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Methods Genomic DNA was extracted from 50 strains of S.schenckii, including 49 clinical isolates and 1 referrence strain, as well as from 1 strain of Mucor, Aspergillus fumigatus and Candida albicans, respectively, as controls. Four primers were designed according to previous studies, and screened by PCR.

以50株不同地区来源的孢子丝菌临床分离株及标准株的基因组DNA作为研究样本,以毛霉、烟曲霉、念珠菌临床分离菌株基因组DNA作为对照,通过特异引物PCR扩增的方法筛选国内外文献中报道的4对孢子丝菌引物。

Donor bacteria, through its pilus bacteria with receptors contact, the former transmission of different length single strand of DNA to the latter, and in the latter cells for double-stranded or further nuclear chromosome in exchange, integration, so that the latter was the donor of the genetic traits phenomenon.

供体菌通过其性菌毛与受体菌相接触,前者传递不同长度的单链DNA给后者,并在后者细胞中进行双链化或进一步与核染色体发生交换、整合,从而使后者获得供体菌的遗传性状的现象。

Tritici with MICs as 80 and 150μg/ml . The MICs of a main secondary metabolite of YS 4108, 3-methylphenol ether against Candida albicans, Tricophyton rubrum, Epidermophyton floccosum, Cunninghaghmella elegans and Aspergillus niger were 10, 30, 20, 20 and 50μg/ml, respectively.

来源于海洋真菌YS 4108的一个甲基酚醚表现出对白色念珠菌、红色毛癣菌、絮状表皮癣菌、雅致小克银汉菌和黑曲霉等五株人类病原真菌的良好抗菌活性,MIC分别为10、30、20、20和50μg/ml。

Trametes trogii was screened from Majia Hill in Weihai. The effect of pH, temperature and inorganic ion on Trametes trogii xylanase activity and stability was studied. Meanwhile, the substrate concentration effect of this xylanase was also studied and Km was measured.

从威海市玛珈山上获得毛栓菌,在对其产酶条件研究的基础上,主要研究pH、温度、无机离子对毛栓菌木聚糖酶活性和稳定性的影响,以及该酶底物浓度效应和Km值测定。

Methods Three primers, including4,5 and M13 core sequence (5'-GAGGGTG~GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes.

方法采用寡核苷酸重复序列4,5及M13中心序列(5'-GAGGGTGGCGGTTCT-3')等3种引物,扩增 20株红色毛癣菌和须癣毛癣菌临床分离株的DNA,观察产物电泳带型的差异。

When the concentration of NaCl was further increased to 8%, the cells of P. chrysosporium were deformed and plasma was completely vacuolated, indicative of a severe damage of P. chrysosporium. The DNA similarity was reduced to blow 67% and the rate of abnormal cells was increased to about 90%. The cells of P. chrysosporium thus essentially lost their original structure and function.

chrysosporium的结构与功能产生不良影响;NaCl浓度≥8%可对黄孢原毛平革菌产生严重损伤,细胞变形严重,空泡化,DNA相似度降至67%以下,异常细胞量约90%,表明此浓度范围可使黄孢原毛平革菌基本丧失了原有的结构与功能。

The biodegrading effect of 2,4-Dichlorophenol from aqueous solution by immobilized Phanerochaete chrysosporium was studied and the feasibility of using immobilized Phanerochaete chrysosporium to treat the chlorophenols-contamination water was investigated.

研究固定化黄孢原毛平革菌对水溶液中2, 4-二氯酚(2, 4-DCP)的降解效果,探讨固定化黄孢原毛平革菌处理水溶液中氯酚类污染物的可行性。

Biosynthesis and assembly of K88 fimbriae are regulated by the fae operon,which contains 10 genes designated faeA-faeJ.

菌毛的合成与装配是由fae操纵子调控的,fae操纵子包含10个基faeA~faeJ,其中有些基因表达菌毛装配所需的各种结构蛋白、分子伴侣和调控因子。

Slide culture: The strains were cultured on SDA,PDA,BCP-MSG, Milk Honey Agar and Vitamin B Complex Agars at 27℃and 30℃ for 3 weeks.

结论SDA、PDA均为鉴定和分离红色毛癣菌的合适培养基。5种培养基均可用来刺激红色毛癣菌产孢,其中SDA、PDA产孢较早、较丰富。

The fimbriae are the important virulence factors in enterotoxigenic E. coli. To detect and identify the four main fimbriae genes (K88, K99, F41, 987p) of ETEC from diarrhoeal piglets, a multiplex PCR assay was developed.

为了对猪源产肠毒素性大肠杆菌的4种主要菌毛(K88、K99、F41和987p)进行快速检测和分型,建立了检测4种菌毛的多重PCR方法。

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Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象,运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查,调查结果发现,石家庄市初中生物教师这一群体普遍存在职业倦怠,情感枯竭程度偏高,成就感偏低,去个性化程度最为严重。

In measurements of a day,generallyspeaking,the photosynthesis of birch in mesophytic habitat is better than that in xerophytichabitat(peak values are 12.8,10.33μmolCO2m-2s-1 respectively);that of sexual birch inmesophytic habitat is better than that of clone birch(peak values are 9.87,6.71μmolCO2m-2s-1respectively);that of young tree is better than that of seedling(peak values are12.37,10.05μmolCO2m-2s-1 respectively).

在一天中的各个时刻,总体说来,中生生境生长的白桦光合作用超过旱生生境生长的白桦光合作用(净光合速率峰值分别为12.8、10.33μmolCO2m-2s-1);白桦幼树的光合作用超过白桦幼苗(净光合速率峰值分别为12.37、10.05μmolCO2m-2s-1);中生生境有性白桦的光合作用超过无性白桦的光合作用(净光合速率峰值分别为9.87μmolCO2m-2s-1、6.71μmolCO2m-2s-1)。