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The holding potential was -70mV.(2) By whole-cell voltage-clamp technique, after slices were preincubated with glycine receptor competitive antagonist alone or with GABA〓 receptor competitive antagonist alone or with GABA〓 receptor incompetitive antagonist alone or with GABA〓 receptor and glycine receptor competitive antagonists collaterally or with GABA〓 receptor incompetitive antagonist and glycine receptor competitive antagonist collaterally, the effects of 100μM propofol on EPSCs of the CA1 neurons of the rat hippocampus were investigated. The holding potential was -70mV.

2应用全细胞膜片钳技术,观察100M丙泊酚对单纯用甘氨酸受体竞争性拮抗剂预孵、单纯用GABA〓受体竞争性拮抗剂预孵、单纯用GABA〓受体非竞争性拮抗剂预孵、合用GABA〓受体和甘氨酸受体竞争性拮抗剂预孵以及合用GABA〓受体非竞争性拮抗剂和甘氨酸受体竞争性拮抗剂预孵的大鼠海马脑片CA1区电刺激诱发的EPSC的影响。

The Nterminal cyclization of peptides, as one of a variety of modification introduced in sample preparation, has been preliminarily studied in this work.

目前,肽段鉴定时常用的添加残基修饰有甲硫氨酸的氧化和半胱氨酸的氨乙酰化,而对其它修饰研究很少。

As a result, both the detection limit of the sensor decreased by 2~3 magnitudes and the signal magnitude increased about ten times than that of fly labellar single hairs. The sensitivity to many stimulants from simple molecules such as NaCl, KCl, sucrose, glycine, proline, glutamic acid and aspatic acid to macromolecule such as bovine surem albumin was lower than 10〓mol L〓. For this reason, the maxillary palp of cockroach is reveled as an important candidate for construction of a new type of intact-receptor biosensors.

这种协同作用导致下颚须感觉区对有些刺激物如NaCl,KCl,蔗糖,甘氨酸,脯氨酸,谷氨酸,天冬氨酸,牛血清白蛋白的响应阈值比一般单根感觉毛至少低2-3个数量级,达10〓molL〓以下;信号幅度提高约十倍,是一种新型的适合用作传感器的敏感材料。

The major route of β-alanine production for pantothenate in Escherichia coli is through the α-decarboxylation of L-aspartate, which is catalysed by the enzyme L-aspartate-α-decarboxylase. The panD mutant of E. coli is β-alanine auxotrophy.

在大肠杆菌中,用于泛酸生物合成的的β-丙氨酸主要是由L-天冬氨酸α位脱羧而产生,这个生化反应由panD基因编码的L-天冬氨酸-α-脱羧酶催化。

The purpose of this paper is to synthesize some phosphonium salts with PPh3 and some chiral quaternary ammonium salts with L-Valine. And we also use L-Valine and L-Phe-OMe to synthesize a new type catalyst —2,5-diketopiperazine , the simplest compound of cyclo-peptide.

本文利用廉价易得的三苯基磷和手性L-缬氨酸作为起始原料,合成了几种普通季鏻盐和手性季铵盐,并且利用L-缬氨酸与L-苯丙氨酸甲酯反应,合成了一种新型的相转移催化剂——2,5-二酮哌嗪环类的化合物。

As examples, The apparent binding constants of zinc ion with cysteine and tartaric acid system, and zinc with cysteine and lactic acid system have been obtained.

研究了三元相互竞争体系的毛细管电泳测定方法,测定了金属锌离子与酒石酸和半胱氨酸以及锌离于与乳酸和半胱氨酸两个三元结合体系的结合常数,并以此比较了两种锌形态与半胱氨酸结合能力的大小。

Removal of high concentration of ammoniacal nitrogen discharged in rare earth production process by VMD is basically studied in this paper, and VMD can achieve the purpose of ammoniacal nitrogen removal and reuse of recovered ammonia at the same time.

进行了减压膜蒸馏法处理含高浓度氨氮的稀土冶金所排废水的基础研究,该法可达到脱除废水中高浓度氨氮及回收氨回用于生产过程的目的。

The content of NH3-N of Coniferous mixed forest is higher than rainfall and other forest. The content of NH3-N of Corylus cornuta marsh is lowest. The purgative effect of NH3-N of Larix gmelinii plantation is better too.

樟落混交林的氨氮含量明显高于大气降水和其它林分类型,榛子灌丛的氨氮含量最低,落叶松人工林对氨氮的净化效果也很好。

The partitioning behavior and the influence of five amino acids(glutamic acid, cysteine, phenylalamine, lysine and arginine)in aqueous twophase systems of ethanol and dipotassium hydrogen phosphate have been studied.

研究了5种结构和性质各异的氨基酸(谷氨酸,半胱氨酸,苯丙氨酸,赖氨酸,精氨酸)在亲水性有机溶剂乙醇和磷酸盐溶液形成的乙醇/K2HPO4双水相体系中的分配行为及其影响因素。

Chinensis and Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible NOS activity also decreased significantly with the time elapsed in WSSV infected shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization test showed that WSSV of the two penaeid species couldn't be detected until iNOS started decreasing.

在此基础上,我们建立了对虾血细胞中诱导型一氧化氮合成酶的鉴定方法—硝基四氮唑蓝还原法和血细胞形态法以及酶活力的测定方法—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件,结果显示:对虾血细胞在4℃下与L-精氨酸和LPS孵育8h以及当L-精氨酸浓度为2.5mmol l〓LPS浓度为100μg ml〓,Ca〓浓度为2.5mmol l〓时,iNOS活力的测定结果最佳。

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