氧化测定法

While erthrocyte, hemoglobin and Hela cell were under oxidative damage induced by the hydrogen peroxide (H2O2) or tert-butyl hydroperoxide on different concentrations of dihydromyricetin, hemolysis rate of erythrocyte and methemoglobin converted from hemoglobin was estimated by ultraviolet-visible absorption spectroscopy. Whereas mmalondialdehyde from erythrocyte was measured by thiobarbituric acid colorimetry, the cellular viability of Hela cell was examined using MTT 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay.

在不同的二氢杨梅素浓度下，用过氧化氢或过氧化特丁烷对红细胞、血红蛋白和Hela细胞进行氧化伤害，以紫外－可见光分光光度法检测红细胞溶血率和由正常血红蛋白生成的高铁血红蛋白，硫代巴比妥酸法测定红细胞中的丙二醛，MTT法检测Hela细胞的活力。

Additionally, the oxidation degree or thealdehyde contents were measured by using photometry and titration methods.

采用直接法和间接法测定了氧化海藻酸钠的氧化度，以粘度法表征了相对分子质量。

The antioxidant activity of Smilax glabra was determined by DPPH·method, the results showed thatSmilax glabra which were collected from different areas has antioxidant activity, but the antioxidantactivity of Smilax glabra didnt has fixed relationship with the content, so we suggested that the mechanismof action was cooperation between different components.

采用DPPH·法测定不同产地土茯苓总提取物的抗氧化活性，实验结果表明广东韶关土茯苓总提取物抗氧化活性最强，但总提取物的抗氧化活性与白藜芦醇的含量没有呈正相关系，因此我们提出土茯苓的抗氧化活性可能是各成分之间的共同作用。

In order to determine the saturated vapor pressure of colloid ed dinitrogen tetroxide,and find out its change rule with the various temperature,a testing equipment of determining satuated vapor pressure of colloid ed dinitrogen tetroxide was designed and constructed according to the principle of static testing.

为了测定胶体四氧化二氮的饱和蒸气压，找出其在不同温度条件下的变化规律，采用静态法测试原理，自行设计建立了四氧化二氮胶体饱和蒸气压的测试装置，测得了常压下液体和胶体四氧化二氮的饱和蒸气压数据，结果表明，四氧化二氮体系成胶后比成胶前的饱和蒸气压略有降低，且温度越高其差值越大。

Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride（ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

用热水提取，乙醇沉淀提取粗多糖，再经sevag法去除蛋白，透析去除小分子物质，冷冻干燥得楮头红多糖粗品；采用deae-52纤维素柱层析和sephadex g-200凝胶柱层析进行分离纯化；用tba法考察多糖的抗脂质过氧化作用；采用ccl4急性肝损伤模型，测定其血清中ast变化。结论获得一个楮头红多糖组分。tba法表明该组分对小鼠自发性脂质过氧化具有显著的抑制作用；在保肝试验中，给药组能明显抑制ast的升高，对肝损伤有一定的保护作用。

Chinensis and Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible NOS activity also decreased significantly with the time elapsed in WSSV infected shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization test showed that WSSV of the two penaeid species couldn't be detected until iNOS started decreasing.

在此基础上，我们建立了对虾血细胞中诱导型一氧化氮合成酶的鉴定方法—硝基四氮唑蓝还原法和血细胞形态法以及酶活力的测定方法—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件，结果显示：对虾血细胞在4℃下与L-精氨酸和LPS孵育8h以及当L-精氨酸浓度为2.5mmol l〓LPS浓度为100μg ml〓，Ca〓浓度为2.5mmol l〓时，iNOS活力的测定结果最佳。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

The superoxide dismutase activity, lysozyme activity and the total haemocyte counts of the juvenile shrimp were detected with xanthine-oxidation method, advanced Hultmark method, and haemocytometer method, respectively.

以黄嘌呤氧化法测定对虾腹肌中的超氧化物歧化酶活性，采用Hultmark等人的改进方法测定溶菌酶活性，以血球计数板法测定总血淋巴细胞密度。

The content of lipid peroxide was determined with fluorescence method, and the activity of superoxide dismutaseand glutathione peroxidase was determined with self-oxidation method of pyrognllol and Hafenam method, respectively.

脂质过氧化物含量按荧光法测定，超氧化物歧化酶活性应用邻苯三酚自氧化法测定，谷胱甘肽过氧化物酶活性按Hafenam方法测定。

METHODS Dinitrofluorobenzene was used to induce the contact dermatitis in mouse ear, rhIL-11 0.375-1.5 mgkg^(-1)d^(-1) sc for 10 d, the degree of skin inflammatory reaction was observed. At the same time, the serum level of tumor necrosis factor-α and interleukin-6(IL-6) was measured by radioimmunoassay; the serum level of nitrogen monoxidum was detected by biochemical method and the expression of intercellular adhesion molecule-1(ICAM-1) was detected by immunohistochemical method.

采用2，4-二硝基氟苯致小鼠耳部皮肤接触性皮炎试验模型，观察sc rhIL-11 0.375~1.5mgkg^(-1)d^(-1)，连续10d后皮肤炎症反应程度，同时采用放射免疫分析法测定血清中肿瘤坏死因子-α和白细胞介素-6(IL-6)的水平，采用生物化学检测法测定血清一氧化氮的水平，采用免疫组化法测定皮肤中细胞间粘附分子(ICAM-1)的表达。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。