氨脂质

To investigate the practicability of galactosylceramide serving as new targeting delivery carrier for antiHIV, azidothymidine was encapsulated in liposomes containing GalCer and the pharmacokinetics and tissue distribution of AZT-GalCer and free AZT were observed in mice.

本文采用半乳糖神经酰氨(Galactosyceramide，GalCer)制备了齐多夫啶(Azidothymidine，AZT)半乳糖神经酰氨脂质体并观察AZT-GalCer及游离AZT在小鼠体内的药物动力学过程和组织分布，探讨AZT-GalCer作为治疗AIDS靶向新载体的可行性。

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的：探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

OBJECTIVE: To investigate transfection and expression of liposome-mediated cytosine deaminase gene in rabbit BMSCs.

目的：观察脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其表达。

Results: The tHey and blood fat level in blood plasma and expression of MCP-1mRNAof aorta on model group is obviously higher than that of control group (P＜0.05);the result of immunohistochemistry shows that the expression of VCAM-1 and MMP-2enhanced, Bcl-2 is lower; aorta slice shows endarterium discontinuation, strippingpartly, smooth muscle cell arrange puffly and chaotic, infiltration of lipoid ofdifferent degree are observed in aorta wall, pathological changes suffusion, morexanthoma cell, plaque protrude lumens. The they and blood fat level in blood plasmaand expression of MCP-lmRNA, VCAM-1, MMP-2 of aorta on intervention group aremuch lower than that of model group (P＜0.05); Bcl-2 of intervention group isstrengthened, and the pathomorphology and immunohistochemistry of aorta showpathological changes are slighter than model group.

结果：模型组，血浆中同型半胱氨酸浓度、血脂水平、主动脉MCP-1mRNA表达显著高于正常对照组(P＜0.05)，免疫组化示主动脉内皮细胞VCAM-1、MMP-2表达增强，Bcl-2表达降低；主动脉切片发现动脉内膜不连续，部分剥脱，平滑肌细胞排列疏松、紊乱，主动脉壁均见到程度不同的脂质浸润，其病变弥漫，泡沫细胞较多，可见斑块突出管腔；干预组，血浆中同型半胱氨酸含量、血脂水平显著低于对照组和模型组(P＜0.05)，主动脉MCP-1mRNA、VCAM-1、MMP-2表达显著弱于对照组和模型组(P＜0.05)，Bcl-2表达增强(P＜0.05)，主动脉病理形态学和免疫组织化学检测示病变轻于模型组。

AIM: To explore the effect of homocysteine-mediated endoplasmic reticulum stress on lipid metabolism in hepatocytes.

目的：探讨同型半胱氨酸介导的内质网应激对肝细胞脂质代谢的影响。

Objective To synthesize new cationic liposome cytofectin N 4 spermine cholesteryl carbamate as a gene delivery vehicle for gene therapy of lung diseases.

目的 合成新型阳离子脂质体细胞转染素N4 精氨胆固醇羰酰氨，作为基因载体，用于肺部疾病的基因治疗。

The activity of superoxide dismutase in the myocardial cells was determined by the diphenol method, plasma malondialdehyde content was measured by TBA assay, the culture medium LDH content was measured by the acetonic acid method, the intracellular calcium concentration was measured by fluorescence spectropho- tometer- The results was shown that aspartate-K.

结果显示，门冬氨酸钾镁能减少阿霉素中毒心肌细胞乳酸脱氢酶的释放量，降低细胞内丙二醛含量及游离钙浓度，同时可升高细胞内超氧化物歧化酶活力；结果表明，门冬氨酸钾镁可能通过抗脂质过氧化及逆转细胞内钙离子过负荷作用进而保护中毒心肌细胞。

Methods 60 female mice were randomly divided into 4 groups (n=15):Mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of D-galactose[1000 mg/(kg1·d)] for 42 d to establish subacute aging model.

60只雌性昆明种小鼠，随机分为4组(n=15)：正常对照组皮下注射生理盐水；其余3组每日颈背部皮下注射 D-半乳糖[1000 mg/] 造成小鼠衰老模型，同时各组分别灌胃生理盐水、枸杞多糖[200 mg/]、丹参酮[1 500 mg/]。42 d后，测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose［1000 mg/(kg1·d)］ for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides ［200 mg／group,tanshinone ［1 500 mg／］ group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

目的 比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法 60只雌性昆明种小鼠，随机分为4组(n=15)：正常对照组皮下注射生理盐水；其余3组每日颈背部皮下注射 d-半乳糖［1000 mg/］造成小鼠衰老模型，同时各组分别灌胃生理盐水、枸杞多糖［200 mg/］、丹参酮［1 500 mg/］。42 d后，测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

The results show that the hyperthyroidism group has elevated levels of choline, glucose and declined levels of VLDL, LDL, cholesterol, lactate, glycoprotein and alanine in serum samples relative to the healthy group. In urine samples, the hyperthyroidism group show increased levels of glucose, citrate, taurine and creatinine as well as decreased levels of hippurate, trimethylamine-N-oxide, formate and succinate.

结果表明，甲亢组血清中的胆碱、葡萄糖和三甲胺等物质的含量升高，而VLDL，LDL和胆固醇等脂质以及乳酸、糖蛋白和丙氨酸等代谢物的含量下降；甲亢组尿液中的葡萄糖、柠檬酸、牛磺酸以及肌氨酸等代谢物的含量升高，而马尿酸、TMAO、甲酸和琥珀酸等代谢物的含量下降。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。