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The changes of nutrition in milk treated with different technologies are also determined. Results showed that, 1 higher pressure, longer holding time and more alternative times favour a larger destruction of microorganisms; 2 the combined effect of hurdle technology and pressure on the destruction of microorganisms in fresh raw milk is more effective than only using pressure treatment, among which the effect of ε-polylysine on inactivation of microorganism in fresh raw milk is more effective than that of Nisin with the same concentration of 100mg/L; 3 the whole proteins in milk treated by the different types of sterilization have been detected by SDS-PAGE electrophoresis, and comparing with the whole proteins' electrophoretogram of fresh raw milk, we found that the first spectrum band near the negative pole was disappeared and the colours of the second spectrum band was tint in UHT, pasteurism and 500MPa treated milks, which was suggested that some proteins' subunits were dissociated, but the 400MPa treated milk showed the similar eletrophoretogram with the fresh raw milk; 4 the loss of threonine, VA and VC in high pressure treated milk was more less than those in pasteurism treated milk, in particular threonine and VA were almost retained, and the contents of VB1 and VB2 in the former are also more than that in the latter; 5 high pressure treated milk had longer shelf life than pasteurism treated milk, extending the sale semidiameter of milk.

压力越高，保压时间越长，交变次数越多，杀菌效果越好；2）采用栅栏技术对牛乳中的微生物具有更有效的杀菌作用，其中相同浓度的（终浓度100mg/L）ε-聚赖氨酸较Nisin具有更强的杀菌效果；3）利用SDS-PAGE电泳研究了不同杀菌方式对牛乳总蛋白的影响，发现经UHT、巴氏和500MPa交变处理的牛乳与生鲜牛乳相比其总蛋白电泳图谱靠近负极方向第一条谱带消失，第二条谱带颜色变浅，由此推测经上述处理后牛乳中有一些蛋白质的亚基发生了解离，而经400MPa交变处理的牛乳与生鲜牛乳的总蛋白电泳图谱相似；4）超高压牛乳营养成分与生鲜牛乳相比，苏氨酸、VA、VC在超高压处理中受到的破坏明显比巴氏杀菌小得多，特别是苏氨酸和VA几乎完全被保留下来，而VC含量也要比巴氏奶高出25%以上，对于VB1、VB2，检测数据也表明，超高压灭菌对其破坏要比巴氏灭菌小，其含量分别比巴氏杀菌牛乳高出9.5%和2.6%；5）与巴氏杀菌乳相比，超高压杀菌有助于延长牛乳的保质期，扩大牛乳的销售半径。

The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示：先证者尿检呈强阳性；其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变，为半合子突变，而其母、其姐为杂合突变；正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变；不同物种氨基酸序列的同源性比对显示： c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守；酶活性测定的结果显示：先证者的IDS酶活性仅为2.3 nmol/4 h/mL，大大低于正常值，而其父的为641.9 nmol/4 h/mL，其母的血浆酶活性为95.8 nmol/ 4h/mL，其姐的为103.2 nmol/4 h/mL。

Pereme , separately are 209%,295%,285%; the increasing of accumulating amount separately are 209%,295%,285%; The use of silicon has a tread to rise dry weight of Bermudagrass ,Agrostis Lolium. Pereme ; Under the drought condition , the use of silicon suppressed the reducing of the relative water content of leaf in turfgrass , delaying the time of wither , stress for the 11th day in the drought .compared with water normally and not using silicon ,after use silicon, the RWC of Bermudagrass , Agrostis Lolium. Pereme only separately depressed 14%,16%,32%; Under the drought condition ,the use of silicon can increased the content of proline of Bermudagrass ,Agrostis Lolium. Pereme, stress for the 11th day in the drought and using silicon , compared with water normally and ueing silicon, the content of Pro separately increased 203%,199%,473%;The using of silicon depressing the ET of Bermudagrass and Lolium. Pereme.Under water normally condition, separately depressed 21%,8% at eleven days ;Under drought condition , separately depressed 17%,5%.

施硅增加了草坪草狗牙根、剪股颖和黑麦草的含硅量，分别为209％、295％、285％；积累量分别提高了240％、364％、355％；施硅对草坪草狗牙根、剪股颖和黑麦草的干物质积累有促进趋势；在干旱的条件下，施硅抑制了草坪草的叶相对含水量的降低，延迟了叶片萎焉的时间，在干旱胁华中农业大学2001级时一艳丽硕士学位论文迫第n天，与正CK相比，施硅后狗牙根、剪股颖和黑麦草分别只降低了 14%；16%，32%。；施硅增加了狗牙根、剪股颖、黑麦草在干旱条件下脯氨酸的含量，增强了渗透调节能力，在干旱施硅的条件下，与正51相比，在干旱胁迫第11天，狗牙根、剪股颖和黑麦草脯氨酸的含量分别增加了2叨%、199%、473%；施硅减少了狗牙根和黑麦草的蒸散量在正常浇水的条件下，与正CK相比，第11天狗牙根和黑麦草的蒸散量分别降低了21%、吕%；在干旱的条件下，与干CK相比，在干旱胁迫的第11天，狗牙根和黑麦草的蒸散量分别降低了17%、5%。

We make chloroacetylaion to N-terminal of Asp, and then convert this compound to GD dipeptide by aminolysis. Followed the lipase -catalyzed peptide synthesis of RGD in aqueous water-miscible organic cosolvent is studied. We set up a new efficient reaction method to synthesize RGD tripeptide. The problem that peptide containing polar amino acid can not be synthesized readily in organic solvent was solved. We offer a new experimental proof for PPL-catalyzed peptide synthesis and make a more general analysis for the effect of some factors in theory.

采用氯乙酰基保护天冬氨酸，然后进行氨解生成GD二肽，利用猪胰脂肪酶催化合成RGD三肽，着重解决了亲水氨基酸/肽片段底物的溶解性与合成产率低的问题，建立了一种新的有效合成RGD三肽的反应模型，并探索解决了极性较强的氨基酸的酶促肽合成的问题，对脂肪酶催化合成肽的影响因素从理论上作了较全面的分析，为肽片段的缩合反应提供了一种新思路。

With aggravation of drought stress, ZXY04P-75 and ZXY04P-201 increased in proline content while that of ZXY03P-173 and ZXY04P-239 increased initially, reaching the peak at the 8th day, then declined. And the rate of net photosynthetic declined notably with the intensity of drought stress, The minimum of decline rate of net photosynthetic is ZXY04P-201. 4. The results of photosynthetic mechanism for 4 Dactylis glomerata L. show: the net photosynthetic rate, transpiration rate,stomatal conductance and chlorophyll decreased dramatically, Compared with weak drought resistance ZXY04P-239,the net photosynthetic rate, transpiration rate, stomatal conductance and chlorophyll in the leaves strong drought-resistance ZXY04P-201 decreased slowly.

连续水分胁迫及复水条件下4份鸭茅抗旱机理的研究表明，叶片水分饱和亏缺随着干旱胁迫强度的增加而增加，其中变化幅度最大的是ZXY04P-239，最小的是ZXY04P-201；ZXY03P-173和ZXY04-201质膜相对透性和可溶性糖含量随着干旱胁迫强度的增加而增加，而ZXY04P-75和ZXY04P-239出现单峰变化，在胁迫第8d后达到最大，然后下降；ZXY03P-173和ZXY04P-239脯氨酸含量在干旱胁迫第8d达到最大，而ZXY04P-75和ZXY04P-201的脯氨酸含量随着干旱胁迫程度的加深而逐渐达到最大；4份材料叶片的净光合速率随着干旱胁迫强度的增加而减小，其中ZXY04P-201的净光合速率下降率胁迫至12d时为最小。

Experimental results showed that, in this process, the optimal pH of the shortcut nitrification was 8.0～8.5; when the pH was less than 6,the nitrification is completely inhibited; when the pH was 6～7, at the end of aeration, the Nitrobacteria become the dominant bacteria because of the dropping of the free ammonia concentration. It is known to all, the activity of the Nitrobacteria and Nitrosomonas was affected by FA concentration, so nitrification was affected by FA concentration. According to the author's research results, the FA concentration which inhibit the Nitrobacteria was below 1.0mg/L,which inhibit the Nitrosomonas was above 10mg/L.

实验研究结果表明：本工艺中，短程硝化反应的最佳pH值在8.0～8.5之间，当pH小于6时，整个硝化反应都会受到抑制，当pH在6～7之间时，随着游离氨浓度的降低，在曝气后期，硝化菌将会占据优势；FA浓度对短程硝化反应的影响是通过对亚硝酸菌和硝酸菌的抑制来实现的，在本研究条件下，游离氨对亚硝酸菌的抑制浓度在10mg/L以上，对硝酸菌的抑制浓度在1.0mg/L以下；温度对短程硝化反应的影响是双重的，既影响微生物的生理活性，又影响FA浓度。

To reduce the poisonous compounds in the breeding ponds,five species of bacteria including denitrobacteria ,photosynthetic bacteria,thiobacteria and bioflocculation bacteria were screened from the breeding water.

针对养殖水体中因氨态氮、硫化氢和小分子有机酸富营养化引起的污染问题，分离筛选出硝化细菌、反硝化细菌、光合细菌、硫化细菌和生物絮凝菌等具有不同生理功能的污染物治理菌株，经优化配伍制备出性能优良的复合功能菌，结果表明：硝化细菌对氨态氮的去除率达97.8%，亚硝态氮的去除率达95.7%，反硝化细菌对硝态氮的去除率为96.4%，光合细菌和硫化细菌对硫化氢的去除率为55%，微生物絮凝菌的絮凝效率为83%；复合功能菌对CODCr、NH4+-N，总氮、硫化物的去除率分别可达94.3%，89.6%，88.7%和71.3%。

Finally, the pH value, lactic acid, water soluble carbohydrate, ammonia nitrogen and cellulose contents were determined for WH silage prepared under the suitable adding doses of lactobacillus and cellulase at day 1, 3, 5, 15, 25, 35 after ensilaging. The pH value decreased from 5.5 before ensiling to 4.3 on the 35th ensiling day, whereas the reverse change tendence was observed for lactic acid content. The cellulose content of silage was lower from 26.5%DM in raw material to 22.3%DM.

结果表明：pH值由青贮前的5.5至青贮第35天时降至稳定的状态，而乳酸含量的变化趋势相反；凤眼莲茎叶纤维素含量从未青贮前的26.5%干物质，至青贮第35天降至22.3%干物质，降低了约4个百分点；青贮料中可溶性碳水化合物的含量随青贮时间的延长而逐渐下降，但是在第5天到第15天的时间段里，可溶性碳水化合物的含量无显著性下降；氨态氮占总氮的比例从青贮第1天的4.61%显著上升至第5天的8.25%，而从第5天开始到第35天，氨态氮占总氮的比例上升的幅度较小。

Through the research of this paper, a conclusion can be drew: NaCl treatment raise the NH4＋-N removal ratio obviously by 18%, and is better than treating by heating, H2SO4, HCl and NaOH. The most reasonable concentration of NaCl is 0.8~1.0mol/L considering technical, economic and other factors synthetically; The best conditions of NaCl modifying is as follows : temperature 70~75℃, time 2.5hours, granularity ＜120mesh, volume/mass 25ml/g; As additive forming pores, activate carbon powder is a little better than charcoal powder, but the effect of both is much better than sawdust.

通过本论文的研究得出以下结论：NaCl改性能明显提高沸石对氨氮的去除效率，氨氮去除率提高了18%以上，其改性效果优于加热、H2SO4、HCl、NaOH等处理方法，综合考虑技术经济等因素的影响，取0.8～1.0mol/L为最合理的改性浓度；NaCl改性的最佳条件如下：温度70～75℃，时间2.5h，沸石粒径＜120目，体积质量比25ml/g；作为微孔沸石的造孔剂，活性炭粉略优于木炭粉，但二者的效果均比木屑好得多。

Firstly, phenyliodine diacetate was immobilized to the 2% cross-linked polystyrene or the liner polystyrene resin. The more iodine the resin had, the lower conversion to phenyliodine diacetate. We made our endeavor to extend the range of useful application of polystyrene supported phenyliodine diacetate in organic synthesis:(1) the reagent was used as an effective reagent of carbonyl regeneration from oximes and semicarbazones of ketones.(2) the reagent was also applied to the synthesis of 1, 3, 4-oxadiazoles, 1, 3-disubstituted-2-pyrazoline, 2-arylbenzoxazole from aldehyde N-acylhydrazones, aldehyde hydoazones and phenolic Schiff's bases respectively.

首先，我们以2％交联的聚苯乙烯和线性的聚苯乙烯树脂为载体，制备了聚合物负载的二醋酸碘苯，通过研究发现：(1)聚苯乙烯二醋酸碘苯是酮肟、缩氨脲有效的去保护试剂，不仅芳香酮，脂肪酮和α，β不饱和酮的肟、缩氨脲也能顺利转化为相应的酮，实现酮基的再生；(2)我们还利用此试剂，分别与醛的N-酰基苯、N-酰基苯腙及邻羟基芳亚胺反应，在温和的条件下发生成环反应，以良好的产率生成1，3，4-噁二唑、吡唑啉和二取代苯并噁唑等杂环化合物；(3)聚苯乙烯二醋酸碘苯还可有效地将邻羟基芳酮的酰腙经氧化、重排成1，2-二酰基苯。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。