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氧合血红蛋白

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According to absorption, the fractional saturation versus the partial pressure of oxygen can be obtained by means of absorption kinetics.

根据吸附过程的特点,利用吸附动力学方法,得到了反映血红蛋白分子氧合过程的氧饱合分数与氧分压之间的关系式。

It demonstrates that a sigmoidal curve of oxygenation represents the real allosteric effect that apperas in a Hb molecule during oxygenation.

这表明S形的氧合曲线实际显示了单个血红蛋白分子的别构效应。

Previous studies by others and this laboratory on the allosteric effect of hem oglobins have suggested that the electrostatic replusive cavity at the center of tetrameric moiety plays a substantial role in regulating O2 transport prope rties, including chloride binding, and Bohr effect .

中文摘要过去对血红蛋白异位调节效应的研究指出,在血红蛋白四合体中央空腔的区域的静电排斥效应对分子机能的调节如氧合时氯离子的键结和波尔效应,扮演著一个很重要的角色。

In attempt to obtain a further insight into the contribution of charged residues to other allosteri c properties , in this study we designed and synthesized a site directed mutan t , b82 Lys-Asn , for examing influencs the tetrameric stability and enthalpit ic change in O2 binding of human adult hemoglobin.

本研究焦点放在血红蛋白中央空腔中,带有正电残基的其中一个位置,b82 Lysyl ,来研究其正电离子效应与人类血红蛋白氧合反应热及四合体安定性的相关。针对这个目标,我们利用电脑做胺基酸序列排列比对和分子结构的模拟,选择以Asparaginyl残基取代Lysyl残基。

By O2 equilibrium, gel permission chromatography, and autooxidation analyses, we have investigated that the pos itively charge of b82 Lysyl residue is indeed involved in the exhothermic O2 b inding characteristics and somewhat stabilize the tetrameric heme moiety of Hb A.

其纯度以可见光谱和血色素电泳鉴定,并以氧合平衡曲线的测定,胶质层析和氧化速率的分析检视重组血红蛋白之性能。实验结果显示β82Lysyl残基所带的正电性确实影响血红蛋白氧合时的放热反应与其四合体的安定性。

As oxygen pressure increases, the conformation of hemoglobin will undergo a series of intermediates from deoxygenated form to oxygenated form.

当氧分压的值从小到大变化时,血红蛋白分子将从脱氧构象态经历一系列中间构象态到达完全氧合构象态。

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