氢化酶

Biogenetic gas;origin of hydrogen;biochemical action;hydrogenase;isotopic composition

生物气；氢的来源；生物化学作用；氢化酶；同位素组成

Chemical mimic of the Fe-only hydrogenase active site is mainly discussed in this thesis.

因此，唯铁氢化酶活性中心的化学模拟是本论文的主要研究内容。

The activity of H2 production of Fe-only hydrogenase is 10-100 times as high as that of hydrogenase.

其中，唯铁氢化酶的产氢活性是镍铁氢化酶的10-100倍。

By the role of hydrogenase, hydrogen with biohydrogen-bacterium produced can quickly reach the balance of hydrogen isotope exchange with water medium.

在氢化酶作用下，产氢菌形成的氢气与水介质之间可以迅速的达到氢同位素交换平衡。

The way of the formation of methane through CO2/H2 reduced, the main origin of hydrogen elements are from production of anaerobic degradation of organic matter in the role of the hydrogenase with biohydrogen-bacterium.

对于CO2（H2）还原途径形成的甲烷，氢元素主要来源于产氢菌在氢化酶的作用对有机质的厌氧降解。

The recent growing up of chemistry of hydrogenases, especially the Fe-only hydrogenase have renewed interest in classical organometallic diiron dithiolate complexes.

近几年发展起来的氢化酶化学，尤其是唯铁氢化酶活性中心的化学模拟研究，把铁硫簇化学的研究推向了又一高潮。

Types of hydrogenase are reviewed,i ts applications have found its appli cations,such as biohydrogen production,wastewater treatment,prevention of microbial-induced corrosion,generation and regeneration of NADP cofactors.

介绍了氢化酶的类型，讨论了氢化酶在生物制氢、废水处理、预防微生物腐蚀、NADP辅因子的产生及其再生等生物技术领域中的应用。

Since the first crystal structure of the Fe-only hydrogenase was published in 1998, intensive efforts have been spent on the structural and functional models of Fe-only hydrogenase due to its great capability of catalyzing the reduction of protons to molecular hydrogen at extraordinarily high rates.

自从1998年唯铁氢化酶的第一个晶体结构发表以来，由于它可高效地催化质子还原成氢气，其活性中心的结构和功能模拟受到了广泛关注。

Also, some enzymes are named according to the type of reaction they catalyze, such as phosphorylase or dehydrogenases.

此外，某些酶是根据它们所催化的反应类型来命名的，例如磷酸化酶或脱氢化酶。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程，我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理，建立了一种简捷的比色法，用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂，在495 nm和615 nm处检测二者的光吸收值的变化，可以测定酶活。本方法的优点是，可在比较宽的酶浓度范围内进行活力测定，而且操作简单、节省样品、灵敏度高。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。