肝功能、肾功能、血糖、血脂、心肌酶、心电图、脑电图、胸透检查。

Methods:(1) Silica column chromatography and LH-20 were used in phytochemistry study.(2) Based on the extraction ratio of arctiin, the best extraction procedure was acheived under the direction of L9(34) orthogonal experimental design. From 10 types of resins, the one with the maximal binding ability for Arctii was selected and silica-column choramatograpy was used in its seperation and purification procedure.(3) The animal diabetic experimental model in Wistar rats was established with streptozotocin and many biochemical factors were used to indicate the pharmacological effects of arctiin, including glycosylated hemoglobin, serum creatinine, seralbumin, monoxide nitrogen, glycerin trilaurate, cholesterol, HDL-ch, LDL-ch, Blood urea nitrogen, creatinine, SOD, ET, MDA, total Urine protein and albuminuria.

(1)采用硅胶柱层析、聚酰胺层析、LH-20等方法对牛蒡子的化学成分进行研究；(2)采用正交实验设计方法，以牛蒡子苷为指标，确定了牛蒡子乙醇回流提取的最佳工艺；对10种类型大孔吸附树脂进行筛选，筛选出对牛蒡子苷吸附效果最佳的树脂；采用硅胶柱层析法，对牛蒡子苷进一步分离纯化；(3)建立实验性糖尿病大鼠模型，以GLU、TC、TG、HDL-ch、LDL-ch、TB、ALB、BUN、CREA、GHbAlc、NO、SOD、ET、MDA和尿液中TB、ALB为指标，研究牛蒡子苷药理活性；(4)以差速消化法纯化Wistar大鼠的主动脉内皮细胞并进行传代培养，测定牛蒡子苷对高糖条件下RAECs活性、RAECs释放LDH、MDA和NO的变化以及RAECs表达内皮型一氧化氮合酶表达的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。