氧化测定法

The methods determing the groups in the modified starch have been developed, that is acidimetry for the basicity of acidified starch, complexing titration for the substitution degree of carboxymethyl starch, and heat paste titration and carbonyl process respectively for the carboxyl and nitrogen of oxidized starch.

建立了以酸碱滴定测定酸化淀粉的碱量、络合滴定法测定羧甲基淀粉的取代度、热糊滴定和羟胺法测定氧化淀粉中羧基及羰基、消化定氮测定阳离子淀粉等几种变性淀粉的基团分析方法。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只，随机分为对照组，缺血再灌注组和缺血预适应组，每组6只，分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平；免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

Methods: The sample extraction method of total flavonoids, including water bath water bath reflux extraction and ultrasonic extraction method, the sample determination of total flavonoids, including aluminum nitrate chromogenic method and direct color method, the sample determination of antioxidant flavonoids, including FRAP method and DPPH method.

样品总黄酮提取方法包括恒温水浴回流提取法和恒温水浴超声波提取法，样品总黄酮含量测定方法包括硝酸铝显色法和直接显色法，样品总黄酮抗氧化性测定方法包括FRAP法和DPPH法。

An ion-chromatography method was described for the determination of Cu, Ni and Zn in scandium oxide.

离子色谱法测定氧化钪中的铜、镍、锌潘炳贤，周春山，熊兴安，王艳，徐卫红（中南工业大学化学化工新技术研究所，长沙，410083）摘要介绍了离子色谱法对氧化钪中的Cu，Ni，Zn等元素的分离和测定。

The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.

结果： 通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。

METHODS: Cultured rat cardiomyocytes were induced to be hypertrophy by angiotensin Ⅱ and norepinephrine. Glucose oxidation rate, glucolysis rate and fatty acid oxidation rate were determined by liquid scintillation counting, and cell apoptosis was detected by TUNEL.

应用血管紧张素Ⅱ(AngⅡ， 0.1mol/L)+去甲肾上腺素(NE 1mol/L)诱导培养大鼠心肌细胞肥大，以同位素液闪计数法测定葡萄糖有氧氧化率、葡萄糖酵解率和脂肪酸有氧氧化率，TUNEL法测定细胞凋亡。

In the paper, the multidentate carboxylic acid ligands were used to combine with Cd, resulting in the three dimensional MOCPs single crystals—C12H16CdN2O8, with a low temperature hydrothermal synthesis method. The sample was characterized with IR spectra to affirm the organic groups in the frameworks, single-crystal X-ray diffraction to determine the crystal structure and composition of the frameworks, N2 adsorption isotherms at low temperature to measure the specific surface area and pore size distribution, and with TGA/DGA analysis to scale the thermal stability. Furthermore, the dissolution property and the photocatalytic activity of MOCPs under the ultraviolet ray condition were explored.

本文用低温水热合成法，用多齿羧酸配体异烟酸和Cd配位合成出[Cd(C6H4O2N)2(H2O)4]∞三维晶体并进行了表征：采用红外光谱法确认骨架中的有机官能团；用单晶X射线衍射分析法测定MOCPs的晶体结构，并确定其骨架的组成；通过低温氮气吸附等温线测定了其比表面积和孔径分布；以热重分析和差热分析测定材料骨架的热稳定性；测定了晶体的溶解性能；最后以亚甲基蓝为目标降解物，对[Cd(C6H4O2N)2(H2O)4]∞的光催化活性及亚甲基蓝的氧化动力学进行了探索(来源：A2444BC论文网www.abclunwen.com)。

Soil organic matter, total nitrogen, available nitrogen and pH are measured with high temperature potassium dichromate oxidation-capacity, semiminim karl method, alkaline hydrolysis pervasion method, and the potentionmeter method respectively.

用五点混合取样法采集广西桂林毛村岩溶区和非岩溶区典型林地、灌草丛地和耕地的0~20cm，20~40cm土壤样品，以高温外热重铬酸钾氧化－容量法测定有机质含量，半微量凯氏法测定全氮含量，碱解扩散法测定有效氮含量，电位法测定pH值。

Solubility of barium chloride and barium oxide in hydrochloric acid, ethanol,acetone,ether and hydrochloric acid- ethanol,hydrochloric acid- acetone,hydrochlo- ric acid- ether has beeen studied and the separation of Ba O from Ba Cl\- 2 with hydrochloric acidethanol as the solvent has been proposed.

研究了氯化钡和氧化钡在盐酸、乙醇、丙酮、乙醚及盐酸-乙醇、盐酸-丙酮、盐酸-乙醚中的溶解性，提出以盐酸-乙醇为溶剂进行两者的分离。以 DBC- CPA为显色剂分光光度法测定氧化钡的含量，加料回收率为 98%～ 10 2 %，氧化钡含量为 0 。32 %时，平行测定 7次，相对标准偏差为 1。 58%。该法适于快速测定热处理盐浴中氧化钡的含量，对监控盐浴品质有指导意义

Methods A routine culture system of melanocytes was established, cells were irradiated with different dosages of UVB(30 and 90mJ/cm2 respectively), and then incubated with different concentrations of TMP for 72h. MTT method, NaOH assay and DOPA oxidization reaction were employed to determine the cell proliferation, melanin synthesis, tyrosinase activity of melanocytes.

常规分离培养正常人黑素细胞，分别用30mJ/cm2、90mJ/cm2的UVB照射细胞，然后加入100μg/ml、300μg/ml、600μg/ml的TMP孵育至72h，采用噻唑蓝法测定细胞增殖情况；多巴氧化法测定酪氨酸酶活性；NaOH裂解法测定黑素含量。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。