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氨基嘌呤

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0Mg/L 6-benzylaminopurine was the optimal concentration of shoots quantity inducing which frequency of inducing shoots and number of shoots per explant were 100% and 11.1 respectively. 0.5mg/L 6-benzylaminopurine was the optimal concentration of shoots length inducing which average length of shoots was 12.0mm. Explant size had significantly effects on direct shoots quantity and length induction. When complete cotyledon used as explant, frequency of inducing shoots, number of shoots per explant and average length of shoots could approach to 100%, 6.7 and 17.1mm respectively. 2.0mg/L silver nitrate could significantly increase shoot quantity induction, both frequency of inducing shoots and number of shoots per explant could be as high as 97.2% and 4.2 respectively. But silver nitrate also could restrain shoots elongation.

结果表明:不同黄瓜品种直接不定芽数量诱导存在显著差异,但长度诱导无显著差异;6-苄氨基嘌呤对直接不定芽数量和长度的诱导作用显著,其中4.0mg/L为数量诱导适宜浓度,其出芽率和每外植体出芽数达到最高,分别为100.0%和11.1,0.5mg/L为长度诱导适宜浓度,其平均芽长12.0mm;外植体的大小对直接不定芽数量和长度均具显著影响,随着子叶的增大诱导直接不定芽的数量和长度增加,其中单片完整子叶为最适宜大小,其出芽率、每外植体出芽数和平均芽长分别达到100%、6.7和17.1mm;硝酸银也对直接不定芽数量诱导影响显著,其中2.0mg/L为适宜浓度,出芽率和每外植体出芽数分别达到97.2%和4.2,但对芽长有抑制作用。

The three day old seedlings grown in medium supplemented with 5 mg/L 6-BA were used to prepare the cotyledonary-node explants. The cotyledonary-node explants had one cotyledon and half of the embryonic axis.

选用在高达5 mg/L 6-苄氨基嘌呤(6-BA)的种子萌发培养基上生长3 d的蒺藜苜蓿幼苗,制备获得由1片子叶和半个胚轴组成的子叶节外植体。

The leaves, leafstalk, and stems from grapes were used as explants to induce calli and subculture in medium (100 mL B5 medium containing 3 g sucrose and 0.6 g agar) with different plant hormone combinations (6-BA, KT, IBA, 2, 4-D), and then the resveratol content from those calli was determinated by using HPLC.

以葡萄的叶、叶柄和茎分别作为外植体,在不同激素组合(6-卞氨基嘌呤(6-BA)、激动素、吲哚丁酸、2,4-二氯苯氧乙酸(2,4-D))的培养基(以B5培养基为基本培养基,含30g/L蔗糖和6g/L琼脂)上,进行愈伤组织的诱导和继代培养,并利用高效液相色谱法对不同来源愈伤组织中的白藜芦醇含量进行测定。

The variance of ABA of grape during storage were discussed in this paper.The following results are been drawn:the change of the content of ABA of grapes during storage characterizes by a parabolic shaped curve with obvious peak.Low temperature (0±0.5℃) is conducive to delaying the appearance of the peak of ABA and reduces the peak value.Nine kinds of plant growth regulators and two kinds of chemicals were chosen to treat grape bunches so as to study their mechanisms on ABA of grapes,2,3,5 triiodobenzoic a...

试验研究了葡萄贮期脱落酸的变化,结果表明:葡萄贮期 ABA含量呈抛物线形变化,有明显的高峰出现,低温( 0± 0 。5℃)贮藏具有推迟 ABA峰期和降低峰值的作用,经用 9种植物生长调节剂和 2种化学药品对葡萄果穗处理试验,三碘苯甲酸对 ABA的形成有极强的抑制作用,吲哚- 3-乙酸、赤霉素( GA3)、萘乙酸和 6-苄基氨基嘌呤( 6- BA)对 ABA的形成也具有拮抗作用,矮壮素、比久( B9)、乙烯利和外源脱落酸对 ABA的形成有促进作用。

Tetraploid veliger were carried out with four chemicals on this scallop in 1998—2000. Tetraploid were induced by inhibition of the polar body and first mitosis with cytochalasin B, colchicine, 6-DMAP and caffeine combined with CB, respectively.

中文摘要:1998—2000年采用细胞松弛素B、秋水仙素、6-二甲基氨基嘌呤(6-DMAP)以及咖啡因等药物抑制虾夷扇贝第一极体(PB1)释放、PB1和第二极体(PB2)释放以及抑制第一次卵裂等方法,诱导虾夷扇贝四倍体。

The regulation effects of 6 benzylaminopurine (BA, 0.05g/L) and ethyrel (ETH, 0.5g/L) on export of carbo assimilates from wheat leaf and its partition in the whole plant were studied using 14 CO 2 labeling method.

在盆栽条件下,采用活体测量和定期取样测定的方法,研究了6苄氨基嘌呤和乙烯利处理对小麦幼苗第1茎生叶14C同化物输出动态和向各器官中分配的影响。

In pot experiments, 6-benzylaminopurine and abscisic acid were brushed onto the ears, upper or lower blades of Echinochloa frumentacea to analyze the regulation of partial changes caused in the balance of endogenous hormone on Na(superscript +), K(superscript +) and free proline at whole plant level.

以盆栽的C4植物-湖南稷子为材料,用6-苄氨基嘌呤和脱落酸定位涂抹湖南枝子的穗、上位和下位叶片,分析了植物体激素平衡的局部改变对整株水平上Na+、K+和游离脯氨酸分配的调节。

Fertilizated eggs of abalone Haliotis discus Hannai were treated with cytochalasin B, cafeine and 6-dimethylaminoparine (6-DMAP) to block polar body Ⅰ, and the separated chromosomes of control and experimental groups were analysed.

分别用细胞松驰素B、咖啡因、6-二甲基氨基嘌呤处理皱纹盘鲍受精卵抑制第一极体后,分析了对照组和处理组受精卵染色体的分离情况。

Benzylaminopurine and abscisic acid were brushed onto the ears and upper leaves of Hordeum vulgore in pot experiments, to analyze the regulation of partial changes of hormone balance on distribution of Na(superscript +), K(superscript +) and free proline at a whole plant level.

用6-苄氨基嘌呤和脱落酸配合400mmol/L NaCl定位涂抹盆栽C3植物大麦的穗和上位叶片,分析了植物体激素水平的局部改变对叶片盐胁迫下整株水平上Na、K和游离脯氨酸分配的调节作用。

When the protoplast-derived calli were transferred into the MS solid medium added with 2.0mg/L benzylamino-purine and 0.4mg/L naphthaleneacetic acid and cultivated in the codition of low illumination, after which the adventitious shoots were induced, then we could obtain numerous plantlets and could transplant them survival to pots in the greenhouse.

当将原生质体分裂形成的愈伤组织转移到附加2.0mg/L6-苄氨基嘌呤和0.4mg/L萘乙酸的MS固体培养基上,并在低光照条件下培养后,从愈伤组织上分化出了不定芽,进而发展成小植株,并移栽成活。

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