氨基化

Methods The viral particles of recombinant adeno-associated viral vector 2-IRES-EGFP/MnSOD(rAAV2-IRES-EGFP/MnSOD) were injected into the perilymph through the round window membrane on the 6th week.To observe the feasibility of MnSOD gene therapy for protecting the cochlear function with the methods of Westernblot, ApopTag peroxidase apoptosis detection, MnSOD activity detection and effect on hearing.

方法在半乳糖注射的第6周(注射氨基糖甙类抗生素之前2周)将rAAV2-IRES- EGFP/MnSOD病毒液经圆窗膜注入拟老化大鼠的耳蜗外淋巴，通过免疫组化(ApopTag过氧化物酶凋亡检测)、Westernblot及MnSOD活性检测的方法观察MnSOD基因在对抗内耳氧化应激损伤中的干预性保护作用。

In a system consisting of 20% PEG 400 and 12% magnesium sulfate, the partition coefficient of PGA was less than 0.01, PGA almost completely partitioned into the magnesium sulfate-rich bottom phase. Meanwhile, the partition coefficient of cephalexin was 6.7, the partition coefficients of phenylglycine methyl ester and 7-ADCA were 1.5 and 1.2, respectively.

通过考察头孢氨苄、苯甘氨酸甲酯和7-氨基脱乙酰氧基头孢烷酸(7-ADCA)在ATPS中的分配行为建立了一个含20%PEG 400和12%硫酸镁的ATPS，在此体系中头孢氨苄的分配系数为6.7，苯甘氨酸甲酯的分配系数为1.5，7-ADCA的分配系数为1.2，且青霉素酰化酶的分配系数小於0.01。

Enzymatic synthesis of cefaclor from 7\|aminodesacetoxymethyl\|3\|chlorocephalosporanic acid (7\|ACCA) and phenylglycine derivatives using penicillin G acylase was studied.

以7-氨基去乙酰氧甲基-3-氯头孢烷酸(7-ACCA)和苯甘氨酸衍生物为底物，利用青霉素酰化酶酶促合成头孢克罗。

This new method used in this paper for porcine kidney aminoacylase purification has both theoretical and practical significance.

本文所建立的分离纯化和冷冻干燥氨基酰化酶的方法都具有一定的使用价值。

L-Amino-2-bromo-4-hydroxy- anthraquinone could be obtained by coordinated hydroxylation of l-amino-2- bromo- anthraquinone, boric acid and the initiator potassium iodide or bromide with 97.5%yield and 99% purity.

在用HPLC跟踪溴氨酸的合成过程研究中发现，1-氨基蒽醌-2，4二磺酸在溴化过程中可转化为产物溴氨酸。

The influence of the position of amino associated with pyrimidine on the reaction yields was discussed.

以取代的氨基嘧啶为共试剂，与多种芳香族硝基化合物进行了硒催化的羰基化反应，产率最高可达90％左右。

Objective To investigate the roles of antioxidant, pyrrolidine dithiocarbamate in severe acute pancreatitis.

目的 探讨抗氧化剂四氢化吡咯二硫代氨基甲酸脂对重症急性胰腺炎的抑制作用。

Methods: 72 Wistar rats were randomly divided into sham-operation group, SAP group, and SAP previously treated with pyrrolidine dithiocarbamate group.

Wistar大鼠72只，随机分为三组：假手术组；重症急性胰腺炎组；四氢化吡咯二硫代氨基甲酸脂治疗组。

METHODS:After the treatment of TRAIL or LPS at different doses,we tested the nuclear translocation of NF-κB by cell immunohistochemical staining and electrophoretic mobility shift assay,and evaluated the level of IκB by RT-PCR under pyrrolidine dithiocarbamatetreatment.

当不同浓度的TRAIL和LPS作用于细胞后，我们通过细胞免疫组化染色和凝胶电泳迁移试验来检测NF-κB核转位的情况。并通过RT-PCR的方法粗略评定二硫代氨基甲酸吡咯烷对抑制蛋白IκB的影响。

The five quinazoline compounds were prepared by using 2 amino 4,5 dimethoxy benzoic acid as original material, which underwent ring closing, halogenation and substitution.

以2 氨基 4，5 二甲氧基苯甲酸为原料，分别经过环化、氯代、取代等反应，合成了五种目标化合物。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。