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The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法,包括细胞作为微反应器的衍生法,借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

Using progesterone and bovine serum albumin as model compounds respectively, fluorescent labeled small molecule hapten and large molecule antigen were prepared.

1以孕酮和牛血清白蛋白为模型化合物,制备了小分子半抗原和大分子抗原的荧光标记物,研究了标记物的抗原活性和光谱性质,结果表明标记物是有效的毛细管电泳免疫分析试剂;制备了抗体F〓片段,研究了其抗体活性,结果表明F〓片段比整个抗体更适合作为毛细管电泳的免疫分析试剂。

It was found that well-defined separation of EP was achieved with decoupling devices containing on-line pretreated the working electrode, and the working electrode located very close to the capillary outlet (10-20 μm).

采用自行组装的毛细管电泳柱端安培检测系统,以肾上腺素为分离检测对象,系统考察了工作电极与毛细管末端对齐的距离、去耦器和工作电极的预处理等对分离检测的影响。

According to the mass transfer model of pure condensable gas under the capillary condensation mechanism, an accurate model improved by the factor of concentrations was put forward to calculate the permeability of components for binary gas mixtures.

以纯组分气体的毛细管冷凝传质模型为基础,提出了具有组分浓度修正项的二元混合气体的毛细管冷凝传质模型,模型能够准确地估算各组分的渗透系数。

The appearance of capillary electrophoresis is a revolution in traditional electrophoretic technique.

毛细管电泳的出现是对传统电泳技术的一次革命,它结合了电泳的高效分离原理和色谱的毛细管柱制备方法与自动化操作技术,成为当。。。

A pCEC instrument to study the separation behavior of solutes on 320μm CEC column was established, and the separation of neutral solutes with low concentration organic salt as the mobile phase was realized. Furthermore, nine compounds with similar structure were separated in a saltless system.

采用搭建的加压毛细管电色谱仪探讨了溶质在320μm内径毛细管柱上的分离行为,并且在低浓度的有机盐溶液作流动相条件下实现了中性溶质的分离。

A pCEC instrument to study the separation behavior of solutes on 320 um CEC column was established, and the separation of neutral solutes with low concentration organic salt as the mobile phase was realized. Furthermore, nine compounds with similar structure were separated in a saltless system.

采用搭建的加压毛细管电色谱仪探讨了溶质在320 μm内径毛细管柱上的分离行为,并且在低浓度的有机盐溶液作流动相条件下实现了中性溶质的分离。

The history of CE was retrospected and the basic theory of CE was introduced briefly.

对毛细管电泳的发展历史做了简单的回顾,同时对毛细管电泳的一些基本概念和原理做了简单的介绍。

In Chapter 6, Five kinds of different pesticides, such as simazine, atrazine, carbaryl, ametryne and prometryn were separated by CE technique and the effects of experiment parameters including pH, the concentration of surfactant and organic modifier on the separation were investigated.

采用毛细管电泳法对5种农药(西玛津、阿特拉津、扑草净、莠灭津和西维因)进行了分离研究,考察了溶液的pH值、胶束浓度和有机改性剂对分离的影响,添加阴离子表面活性剂的胶束毛细管电泳法可使各组分达到基线分离。

In this work, a microfluidic capillary electrophoresis system coupled to a flow injection sample introduction system using short capillary column as separation channel is described.

采用较短的毛细管作为分离通道,建立了一种微流控毛细管电泳和流动注射联用的分离测定麻黄碱和伪麻黄碱的新体系。

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