氧化测定法

Antioxidative activity of three naringin-metal complexes was characterized by applying methods of 1,1-diphenyl-2-picrylhydrazyl radical and thiobaribituric acid.

采用二苯代苦味肼基自由基法和硫代巴比妥酸法对3种柚皮苷-金属配合物的抗氧化活性进行了测定。

The number of EOS in peripheral blood were counted by counting plate and the morphological changes in bronchia and EOS infiltration in lung tissue were observed. The content of NO and ET-1 in blood serum and BALF were measured by biochemistry and radioimmunity methods.

用计数板计数外周血嗜酸性细胞数量，观察小支气管形态学改变和肺组织中EOS浸润情况；生化法和放免法测定血清和肺泡灌洗液中一氧化氮和内皮素－1(ET-1)的含量。

According to the condition of laboratory and the practices of more than 15 years,we improved the condition for calefaction and oxidation of capacity measuration GB1999,and we explored two better methods of soil organic matter determination: oven torrefaction heating method and digestor heating method.

根据实验室的条件和多年的实践，对容量法GB1999加热氧化控制条件进行改进，摸索出两种比较理想的土壤有机质测定方法：烘箱加热和消化炉加热法。

A method for the determination of silicon and phosphorus in antimony oxide by inductively coupled plasma emission spectrometry ICP-AES was reported. he sample dissolution methods, election of spectral lines, interference of coexistent elements and the working parameters of the instrument were studied.

通过对氧化锑试样分解方法、硅磷分析线选择、共存元素光谱干扰和仪器最佳分析条件等方面的研究，建立了电感耦合等离子体原子发射光谱法测定氧化锑中硅和磷的方法。

Six crossbred steers (Simmental×Indigenous yellow cattle) with cannulas in rumen,duodenum and terminal ileum are used for determining the effects of feeding level on synthesis efficiency of rumen microbial protein.

用6头装有瘤胃、十二指肠、回肠瘘管的西门塔尔杂交阉牛，以Cr2O3为指示剂测定食糜流量，用嘌呤氧化法测定RNA当量，研究不同饲养水平下，西门塔尔杂交阉牛瘤胃微生物蛋白的合成效率。

Eleven α-V-group cation-substituted toluenes (1) and acetophenones (2)were synthesized. The equilibrium acidities of these compounds were determined indimethyl sulfoxide solution by the "Overlapping Indicator Method" and the homolytic C-Hbond dissociation energies were derived from a thermodynamic cycle combining pKawith oxidation potential of carbanion.

合成了α-位有第V、Ⅵ主族元素杂正离子取代基的甲苯系列（1）和苯乙酮系列（2）共11个Ylide前体化合物，用重迭指示剂法首次测定了其在二甲亚矾溶剂中的酸性解离常数PKa，同时结合碳负离子氧化电位的测定，通过热力学循环估测了它们的气相均裂键能。

Comparision on antibacterial activity of ethanol extracts of clove bud and clove fruit in vitro;2. The results showed that ethanol extracts from C.

法测定了油茶叶乙醇提取物的抗氧化活性，并与芦丁、二丁基羟基甲苯进行了比较，在波长517nm、反应时间40min的条件下测定了DPPH。

Twelve L5 left dorsal root ganglions on each time point were harvested from sham group on the 7th day after operation and from model group on the 7th,14th and 21st day after operation and then were tested histologically by Hematoxylin-Eosin staining and the proportion of Cycloxygenase-2（COX-2） and p-p38MAPK positive expression cell were tested by immunohistochemistry method either.

测量各组大鼠术前至术后21d时左后肢50％机械性撤足阈值（50%PWT）以测定机械痛敏的变化，假手术组术后7d及模型组术后7d、14d、21d取左L5 DRG分别进行HE染色观察组织学变化和免疫组化法测定环氧化酶-2（COX-2）与p-p38MAPK的阳性细胞比率。

Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括：[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术；[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离，测定了不同顺式异构体的吸光系数，建立了天然番茄红素几何异构体组成的检测方法，为区分和鉴定人工合成品和天然产物奠定了基础；[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值，并建立了超临界CO_2萃取的实验室方法；[4]探索了提高番茄红素超临界流体萃取效率的方法，证明了物料前处理对提高萃取效率起了极其重要的作用；[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况，发现了大量顺式异构体的存在，并证实了几何异构体组成在血清中发生了显著变化；[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律；[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力；[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能；[9]首次发现大鼠前列腺内存在番茄红素C31降解片段，并证实其仍具有淬灭单线态氧的能力。

Methods Male SD rats were given drinking water containing manganese chloride (MnCl24H2O 0.5g/L and 5.0g/L respectively) for 60 days. At the end of experiment, all the rats of the manganese-exposed groups and control group were decapitated after anaesthesia with 2% sodium phenobarbitone intraperitoneally, the brains were removed and activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphate, cholinestarase, monoamine oxidase, superoxide dismutase, nitric oxide synthase, and levels of glutathione, maleic dialdehyde, and proteins were biochemically analyzed.

大鼠经饮水连续染锰（MnCl24H2O 0.5g/L和5.0g/L）60d后，应用荧光光度比色法测定新鲜脑组织匀浆的谷丙转氨酶、谷草转氨酶、碱性磷酸酶、胆碱酯酶、单胺氧化酶、氧化亚氮合酶、超氧化物歧化酶活性和谷胱甘肽、丙二醛、蛋白质含量，并设生理盐水对照组。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。