氮化法

The methods determing the groups in the modified starch have been developed, that is acidimetry for the basicity of acidified starch, complexing titration for the substitution degree of carboxymethyl starch, and heat paste titration and carbonyl process respectively for the carboxyl and nitrogen of oxidized starch.

建立了以酸碱滴定测定酸化淀粉的碱量、络合滴定法测定羧甲基淀粉的取代度、热糊滴定和羟胺法测定氧化淀粉中羧基及羰基、消化定氮测定阳离子淀粉等几种变性淀粉的基团分析方法。

Methods Immunohistochemical SP method was used to detect the eNOS and iNOS gene expression in the tissues of 24 cases of choriocarci-noma and 22 cases of normal chorion of early pregnancy.

用免疫组化SP法，对24例绒毛膜癌和22例早孕正常绒毛组织中内皮型一氧化氮合酶及诱导型一氧化氮合酶进行检测。

A series of novel dendrons with 1,3,5-triazine structure were synthesized by convergent strategy using cyanuric chloride, p-hydroxyazobenzene,β-naphthol, 1-hydroxyanthraquinone, 3,5-dihydroxybenzyl. They were the first generation p-phenyl-azo-phenyloxy-terminated dendron G1.0, the first generation naphthyloxy-terminated dendron G1.0, the first generation naphthyloxy-terminated and 1-anthraquinonoxy-cored dendron G, and the second generation naphthyloxy-terminated dendron G2.0.

以对羟基偶氮苯、β-萘酚为端基，三聚氯氰、3，5-二羟基苄醇为支化单体，1-羟基蒽醌为核心，用收敛法合成了一系列新型结构的含均三嗪环的树枝块：第一代端基为偶氮苯的树枝块G1.0、端基为萘氧基的树枝块G1.0、端基为萘氧基、中心为蒽醌的树枝块G、第二代端基为萘氧基的树枝块G2.0。

Methods: Separate and abstract the effective components of Huangqi (Omni-Saponin、total-flavone) and Danshen (Danshen-ketone、 Danshen-Quinone). Applying L-Arginine methyl ester to pregnant rats by I. P to make Nitrogen Oxide Synthesis blocking models (uterus-placenta-fetus lacking blood and oxygen model or pregnant hypertension syndrome model). On day 12, 18 of pregnancy paunched the rats and took out the fetus and placenta/uterus. Measured the basal BP during pregnant period and BP、protein、basal activity each day. Observed placenta/deciduas ultramicroscopic structure by electron microscope and took count of languishment cells. Measured the expressive intensity of bcl-2、Bax of placental nutrient cells by SABC method.

一氧化氮合成酶阻滞剂L-精氨酸甲酯妊娠大鼠腹腔给药，制作一氧化氮合成阻滞模型（子宫-胎盘-胎儿缺血缺氧或妊娠高血压模型），妊娠第12、18天剖腹取胎、胎盘／子宫，妊娠期测基础血压，每日测血压、蛋白质量、基本生理活动；电镜观察胎盘／蜕膜超微形态结构，并进行细胞凋亡计数；免疫组化检测胎盘滋养细胞bcl-2、Bax表达强度；原位杂交法检测胎盘滋养细胞、血管内皮细胞VEGF、eNOS、iNOS、MCP-1、MMP9（基质金属蛋白酶9）mRNA表达。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope .

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH-d的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因c-fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope.

为了证实穹窿下器在脑内免疫调节系统中的作用，在本研究当中，应用了NADPH刁的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术，来研究在腹腔内给予大肠杆菌内毒素后，脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因（immediate early genes沁－fos的表达与星形胶质细胞之间的关系，以及穹窿下器与其它脑区相比较时的特异性改变。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

Described in chapter 3 is the immediate application of the Clemmensen reductive rearrangement reaction to the polycyclic system as illustrated in a elaboration of the 5-6-7 tricyclie core skeleton of Stemona alkaloids in 5 steps: N-alkylation, [3, 2] -sigmatropic rearrangement, Dieckmann condensation, oxidative cyclization, reductive rearrangement, starting from ethyl pipecolinate hydrochloride.

我们以哌啶酸乙酯为起始原料，经氮烷基化，[3，2]-σ重排，Dieckmann缩合，自由基环化，6-6-6元环系到5-6-7元环系的还原重排五步反应，高收率地构筑了百部生物碱的5-6-7氮杂三环骨架，并用X射线晶体衍射法确定了其结构。

Four main saccharifying technical conditions of wheat beer influencing α-amino nitrogen content of wort including wheat malt proportiong, the ratio of water and raw materials, holding time at 52 ℃, and holding time at 65 ℃ were optimized by response surface method and the mathematical model of each factor incluencing α-amino nitrogen content was established.

采用响应曲面分析法对影响小麦啤酒麦汁α-氨基氮含量的4个主要糖化工艺参数即小麦芽比例、水料比、52 ℃保温时间和65 ℃保温时间进行优化，建立了各因素对麦汁α-氨基氮含量影响的数学模型。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。