氨基半乳糖

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Fucose composed of 40% the total carbohydrate with lesser but approximately equal amount of galactose, glucosamine, and galactosamine present.

对糖萼的糖化学组份分析表明糖萼是由40％的岩藻及大致等量的半乳糖，氨基葡萄糖和氨基半乳糖组成。

Urinary microalbumin was determined with immunoturbidimetry, urinary NacetylD-glucosaminidase,βDgalactosidase（GAL and glycyl proline dipeptidyl aminopeptidase were determined with rate, urinary creatinine was determined with Jaffe rate.

对127例糖尿病患者，采用免疫透射比浊法检测尿mA，速率法检测N乙酰βD氨基葡萄糖苷酶、βD半乳糖苷酶、甘氨酰脯氨酸二肽氨基肽酶，Jaffe速率法测尿肌酐。

The results show that all the fucoidan fractions had scavenging effects on superoxide, hydroxyl radical and hypochlorous acid, except that the high sulfated fractions F-B1 and F-B2 with low molecular weight had little scavenging activities on the three oxygen free radicals.

因低硫组分F一AZ(分子量8.7KD)在体外具有很好的抗氧化活性，因此选用四氯化碳(ee一4)和脂多糖+D一氨基半乳糖造成的急性肝损伤小鼠为模型，研究了F一AZ组分在这两种肝损伤小鼠体内的抗氧化活性和肝损伤的保护之间的关系。

The molecular compositions of PCE-CGH and PCE-CGL were investigated by chromatography and chemical analysis. Rhamnose, arabinose, glucose and galactose were proved to be the main strctural unit of PCE-CGH, and trifle glucoronic acid was identified in the molecule. The compositions of PCE-CGL were relatively complicated. Inositol and amino sugars besides for glucose and galactose were also identified in its molecule.

其中，PCE-CGH主要是以鼠李糖、阿拉伯糖、葡萄糖和半乳糖为结构单元的大分子多糖类物质，并含有少量的己糖醛酸；PCE-CGL的分子组成相对复杂，除含有葡萄糖和半乳糖外，还含有一定量的肌醇和氨基糖。

Methods We developed hepatic failure by two methods.(1) Surgical moldel of hepatic failure: Under general anesthesia, an end-to-side portocaval shunt was performed in seven trial minipigs. Forty-eight hours later after operation, these minipigs underwent transient total liver ischemia for 4 hours by ligating the hepatoduodenal ligament.

应用中国实验小型猪7头，采用Ⅰ期门静脉－下腔静脉吻合、Ⅱ期（48h后）供肝动脉暂时结扎4h的方法建立缺血性急性肝功能衰竭动物模型；中国实验小型猪6头给于1.2g/kg的D-氨基半乳糖诱导肝功能衰竭，建立药物性急性肝功能衰竭动物模型。

Results:It is suggested that the drug significantly protectes liver from CCl4,thioacetamide and galactosamine in mice and rats,lowers the activity of enzyme ALT in serum,shorten the sleep duration of CCl4-damaged mice induced by embuter,inhibites HBsAg in vitro,and potentiates non-specific immunity in mice and rats.

结果：研究表明，该药对四氯化碳、硫代乙酰胺和氨基半乳糖引起小鼠及大鼠的急性肝损伤有明显的保护作用，能降低谷丙转氨酶；缩短CCl4肝损伤小鼠戊巴比妥钠的睡眠时间；抑制体外乙肝病毒表面抗原；对非特异性免疫有一定增强作用。

Study on Gene Cloning, Expression and Expression Profiling in Tissues of Polypeptide: N-acetylgalactosaminyl Transferase 2

多肽：N-乙酰氨基半乳糖转移酶2（pp-GalNAc-T2，E.C.2.4.1.4）的基因克隆、表达及组织表达谱研究

Experimental Center of National Level One, Hubei Traditional Chinese Medica l College, Wuhan 430061）Abstract To investigate the protective effects of Jiaweisinisa n on acute liver injury induced by intraperitoneal injection of D-ga lac-tosamise, serum superoxide dismutase, malondialdehyde , glutathioe contents were measured. The pathological changes of liver wer e observed simultaneously. The results showed that JWSNS could decrease serum MD A content, but increase serum SOD and GSH activities in acute liver injury induc ed by D-GalN. It was also found that JWSNS could alleviate the degeneration and necrosis of the hypatocytes.

为研讨加味四逆散防治肝损伤的作用机理，通过动物试验，先用加味四逆散预防给药，再采用D-氨基半乳糖造成大鼠急性肝损伤模型，然后检测大鼠血清超氧化物岐化酶、丙二醛、还原型谷胱甘肽水平，同步观察该方对急性肝损伤大鼠体内脂质过氧化反应及肝脏病理改变的影响，结果表明该方可明显升高大鼠血清SOD、GSH水平，降低MDA水平，肝脏病理学检查亦表明该方使肝细胞变性坏死程度减轻。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。