氧化测定法

In this thesis, we used this principle coupled with flow injection solid phase reactor on-line oxidation, solid phase extract and dialysis separation technology to establish the on-line oxidizing fluorometric method for determination thioridazine hydrochloride and methotrexate in table and plasma samples, respectively.

第二章：研究报告一、流动注射在线氧化荧光法盐酸硫利达嗪分析体系研究流动注射在线氧化荧光法测定药物中盐酸硫利达嗪含量本方法根据盐酸硫利达嗪的氧化产物具有强荧光这一性质，在磷酸介质中利用二氧化铅固相反应器在线氧化盐酸硫利达嗪并通过乙醇对其的增敏作用，建立了流动注射在线氧化荧光法测定盐酸硫利达嗪含量的新方法。

Serum level of TNF-α was measured by unsaturation assay; Serum level of IL-2、IL-6、 IL-10 were measured by radioimmunoassay.

采用非饱和法测定肿瘤坏死因子α的水平；采用放射免疫法测定白介素-2(IL-2)、白介素-6(IL-6)和白介素-10(IL-10)的水平；采用酶联免疫吸附法测定肌钙蛋白Ⅰ的水平；采用速率法测定丙氨酸转换酶的水平；采用肌氨酸氧化酶法测定肌酐的水平。

We detected fasting plasma glucose by glucose oxidase,fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology,tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index,that is,ISI=1/FPG×FIns.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

We detected fasting plasma glucose by glucose oxidase, fasting insulin by chemiluminescent immunoassay, triglyceride and high density lipoprotein-cholesterol by zymology, tumor necrosis factor-αby enzyme linked immunosorbent assay, the reverse of the product of FPG and FIns is used as the insulin sensitivity index, that is, ISI=1/FPG×Fins.

血糖测定采用葡萄糖氧化酶法；胰岛素测定采用化学发光法；血脂（甘油三酯TG、高密度脂蛋白HDL-C）测定采用酶法；肿瘤坏死因子-α测定采用双抗体夹心酶联免疫法；胰岛素敏感指数采用空腹血糖与空腹胰岛素乘积的倒数，即ISI=1/FPG ×FIns。

reitman method to measure ALT and AST; bilirubin oxidase method to measure total bilirubin and conjugated bilirubin; biuret method to measure serum general protein; bromocresol purple method to measure serum albumin; enzymic colorimetric method to measure serum bile acids; benzyl aldehide azo naphthol method to measure monoamine oxide.

赖氏法。测定 ALT 和 AST；胆红素氧化酶法测定总胆红素和结合胆红素；双缩脲法测血清总蛋白；溴甲酚紫法测定血清清蛋白；酶比色法测定血清胆汁酸；苄醛偶氮萘酚法测定单氨氧化酶

The precision, linearity, anti -interference ability, recovery rate were compared among the 4 methods according to the standard method of NCCLS, and relativity between each of the 4 methods and modified diazotate J - G was calculated. Results

结果改良化学氧化法、钒酸氧化法、胆红素氧化酶法的批内精密度均小于3.0％；4种方法的回收率均在95.0％～105.0％之间；KHC3法、改良化学氧化法、钒酸氧化法和胆红素氧化酶法的线性范围分别是：0～424μmol／L、0-675μmol／L、0-629μmol／L、0-575μmol／L；胆红素氧化酶法和改良化学氧化法均有较强的抗干扰能力，和改良J-G法的测定结果有高度一致性。

Reproducibility, recovery rate, filate range, interference of haematolysis and lipaemia were evaluated by NCCLC system, and then the results were compared.

用钒酸氧化法、改良J—G法和胆红素氧化酶法测定血清中的总胆红素，按NCCLC评价方案系统评价其重复性、回收率、线形范围及溶血、脂血对胆红素测定的干扰影响，然后对测定结果进行比较。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估：手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量；应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平；以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜；以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

The product has green-yellow fluorescence at excitation wavelength of 355 nm and emission wavelength of 478 nm, and the fluorescence intensity could be enhanced in the presence of acetone.

三、流动注射在线氧化荧光法测定氯普噻吨的的研究利用在硫酸介质中二氧化铅可使无天然荧光的氯普噻吨氧化而转变为在最大激发波长和最大发射波长分别为393nm和452nm的强荧光物质，而且丙酮对该荧光体系有强烈增敏作用的现象，建立了以醋酸纤维素固定化的二氧化铅作为固相反应器的流动注射在线氧化荧光法测定氯普噻吨的新方法。

Flow injection fluorometry for the determination of carbamazepine in pharmaceuticals and plasmaA simple, sensitive and specific spectrofluorimetric method has been developed for the determination of CBZ. The method was based on lead dioxide solid-phase reactor flow injection on-line oxidizing CBZ into fluorescent product in medium of phosphoric acid. The product has green-yellow fluorescence at excitation wavelength of 355 nm and emission wavelength of 478 nm, and the fluorescence intensity could be enhanced in the presence of acetone. The reaction conditions and experimental conditions were studied and optimized.

二、流动注射在线氧化荧光法测定卡马西平的研究利用卡马西平自身无荧光，经过氧化后可转变为在最大激发波长为355nm，最大发射波长为478nm的强荧光物质，而且丙酮对该荧光体系有增敏作用的现象，应用流动注射体系建立了以醋酸纤维素固定化的二氧化铅作为固相反应器、在磷酸介质中在线氧化荧光法测定卡马西平的新方法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。