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Then the intracellular calcium concentration of neurons was measured by laser confocal scanning microscope , represented by the mean intracellular fluorescent intensity. On the ice, the cerebral cortical tissue at the infarction margin of the section after optic chiasm was rapidly dissociated about 100mg, then made into brain homogenate in low temperature. The high performance liquid chromatography was used to determine the Glu, Asp and Gly contents in homogenized brain tissue.

视交叉平面用2%TTC染色,用图像处理软件计算出脑梗死面积占该片脑组织面积的百分比;取视交叉前平面梗死边缘区皮层组织约100mg,用酶消化法制作神经细胞悬液,用激光扫描共聚焦显微镜检测皮层神经元细胞内游离钙离子浓渡,以细胞平均的荧光强度代表细胞内钙浓度;冰上迅速分离视交叉后梗死边缘区皮层组织约100mg,于低温下制作脑组织匀浆,用高效液相色谱法测定皮层匀浆中谷氨酸、天门冬氨酸、甘氨酸的含量。

The optimum pH is 8-10 for hypha growth and 7-9 for conidiospore germination; The hypha could not grow on the culture medium which nitrogen source comes from urea and asparagines, but it could grow fast and produce more conidiospore on the culture medium which carbon source comes from maltose, glucose and lactose, and nitro0gen source come from glycine, histidiane and peptone.

该菌在PH 4~12范围内均能生长、产生分生孢子并萌发,菌丝生长的最适pH 8~10。,孢子萌发的最适PH 7~9;该菌不能在以尿素和天门冬酰氨为氮源的培养基上生长,但以麦芽糖、葡萄糖、乳糖、淀粉、甘氨酸、组氨酸和蛋白陈为碳源和氮源时,菌丝生长快、产孢量大;光照处理对该菌菌丝生长、产孢和孢子萌发无显著影响。

When other factors are finable, 15L/h is the suitable amount of aeration.By analysis, the excessive aeration amount will destroy the capacity of sludge, and so decrease the removal efficiency of CODCr,and the 15L/h aeration amount is enouge for the demand of nitrification.

4蛭石应用于一级生物强化处理中,蛭石填料对有机物去除没有明显效果,但可以增加系统对氨氮去除能力,与对照组相比,高出30%多;吸附饱和后的蛭石,在活性污泥再生过程中,通过活性污泥的降解有机物,可去除较大部分的氨氮。

The main results are shown as follows:1.Screening freezing tolerant cultivars and lines of chrysanthemumTwenty seven lines of chrysanthemum grown outdoors were screened using \'injury rate\' and \'injury index\'.There were 7 cultivars and lines,which were relatively high tolerant to freezing treatment,i.e.,\'Jinbuhuan\',\'Xuetao\',\'Fuli\',\'Donglin 4\',\'Donglin 6\',\'Donglin 9\', and \'Donglin 13\'.There were 3 cultivars and lines,which were less tolerant,i.e.,\'Fenmeigui\', \'Donglin 1\',and \'Donglin 2\';The determination of electrolyte leakage,and contents of free proline,MDA,CAT,fructose,glucose and soluble sugars on 7 lines of chrysanthemums at different stages freezing showed that \'Xuetao\' and \'Donglin 4\' were more tolerant than others, and that especially \'Donglin 4\' was able to regulate its osmotic pathway to alleviate the \'injury degree\' caused by freezing conditions.2.Protein expression profile of chrysanthemum under low temperature stressTwo-dimensional liquid chromatography was applied to fractionate and analyze total protein taken from \'Donglin 4\' leaves grown at low temperature and at normal condition. Chromatography peaks of fraction were transformed to gel graph by ProteoVue software.

主要研究结果如下:1、露地菊抗冻品种和品系的筛选利用受伤率和伤害指数两项指标对27个露地菊品系进行耐低温筛选,筛选出'金不换\'、'雪涛\'、'富丽\'、'东林4号\'、'东林6号\'、'东林9号\'、'东林13号\'等7个耐低温品系,同时发现'粉玫瑰\'、'东林1号\'、'东林2号\'三个品系的耐低温能力最差;通过对不同自然低温处理时期,7个品系的电导率、丙二醛含量、脯氨酸含量、过氧化氢酶、果糖、葡萄糖和可溶性糖等指标的测定发现,'雪涛\'和'东林4号\'的耐低温能力要强于其它品系,尤其是'东林4号\'对低温的调节能力较强,在低温胁迫下,能够调节渗透途径减轻低温的伤害。2、低温胁迫下,露地菊蛋白质表达谱分析利用二维液相色谱技术,分离了'东林4号\'露地菊品系的低温处理和对照样品的总蛋白,通过ProteoVue软件分析发现:处理样品与对照相比共有蛋白差异点36个,其中显著增强表达的蛋白差异点有26个。

Amino acid analysis indicates that the contents of Ser,Thr,and Cys of the alkali treated rice protein decrease obviously under pH 13.5 at a glutelin concentration of 9%.

氨基酸分析表明,9%的谷蛋白含量、pH值为13.5碱处理大米谷蛋白的丝氨酸、苏氨酸、胱氨酸含量明显下降。

Taking piggery wastewater with high ammonia nitrogen and organic concentrations as treatment object,the ANAMMOX-based nitrosation process was investigated under the condition of ambient temperature of 25 ℃ and constant pH.

以高氨氮、高有机物浓度的养猪场废水为处理对象,在室温(25℃)、不调节pH的条件下,研究了基于厌氧氨氧化的亚硝化过程。

There was a little difference betweenphotosynthesis characteristic of the two varieties,response of Jiamusi to low temperature andpoor light was more sensitive than that of Longjiao No.2 and decreased pronouncedly withdecreasing light.6.EC and MDA of pepper leaves increased under low temperature and poor light,therewere no significant differences between them with decreasing light at 21-23℃/8-10℃,butthey were increased dramatically at 15-20℃/5-6℃.So we can speculate that lowertemperature was the direct reason resulted in cell membrane lipid peroxidation.7.No significant difference of SOD activity was observed under suboptimal temperatureand poor light21-23℃/8-10℃;150μmol·m~(-2·s~(-1),but SOD and POD activity substantiallydeclined with lowering temperature and light as well as treatment time.8.Under suboptimal temperature and lower light,proline accumulated to increaseosmotic regulation capacity and alleviate chilling damage.Soluble carbohydrate of pepper leaves decreased progressively and significantly with decreasing temperature and lightintensity as well as longer treatment time.So we think that synthesis and transport ofphotosynthetic product were inhibited by low temperature and poor light as solublecarbohydrate was important component of photosynthetic product.

两个供试辣椒品种之间的光合特性略有差异,佳木斯对低温弱光的反应比陇椒2号敏感,尤其随光照降低,其光合作用显著降低。6、低温弱光胁迫下,辣椒叶片的相对电导率和丙二醛含量都有不同程度的提高,在偏低温下,随着光照减弱,相对电导率和丙二醛含量差异不显著;在极端低温下,随着光照进一步减弱,相对电导率和丙二醛含量明显提高,随着处理时间延长,同一温度下,随着光照降低,叶片丙二醛含量也显著增加,由此可初步判断,低温是导致细胞膜脂过氧化的直接原因。7、经偏低温较弱光21~23℃/8~10℃、150μmol·m~(-2·s~(-1)处理后,辣椒叶片的超氧化物歧化酶活性变化与对照无显著差异,但随着温度、光强的逐步降低和处理时间的延长,SOD、POD活性显著降低。8、在偏低温较弱光下,辣椒能积累更多的脯氨酸,增强渗透调节,减轻低温冷害。

Forβ-l,3-glucanase,its activity increased to peak level after 2 to 5 days and was 2.02 and 1.34 folds of check,then decreased rapidly and resumed to normal level after 5 and 9 days.Generally Psoralea corylifolia L.extract may enhance the activities of chitinase andβ-l,3-glucanase in cucumber leaves,then induce the enhancement of activities of defensive enzymes.

从所测定的几种酶活性变化时间来看,补骨脂提取物处理黄瓜幼苗后,首先,使黄瓜体内的几丁质酶和β-1,3-葡聚糖酶等病程相关蛋白活性升高,然后在两者的作用下,诱导了黄瓜体内过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶等一些防御酶活性的提高。

This paper summarized literature about the structure, adsorption dynamics and thermodynamics, modification of zeolite and its application in wastewater treatment. Based on them, the conditions of zeolite modifying by AlCl_3 was explored, the adsorption properties natural zeolite to ammonia nitrogen and adsorption effects of aluminum modified zeolite to phosphor in solution was researched, the existing form of ammonia nitrogen was investigated by cyclic voltammeter.

本文在对沸石的结构、沸石吸附动力学、热力学、沸石改性及其在废水处理中的应用等相关文献综述的基础上,探索了用AlCl_3溶液改性天然沸石的条件,考察了天然沸石和铝改性沸石对溶液中氨氮的吸附性能以及对溶液中总磷的吸附效果,以及用循环伏安法探讨了水体中氨氮的存在形式。

The constructed wetland process was applied to treat rural domestic sewage. The results showed that the abilities of different macrophyte species to remove nitrogen and phosphorous were quite different with each other. Acorus calamus Linn has stronger ability to remove TP and NH3-N.

采用潜流式人工湿地工艺处理农村生活污水,结果表明:不同植物对氨氮、总磷的去除能力差别较大,菖蒲对总磷和氨氮净化效果较好。

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