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We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

Shrubs or trees, monoecious or dioecious; indumentum of simple hairs and gland-tipped hairs or sessile peltate-stellate hairs with central cells.

灌木或乔木,雌雄同株或异株;单毛和顶端有腺体的毛,或具中央细胞的无柄盾形星状毛被。

From the tests,we have obtained some structural variant features related with salt resistance and demonstrated the successful introduction of Rhizophora apicularta DNA into tomato.This thesis contains two parts. In the first part,the morphological and anatomical structures of the leaves were investigated. As to the morphology,some difference between control and transformed tomato were found. The leaf dimensions of the transformed tomato with unextended leaves were smaller than that of control. These structure changes should help to decrease the evaporation of water effectively and release the physiological drought resulted from saline.

本实验分两部分:第一部分、对转化番茄的形态及细胞显微结构进行观察与分析,实验表明转化番茄形态与对照相比有明显的变化,其叶面积变小,叶形收拢,这种结构可有效地减少水分的蒸发,缓解由盐胁产生的生理干旱;组织解剖结构结果显示,耐盐番茄在细胞结构上发生了明显的变异:其气孔器倾向下陷,表皮毛增多,叶脉微管组织不发达,微管束周围出现增多的贮水细胞,叶缘及其它部位表面分化出大型的多细胞分泌型结构,这些结构与转化番茄后代较强的耐盐能力有关。

In the studies on transferring and identifying alien chro-mosomes into wheat,the combined use of mitotic and meioticchromosome N-banding is also successfully practiced in deter-mination of T.durum-H.villosa amphipoid,five wheatalien disomic additions with H.villosa chromosomes,〓〓,alien sulstitution line with chromosome 〓 andsome translocation lines.

在外源染色体导入、鉴定研究中,利用根尖有丝分裂中期染色体和花粉母细胞减数分裂中期Ⅰ染色体的N-分带,快速、准确地鉴定了硬粒小麦一簇毛麦双二倍体、普通小麦一簇毛麦异耐加系V〓、V〓、V〓、V〓和V〓,普通小麦一簇毛麦异代换系V〓以及一些具有易位的杂种后代。

The peltate hairs were composed of one base cell, one stalk cell and a head of eight to twelve secreting-cells.

多细胞盾状腺毛由1个基细胞、1个柄细胞和一个由8-12个分泌细胞构成的头部组成。

Results Cuticular papillates,glandular hairs,peltate scales,and non-glandular hairs presented on the leaf epidermis of C.paliurus.Anomocytic stomata were confined to the lower epidermis.Large cluster crystals existed in the uppermost layer of palisade cells.Midrib included one closed vascular system and one additional vascular strand.Secondary veins are semicraspedodromous and form marginal loops,tertiary veins are percurrent,quaternary veins form orthogonal or polygonal areoles filled up by branched and freely ended veinlets.

结果青钱柳叶表皮有许多小乳突,腺毛,盾状鳞片和非腺毛表皮毛;气孔器不规则型,仅分布于下表面;靠近表皮的叶肉组织中有规律地分布含簇晶的细胞;主脉中维管组织环状,封闭,具有一个副维管束;二级脉在叶缘内分支,相互连接形成脉环,三级脉及顶型,脉间区内充满分支的自由结束的小细脉。

About a year after pollination, the generative cell of the four-celled male gametophyte undergoes division, giving rise to two kinds of cells—a sterile cell and a spermatogenous cell.

在授粉后大约一年,四个中生殖细胞-细胞男性的配偶体接受区分,引起细胞的二个类型-一个不毛的细胞和一个 spermatogenous 细胞。

ANP and CX43 began to express at 2nd week after induction and increased gradually,about 60% of the resulting myogenic cells were positive at 4th week after induction ,they were negative for uninduced cells.hMSCs'surface antigen profiles obtained by Flow Cytometry were negative for CD31\CD34\CD45 before and after induction,but CD90 expressed higher after induction while was weak positive before induction(P.05). Apotosis index was correlated with the cultural time after induction,The apoptosis rate of induced hMSCs was remarkably higher than control group(P.01),and the variation between groups was notable(P.05),the cell cycle analysis showed that the percentages of G_0/G_1phases were reduced significantly after induction. The expresstion levels ofβ-MHC and CTNT mRNA were undetectable before induction,began to increase at 1st、4th week after induction,reached the peak at 6th week and decreased after that,the expression of Bcl-2 and Bax mRNA varied regularly after treated with 5-azacytidine. hMSCs'resting membrane potential、range and rate of depolarization were heightened gradually after being induced.

结果:hMSCs诱导前为纺锤形,诱导后第2天部分细胞即开始发生形变,呈球形或短棒状,1周后胞浆中颗粒增多,约20~30%细胞边缘呈毛刷样变化;hMSCs表面抗原CD31、CD34、CD45在诱导前后差异无统计学意义,CD90未诱导时表达呈弱阳性,诱导后明显增高(P.05);ANP和CX43在诱导前无表达,诱导后第2周开始表达且表达随时间逐渐增强,但CX43在诱导后第5周表达量开始降低。hMSCs诱导后凋亡指数随诱导后培养时间增加,低浓度诱导组低于标准浓度诱导组,组间差异有统计学意义(P.05),G_0/G_1期细胞比例诱导后较对照组显著减少(P.05);β-MHC和CTNT基因分别在诱导后第1周和第4周时表达开始增强,在第6周时均达到高峰,第8周时表达开始衰减,Bcl-2、Bax基因表达呈时间依赖性变化,hMSCs经诱导后随心肌样细胞特征的表达膜静息电位、去极化幅值和去极化速率逐渐增高。

There were many acidophil corpuscles in hepatic sinus.

Subacute severe hepatitis 1 肝细胞水肿,肝细胞亚大块坏死,纤维组织紊乱,肝窦内见许多嗜酸性小体,肝细胞及毛细胆管淤胆明显,肝细胞部分脂肪变性,汇管区充血,有炎症细胞浸润

There were many acidophil corpuscles in hepatic sinus.

Subacute severe hepatitis 1 肝细胞水肿,肝细胞亚大块坏死,纤维组织紊乱,肝窦内见许多嗜酸性小体,肝细胞及毛细胆管淤胆明显,肝细胞部分脂肪变性,汇管区充血,有炎症颈椎病细胞浸润

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。