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METHODS: Fortyfive aged healthy crossbred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively.

将45只犬分为川芎嗪,维拉帕米和对照组3组,结扎LAD阻断血流75%,分别测定3组结扎前及结扎后不同时段的分泌型P选择素、内皮素1(ET1)、一氧化氮及心肌肌钙蛋白T的含量变化,Western blotting法检测心肌组织Pselecntin基因表达情况。

METHODS: Fortyfive aged healthy crossbred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET1, sPselectin and CTnT before ligation and at different time points after ligation were detected and Pselectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪,维拉帕米和对照组3组,结扎LAD阻断血流75%,分别测定3组结扎前及结扎后不同时段的分泌型P选择素、内皮素1(ET1)、一氧化氮及心肌肌钙蛋白T的含量变化,Western blotting法检测心肌组织Pselecntin基因表达情况。

METHODS: Fortyfive aged healthy crossbred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET1, sPselectin and CTnT before ligation and at different time points after ligation were detected and Pselectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪,维拉帕米和对照组3组,结扎LAD阻断血流75%,分别测定3组结扎前及结扎后不同时段的分泌型P选择素、内皮素1(ET1)、一氧化氮及心肌肌钙蛋白T的含量变化,Western blotting法检测心肌组织Pselecntin基因表达情况。

METHODS: Forty five aged healthy cross bred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET 1, sP selectin and CTnT before ligation and at different time points after ligation were detected and P selectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪,维拉帕米和对照组3组,结扎LAD阻断血流75%,分别测定3组结扎前及结扎后不同时段的分泌型P 选择素、内皮素 1(ET 1)、一氧化氮及心肌肌钙蛋白T的含量变化,Western blotting法检测心肌组织P selecntin基因表达情况。

In order to explore the role of cytochrome c oxidase during the development of Microtus brandti, the activity of mitochondrial CcO and content of mitochondrial protein in voles' liver was measured by using ultraviolet spectrophotometry, these voles were chosen according to days.

为探讨线粒体能量代谢中细胞色素c氧化酶(cytochrome c oxidase,CcO)在布氏田鼠生长发育过程中的作用,应用紫外分光光度法测定了不同日龄的幼鼠肝脏内线粒体蛋白含量及CcO活性。

objectiveto investigate the effect of myricetin dispersible tablets on experimental cerebral ischemia-reperfusion injury in rats and thrombosis formation.methodsexperimental cerebral ischemia-reperfusion injury model was induced by ligation bilateral common carotid arteries. the protective effect of myricetin dispersible tablets was observed by detecting the content of cerebral water, sod and mao activities,mda and la contents.

方法采用双侧颈总动脉结扎法制备大鼠实验性全脑缺血再灌注损伤模型,通过测定大鼠脑含水量,超氧化物歧化酶、单胺氧化酶的活性和丙二醛、乳酸的含量,观察杨梅素分散片对大鼠实验性脑缺血的影响;采用体内静脉血栓形成法,观察杨梅素分散片对体内静脉血栓形成的影响;并研究其对血小板聚集的影响。

MethodsExperimental cerebral ischemia-reperfusion injury model was induced by ligation bilateral common carotid arteries. The protective effect of myricetin dispersible tablets was observed by detecting the content of cerebral water, SOD and MAO activities,MDA and LA contents.

方法采用双侧颈总动脉结扎法制备大鼠实验性全脑缺血再灌注损伤模型,通过测定大鼠脑含水量,超氧化物歧化酶、单胺氧化酶的活性和丙二醛、乳酸的含量,观察杨梅素分散片对大鼠实验性脑缺血的影响;采用体内静脉血栓形成法,观察杨梅素分散片对体内静脉血栓形成的影响;并研究其对血小板聚集的影响。

A new catalytic spectrophotometry was established for the determination of trace silver based on the catalytic effect of Ag on the oxidational discoloration reaction of methyl violet with potassium persulfate in buffer medium of HAc-NaAc including phenothroline as activator.

在醋酸-醋酸钠缓冲介质中,有活化剂邻二氮菲存在时,Ag对过硫酸钾氧化甲基紫褪色反应有催化作用,建立了测定痕量银的新催化光度法。

A persulphate oxidation method in acid medium for decomposition and analysis of dissolved total phosphorus in seawater was proposed.

依此,建立酸性过硫酸盐氧化法分解测定海水中溶解态总磷的方法。

A catalytic kinetic spectrophotometric method has been established for the determination of trace Mn, based on the catalytic action of Mnon the oxidation - reduction reaction between KIO4 and KI in the presence of 1, 10 -phenanthroline and in HAc-NaAc buffer solution.

本文研究了HAc-NaAc缓冲溶液中,在1,10—菲啰啉激活作用下,锰对KIO4-KI氧化还原反应的催化动力学条件,建立了催化动力学光度法测定痕量锰的方法。

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