毛原细胞

AIM Effects of strophathidin on intracellular calcium concentration were investigated in isolated ventricular myocytes of guinea-pig.

目的：观察毒毛旋花子苷原对分离豚鼠心室肌细胞内游离钙离子浓度的影响。

Chromosomal analysis of skin cells from 8 tufted deer were studied by using primary culture and subculture.We found 4 different karyotypes in all and a new karyotype, different with the others studied before, was found. There were 47 chromosomes in somatic cell of the new karyotype, including a pair of heteromorphic X chromosomes, one was telocentric chromosome, the other was subtelocentric chromosome.

采用原代培养和传代培养方法对来自成都动物园的8头毛冠鹿的皮肤细胞进行了染色体研究，结果出现4种不同的核型，有一种核型与以前所报道的几种核型不一致，确定为新核型（2n=47♀，X染色体为异型，）。

Both the new peniculus 1 and the old haplokinety separated at the telophase.The two germinal rows germinal rowsfor both the daughter cells appeared almost at the same time;(2) The macronucleus became shorter and thicker, finally oblate during the division of the cell. Then the macronucleus restored its original shape, band-like form. The micronucleus were divided into two before the macronucleus division;(3) The original scopula and stalk contributed to the old cell. The new cell formed scopula gradually after the cell division.

同时，新仔虫的第一咽膜(P′1)也开始由老单毛基索复制，并在细胞分裂后期与老结构分离；(2)大核在虫体分裂过程中由长带状逐渐缩短变粗至扁圆形，于虫体即将分开时迅速拉长，然后分裂为二个新大核；小核分裂先于大核，在两仔虫口纤毛器即将分开时完成；(3)原帚胚及柄归属老仔虫，新仔虫的帚胚在虫体分裂后逐渐形成，柄内肌丝则在柄鞘形成后逐渐长出。

Primary culture and subculture were adopted using lung cells of a female Elaphodus cephalophus' embryo. A new karyotype and a pair of big telocentric chromosomes were found. The diploid has 48 chromosomes, and the karyotype formula is 2M+2ST+42T+XX. C-banding analysis shows that these chromosomes are homologous with those reported relevant chromosomes. This article is the first to report the polymorphism of Elophodus cephalophus B-chromosomes and studies it's transmitted mechanism.

采用原代培养和传代培养法，对1头毛冠鹿的胚肺组织细胞进行观察，发现一种新的核型，其二倍体染色体数目为2n=48，核型公式为2M+2ST+42T+XX，出现1对大的末端着丝粒染色体，C-带显示该染色体与已报道的相关染色体同源，首次提出毛冠鹿B染色体多态，对其传递机制进行了探讨。

The plasmid of pEGFP-N1 was preserved in our laboratory.②The rat vibrissa follicles were dissected under a stereomicroscope. The dermal sheath was moved by incubated in dispase. The bulge regions of the hair follicles in anagen phase were carefully cut between the arrector pili muscle and the sebaceous gland, and then incubated with a mixture of trypsin and EDTA. The cell suspension was selected, and cultured in 10% fetal bovine serum DMEM/F12 FAD medium. After 7 days culture, HFSC was obtained by rapid adhering on collagen Ⅳ.

实验方法：大鼠在体视显微镜下分离出含真皮鞘的完整毛囊，dispase消化，将毛囊从真皮鞘中挤出，收集形态完好且处于生长期的毛囊，分别在毛球部上端、皮脂腺下端横切毛囊，取中间部分置于胰酶和EDTA中联合消化，向所得细胞悬液中添加含10%胎牛血清DMEM/F12完全FAD培养基，常规培养7 d后，采用IV型胶原快速贴壁法两次筛选以分离纯化大鼠毛囊干细胞。

The results showed that ZBC can improve the rats mental state, diet, hair, increase their body weight,decrease remarkably ALT, AST, and GGT activity, raise ALB content and A/G, decrease the levelof serum HA, LN, PC III and IV-C, and decrease Hyp content of liver tissue, restrain mRNA expression of III collagen, relieve remarkably the inflammalion damage, fatly degeneration of the liver cells, and reduce the collagen fibre of the liver.

枳鳖胶囊可明显改善肝纤维化大鼠的精神状态、饮食、毛色泽，增加其体重；显著降低血清AST、ALT、GGT，显著升高血清ALB、A／G；降低血清HA、LN、PCⅢ、Ⅳ-C和肝组织Hyp含量；明显减轻大鼠肝细胞损害、肝脏脂肪变性和胶原纤维增生的程度；抑制Ⅲ型胶原mRNA的表达。

Result The highest level of growth factor 61.1μg/L,was produced by trichophyton rubrum(turbidimetry 4.5×10 10 ),and the strongest effect was at the point of double diluted filtrarate,cell index reached 2800 cpm/dis...

结果 红色毛癣菌分泌EFG最高 6 1 。1 μg/L(浊度比 4 。5× 1 0 10 )，对倍稀释液对培养的原代人类角质形成细胞作用最强，角质形成细胞标记指数可达 2 80 0cpm/盘。

Types of reactors for water treatment were established by Phanerochaete chrysosporium with Biebrich Scarlet as a substrate and cell immobilization.

以染料比佈列希猩红为降解对象，採用细胞固定化技术，建立6种黄孢原毛平革菌的水处理反应器工作体系。

I have therefore generated several p130cas RNA interference transgenic lines to determine the possible function of p130cas during neurogenesis. Knockdown of p130cas expression in developing neurons resulted in partial fusion in the commissures axon fascicles of CNS, photoreceptor axon targeting and guidance defects, and bulged synapse boutons at the neuromuscular junction. Moreover, reduced expression of p130cas in sensory organ precursor cells and proneural clusters resulted in bristle development defects.

为了了解p130cas的功能以及更进一步的分析p130cas在神经发育过程所扮演的角色，我建立一系列可以执行RNA干扰技术的转殖果蝇，结果显示在果蝇的神经中降低p130cas的表现会造成腹部神经索中原来排列整齐的commissure axons神经束偶尔会出现不正常的融合；若将p130cas专一地在果蝇眼睛中降低则会造成果蝇视神经突触目标辨识与突触导向的缺陷；而如果局部的运动神经系统中降低p130cas的表现会造成在神经肌肉接点的神经突触有澎大的现象；此外在果蝇的感觉器官前细胞以及原神经细胞丛，降低p130cas的表现则会导致刚毛发育的缺陷。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失