毛滴虫

The regulatory elements of the tetracycline resistance operon of Eschenchia coli, Tet repressor and Tet operator, have served as the basis for the establishment of heterologous repression-based inducible system in many eukaryotes including yeast, plants, insects, mammals and parasitic protozoa, such as Trypanosomes, Entamoeba, Giardia, Toxoplasma, Leishmania and Trichomonas.

基于四环素抑制子(Tetracycline Repressor，TetR)和四环素操纵子(Tet Operator，TetO)相互作用的四环素可诱导转基因表达系统，已经广泛的应用于很多真核生物，包括酵母、植物、昆虫、哺乳类细胞和诸如锥虫、阿米巴、贾第虫、弓形虫、利什曼和毛滴虫的寄生原虫。

Objective To clone and characterize a Racl homologue from Trichomonas vaginalis for studying cell cycle of the organism.

目的获得阴道毛滴虫Rac1蛋白的cDNA克隆，研究其在细胞周期中的调解作用。

Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （ Pf ., Tg ., Tv .） and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点，与华支睾吸虫LDH同源性最高为75％，与阴道毛滴虫LDH同源性最低为17%，与人LDH（HsLDH-A，-B，-C）的同源性为58%~60％； SjLDH与果蝇的进化距离较秀丽隐杆线虫为近，3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近；该蛋白具有3个跨膜区域，3个高亲水性区域，主要的线性表位98aa~106aa位于膜外，与原虫LDH相同区域差异显著，而与其他LDH有1~3个氨基酸的差异，关键催化位点之一及底物丙酮酸结合区域位于该区域，蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构，3个关键催化位点位于该区域或在其附近。

Objective: To search the characteristic proteins of Trichomonas vaginali and explore the influence of Pulsatilla chinensis on discrepancy protein expression.

目的：寻找阴道毛滴虫的特异蛋白质并探讨白头翁对其蛋白质差异表达的影响。

NBT and safranin O were used to stain the sample. The interaction between PMNs and the parasites was observed under microscope.

用氮蓝四唑和沙黄O染色，显微镜观察中性粒细胞与阴道毛滴虫相互作用及甲臢（NBT还原产物颗粒沉积。

Results The infecting rate of trichomonad was 3.55%; the infecting rate of candida albicans was 6.87%; the infecting rate of chlamydia trachomatis was 1.72%; the infecting rate of gonorrhea was 0.45% the infecting rate of syphilis was 0.11%; the infecting rate of ureaplasma urealticum was 13.49%, no positive for HIV antibody.

结果 毛滴虫的感染率为3.55%；白色念珠菌的感染率为6.87%；沙眼衣原体的感染率为1.72%；淋球菌的感染率为0.45%，梅毒螺旋体的感染率为0.11%；解脲支原体的感染率为13.49%；人类免疫缺陷病毒抗体未检出。

Trichomoniasis is a sexually transmitted infection that affects both men and women and results in roughly 7.4 million new cases in the United States each year, according to the U.S.

毛滴虫病是一种可在男性和女性间传播的性传染疾病，根据美国疾控中心的数据，美国每年有大约七百四十万新发病患者。

Both men and women with trichomoniasis hae an increased susceptibility to HI infection and may transmit HI to their sexual partners.

患有毛滴虫病的男性和女性患者都更易受HI病毒感染，也更易在它们的性伙伴中传播HI。

Gallinae remarkably; Anticandine, Ciprofloxacin and Complex Sulfamethoxazole had partial inhibition effect; Tylosin, Complex Licorice and Berberine Hydrochloride had little effect.

结果表明，二甲硝唑、替硝唑具有显著的杀毛滴虫作用，制霉菌素、环丙沙星、复方磺胺甲唑仅有部分抑制作用而不能将滴虫完全杀灭，泰乐菌素、复方甘草、盐酸小檗碱杀虫作用不明显。

METHODS:The minimum inhibitory concentration and the minimum bactericidal concentration of tinidazole lotion were m easured using tube culture test in vitro.In vivo effect of tinidazole lotion on trichomonas vaginalis were studied with the model of trichomonas vaginalis in ra bbits.

采用肉汤试管稀释法测定抗厌氧菌的MIC及MBC；采用家兔阴道感染人阴道滴虫的模型，观察其抗阴道毛滴虫的作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。