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Normal controls received normal saline both intratracheally and orally.

对照组气管内和灌胃均用生理盐水。

Methods:PPPS was instilled intratracheally. The control group was given saline only.

将PPPS水化后,经气管滴入治疗整肺灌洗型成年兔呼吸窘迫模型,观察兔的生命体征、血气、动态肺顺应性等指标的变化。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red.

除N组外,其余各组采用气管内注入BLM致大鼠肺纤维化模型,分别于3、7、14、28、56 d处死各组大鼠,右肺行苏木精-依红、Masson胶原及天狼猩红染色,测定左肺羟脯氨酸的含量。

All the animals received conventional mechanical ventilation, but in liquid ventilation group a volume of 30 ml/kg of perfluorocarbon was given intratracheally after lung lavage was completed.

所有动物行常规机械通气,但液体通气组在模型制成后立即经气管插管给予全氟化碳液体,剂量30 ml/kg,在3小时内测定血气及肺顺应性。

METHODS: Except normal control group, the remaining 5 groups of rats were exposed to tobacco smoke and received a single intratracheally instillation of porcine pancreatic elastase to induce emphysema models.

除正常对照组外,其余5组大鼠均采用烟熏加气管内一次性滴注猪胰弹性蛋白酶法建立肺气肿模型。

To establish the pulmonary fibrosis model, the last four groups received BLM intratracheally at a dose of 3 mg/kg, NS group received NS by the same way.

后4组按3 mg/kg,气管内注入BLM建立肺纤维化模型,NS组采取相同手术方式,注入等量生理盐水。

Methods By intratracheally instillation, two strains of mice (C57BL/6 and C3H/He mice) were exposed to PM2.5 once time per day, for continuously two days.

采用气管滴注的染毒方法,两种品系的小鼠(C57BL/6小鼠和C3H/He小鼠)均予PM2.5染毒,连续染毒2d,最后一次染毒24h后,处死小鼠,取肺组织,并且立即置于液氮中保存。

The SHR and STZ-diabetic rats were intratracheally administered with PM2.5 and PM10-2.5 of different seasons.

我们利用气管灌注方式来进行暴露。

The Sprague-Dawley rats were intratracheally instilled with lipopolysaccharide(LPS,2 mg·kg-1 per day) for two days to induce acute lung injury.The rats were sacrificed at 72 hours after LPS instillation.Lung morphology was studied.

气管内注入LPS复制大鼠急性肺损伤模型,观察72 h后的病理变化、支气管肺泡灌洗液中细胞总数的变化、免疫组织化学特点以及蓝桉油的影响。

Methods Forty C57BL/6 mice (female, 8 wk of age, 17 to 20 g body weight) were randomly divided into two groups (20 mice in each group): in the group V, bleomycin was injected intravenously once in a dose of 150 mg/kg, and in the group I, bleomycin was instilled intratracheally in a dose of 5 mg/kg, respectively.

8周龄雌性C57BL/6小鼠40只,随机分为静脉注射组20只、气管内滴入组20只,分别经尾静脉一次性注射BLM150 mg/kg和气管内滴入BLM5 mg/kg。

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