氮化法

The tea polyphenols have been determined by spectrophotometric analysis using the principle of colored compounds produced by the diazonium coupled reaction.

利用芳香族伯胺重氮化偶合生成有色化合物的原理，建立了分光光度法测定茶多酚含量的方法。

Spetrophotometry ; determination/tea polyphenols ; diazonium coupled reaction

分光光度法；测定/茶多酚；重氮化-偶合反应

This method is based on the diazotization of sulfadiazine with sodium nitrite in acidic medium and the coupling reaction with α-naphthylamine.

在盐酸介质中，利用磺胺嘧啶－亚硝酸钠－α-萘胺的重氮化－偶合反应，建立了流动注射光度法测定磺胺嘧啶的新方法。

Use nitrite and the Sulfanilic acid diazotization in acidic conditions,with dinitroaniline coupling formation color reaction,established the method of using the color reaction Determination Chinese cabbage s Nitrite content.

在酸性条件下亚硝酸盐与对氨基苯磺酸重氮化后，再与盐酸萘乙二胺偶合形成颜色反应，建立了用比色法测定大白菜中亚硝酸盐的方法。

The method is based on the diazo-reaction between nitrite ion and fuchsin acid in a hydrochloric acid medium.

研究了在酸性介质中，NO2-与酸性品红发生重氮化反应，致使酸性品红的荧光强度随NO2-加入量增加而明显降低的现象，建立了荧光熄灭法测定痕量NO2-新方法。

Methods The changes of NOS neuron of mouse brain in proconditioning to hypoxia were detected by histochemistry method.

采用组化法测定小鼠脑内一氧化氮合酶神经元在缺氧(1次缺氧，4次缺氧)预适应中的变化。

A rapid and sensitive flow-injection spectrophotometric method was proposed for the determination of metoclopramide. This method was based on the diazotisation of metoclopramide with sodium nitrite in acidic medium and coupling with α-naphthylamine.

基于在盐酸介质中，利用甲氧氯普胺一亚硝酸钠-α-萘胺的重氮化-偶合反应，建立了流动注射光度法测定甲氧氯普胺的新方法。

Methods Rabbits were infected with cercaria of S. japonicum percutaneously. The SP immunohistochemical technique was used to analyse the expression and localization of cNOS and iNOS in the rectums of normal and schistosoma rabbits.

采用腹部敷贴法建立血吸虫病兔模型，SP 焦虑免疫组化法分析结构型和诱导型一氧化氮合成酶在正常兔和血吸虫病兔直肠壁的表达与分布。

To study the technology used for brewing bifidobeer fermented by Bifidobacterium bifidum and Saccharomyces cerevisiae,Australia malt is sprinkled and milled.Wort is prepared by lixiviated saccharification,filtered,hops added,boiled and co-fermented by Bifidobacterium bifutum and Saccharomyces cerevisiae,and followed by antisepsis and centrifugation.

采用增湿法粉碎澳大利亚麦芽，麦皮破而不碎，麦粉较细，干法粉碎大米成细粉；选用双醪浸出糖化法进行麦汁制备、过滤、添加酒花和麦汁煮沸，麦汁呈黄色、澄清，可发酵糖和α-氨基氮的含量符合发酵要求；利用双歧杆菌和酿酒酵母共同发酵啤酒，消毒灭菌和低温离心后进行灌装；通过几项重要指标的检测，进行评判啤酒的质量和性能。

METHODS Dinitrofluorobenzene was used to induce the contact dermatitis in mouse ear, rhIL-11 0.375-1.5 mgkg^(-1)d^(-1) sc for 10 d, the degree of skin inflammatory reaction was observed. At the same time, the serum level of tumor necrosis factor-α and interleukin-6(IL-6) was measured by radioimmunoassay; the serum level of nitrogen monoxidum was detected by biochemical method and the expression of intercellular adhesion molecule-1(ICAM-1) was detected by immunohistochemical method.

采用2，4-二硝基氟苯致小鼠耳部皮肤接触性皮炎试验模型，观察sc rhIL-11 0.375~1.5mgkg^(-1)d^(-1)，连续10d后皮肤炎症反应程度，同时采用放射免疫分析法测定血清中肿瘤坏死因子-α和白细胞介素-6(IL-6)的水平，采用生物化学检测法测定血清一氧化氮的水平，采用免疫组化法测定皮肤中细胞间粘附分子(ICAM-1)的表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。