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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

On days 14, serum proteins and amino acids concentration and peripheral lymphocyte blastogenesis were determined. At the end of the study, the animals were sacrificed, hydroxyproline and tensile strength of wound, and intestinal mucosal DNA, RNA and protein content were examined.

测定饲料摄入量、体重、氮平衡、血浆蛋白和氨基酸含量、外周血淋巴细胞转化率、伤口埋植海绵中羟脯氨酸含量、皮肤抗张力强度和小肠粘膜蛋白质及核酸。

Rapid-fire like I'm blastin a Tec, never jam though Never get high, never run out of ammo Niggaz hatin and shit cause I slayed your bitch You know your favorite, I know it made you sick And now you're, actin raw but you never had war Don't know how to carry your hoe, wanna marry your hoe Now she's mad at me, causer Your Majesty Just happened to be a pimp What a tragedy She wanted, us to end, cause I fucked with friends She gave me one more chance and I fucked her again I seen her tears as she busted in, I said, Shit..

速射一样,我blastin 1 TEC的,没有干扰,但从未获得高的,从来没有失控血氨 niggaz hatin和shit的事业i slayed你的女巫,你知道你最喜欢的,我知道这令你生病现在您,肌原,但你从未有过的战争不知道如何开展你的锄头,想嫁给你的锄头现在,她的疯狂,在我来说,致女王陛下偏偏是一个皮条客什么是悲剧,她想,我们能够结束,事业i fucked与好友她给了我一个机会,我fucked她再次 i看到她的眼泪,因为她逮捕归案,我说: shit的。。

Without prompt intervention, hyperammonemia and the accumulation of other toxic metabolites (e.g., glutamine) result in ICP, increased neuromuscular tone, spasticity, ankle clonus, seizures, loss of consciousness, and death.

不及时干预的话,血氨过多和其它有毒代谢物的累积导致颅内压、神经肌肉紧张加强、强直、脚踝阵挛、癫痫、失去意识和死亡。

The- cytogenesis of AI and A II cells in non-hepatic and non-hyperarnmonemic encophalopathy was discussed.

讨论了非高血氨性脑病中AⅠ和AⅡ细胞的形成机理。

Objective To explore effects of glutamine on immune function ,plasma endotoxin level of the rectum cancer patients.

目的 探讨谷氨酰胺二肽对直肠癌术后患者免疫功能和内毒素血症的影响。

Objective To investigate the association between hyperhomocysteinemia and deep thrombosis of lower extremities.

目的 探讨高同型半胱氨酸血症与下肢深静脉血栓形成之间的关系。

Rabbits received naloxone HCl intracerebroventricularlyor naloxone methiodide IV, a quaternary compound that does notcross the blood–brain barrier, 5 min before FENT treatment(5 or 50 g/kg, IV).

使用 FENT 治疗(5或50 g/kg ,静脉注射)前5min ,向家兔脑室内注射盐酸纳洛酮或静脉注射甲硫丁氨酸纳洛酮(一种四元化合物,不能透过血脑屏障)。

Objective To evaluate the effects of tranexamic acid on coagulation and fibrinolysis in patients undergoing thoracotomy.

目的评价氨甲环酸在开胸手术中的止血作用和对开胸手术围术期凝血纤溶功能的影响。

Endotoxin and tetrahydrobiopterin enhanced the activation of NO synthesis and ACO, but L-arginine, the precursor of NO, showed noticeable inhibition on the elevation of NO metabolites and the degree of ACO. In addition, L-arginine show prominent inhibition on myocardial fibrinosis.

诱导iNOS活性的药物也可加重动脉钙超负荷损害,然而NO合成的前体物质L-精氨酸对ACO早期血NO〓〓/NO〓〓升高有明显抑制,同时抑制ACO,尤其对心脏钙超负荷损害有明显的保护作用。

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