毛细胞

Objective To further understand the biological features of the human vestibular organ.

目的 进一步了解离体人前庭毛细胞的胞内钙离子（Ca2+）活动。

Methods Using the long-distance RT-PCR and a mammalian expression system, the full length cDNA of the α1D was cloned from cochleae and then expressed in HEK293 cell line.

以耳蜗基底膜中组织总RNA为模板，利用长距离RT-PCR克隆耳蜗毛细胞表达的电压门控钙通道α1D剪切异构体，并利用异源表达系统在哺乳动物细胞系进行表达。

The inner ear expressed a tissue-specific splicing isoform of α1D, and that the cell line constantly expressed this isoform can be used in physiologic and pharmacologic researches of L-type voltage-gated Ca2+ channel expressed in cochleae.

内耳毛细胞表达组织特异性的α1D剪切异构体，该异构体在外源表达系统中的表达为今后研究耳蜗α1D L-型钙通道蛋白的电生理和药理特性奠定了基础。

The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

分离解剖耳蜗后，用碘化丙啶染色细胞核、pholloidin染色f-actin，免疫荧光抗体分别染色单链dna、核酸内切酶g和凋亡诱导因子（apoptosis inducing factors， aif），制备耳蜗铺片，激光共聚焦显微镜下观察凋亡和坏死毛细胞内的荧光信号变化。

Methods Fifty F-344 rats were divided into four groups, GM only、PAA+GM、PAA only and saline control. ABR thresholds at different frequencies were measured at different times and hair cell losses were numerated. Two-dimensional diffusion assays in the culture medium were performed to evaluate the effect of PAA on the antimicrobial activity.

选用健康F-344大鼠50只，随机分4组：Ⅰ为GM、Ⅱ为PAA+GM、Ⅲ为PAA、Ⅳ为生理盐水对照组；通过观测4组大鼠不同时期、不同频率听性脑干反应（auditory brainstem respons，ABR）阈值的改变；计数耳蜗毛细胞死亡率，以观察PAA对F-344大鼠GM耳蜗毒性的拮抗作用；用双向扩散血清培养基检测法观察PAA 对 GM 抗菌活性的影响。

Otoacoustic emission is a release of audio-frequency energy from the outer hair cells of the inner ear into...

耳声发射是由内耳中外毛细胞产生，经听骨链、鼓膜传导，释放人外耳道的音频能量。

Methods Fifty white guinea pigs with normal hearing were randomly divided into the control group, ligustrazine group and traditional Chinese drug group. The alternations of hearing function were evaluated by the examination of auditory brainstem response. Some specimens were perfused by AgNO3 and observed the damage of bristle cell. And some were sent for immunohistochemistry to detect the expression of iNOS. Results Compared with the control group, the threshold of ABR in ligustrazine group and TCD group were tignificantly different.

将50只健康白色红目豚鼠随机分为对照组、顺铂组和中药组，各组动物均于用药前及停药后测试听觉脑干诱发电位，采用耳蜗铺片观察毛细胞损伤情况，应用免疫组织化学方法测定各组动物耳蜗内iNOS的表达情况。

Methods Establishing a model of whole cerebral ischemia-reperfusion in rats; examining the expressions of TrkA and NGF on cochleas assuming the method of immunohistochemistry stain; and examining the time and distribution of the cochlear hair cell apoptosis through the terminal deoxynucleotidyl transferasemediated dUTP nick end labeling.

建立大鼠全脑缺血再灌注模型，采用免疫组织化学染色方法检测TrkA和NGF在耳蜗中的表达；用TUNEL法检测耳蜗毛细胞发生凋亡的时相和分布。

This promoter is shown to direct the specific expression of the reporter gene,B-glucuronidase, in trichomes of Arabidopsis.

将此启动子与gus报告基因相连构建植物表达载体并转基因拟南芥，GUS组织化学定位分析表明此启动子在拟南芥叶片和茎的表皮毛细胞中特异表达。

It has two large otoliths which vibrate with the sound and stimulate surrounding hair cells.

它的二大otoliths以声振动和刺激周围的毛细胞。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失