比高

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

Dielectric, piezoelectric and electrmechanical coupling properties of PZT and PSZT systems at high Zr content region were studied. The feature of high Kt and low Kp of PZT-based materials near F-AF phase boundary was exploited and examined, and electromachanical coupling anisotropy Kt/Kp is higher than that at the morphotropic phase boundary , Kt≥0.40, Kt/Kp≥3. 0. This phenomenon can be realized by electric field -induced AF-F phase transition and explained by coupling effect of two-sublattice in antiferroelectric unit cell.

研究了PZT和PSZT两个系统在高锆区的介电、压电和机电耦合等性能，首次发现了PZT基材料在铁电-反铁电相界处具有高Kt和低Kp的性质，机电耦合各向异性Kt/Kp也比准同型相界要高，Kt≥0.40，Kt/Kp≥3.0，并把这一现象归因于电场诱导AF-F相变和反铁电双子晶格间的强耦合作用。

Reinhardtii; by 13梸20% or 12?20% in S. obliquus; by 35挆81% or 29~79% in C. pyrenoidosa, respectively. On the other hand, specific growth rate in low or high P media, when CO2 concentration increased from 10 to 51 and 99~tM, increased by 13-46% or 10-44% in C. reinhardtii; by 15梸20% or 8-42% in S.

此外，当CO_2从10μM增加到51和99μM时，莱因衣藻的比生长速率在低P条件下增加13～16％，在高P条件下增加10～14％；斜生栅藻的，在低P条件下增加15～20％，在高P条件下增加8～12％；蛋白核小球藻的，在低P条件下增加20～44％，在高P条件下增加26～111％。

Consumable mainly are: Italy Biesse high quality 0.38 and 0.55mm types; British Cheshire high quality ceramic anilox roller and chrome anilox rollers; Doctor blades from Swiss, Sweden, Germany and Janpan; Australia Rotometrics diecutting cylinders, magnetic cylinders, flexible die and hot foil cylinders; Recyl waterbased and UV general cleaning solution, Cobra anilox roller cleaning materials, steel brush from France ; Screeny from Swiss and US; High quality printing cylinders with harden gear; idle cylinders; cooling cylinders; ink rollers and so on.. Related equipment mainly are: Alphasonics ultrasonics cleaning machine; Non-stop splicer, rewinder and sheet delivery; Plate mounter;Offline Printing quality inspection machine with slitting faction; cold foil stamping; hot foil stamping unit with saver and hologram function; web gravure unit; GEW UV curing machine; spare parts and UV lamp.

耗材主要有：意大利BIESSE比艾斯高质量双面胶；英国CHESHIRE高质量网纹辊；瑞士，德国和日本刮墨刀；澳大利亚ROTOMETRICS模切辊，磁性辊，磁性刀皮和烫金辊；法国RECYL水性，UV通用清洗液，COBRA网纹辊清洗膏，钢丝刷；进口丝网；英国超声波清洗机用浓缩清洗液；高精度印版辊带有精磨齿轮，导纸辊，水冷辊；配套设备主要有：英国ALPHASONICS超声波网纹辊清洗机；不停机放卷，不停机收卷和模内标收集台；装版机；分切复卷和质量检测设备；冷烫金装置，带有印刷定位烫功能的热烫金装置；凹印单元； GEW UV固化设备，零配件，UV灯管。

SLon vertical ring pulsating high gradient magnetic separator s,which has the advantages of large concentration ratio, high separation efficiency, strong adaptability, uneasy-to-be blocked magnetic medium and high availability, has found large-scale application in domestic mines in recent years.

SLon立环脉动高梯度磁选机具有富集比大、选矿效率高、适应性强、磁介质不易堵塞、设备作业率高的优点，近几年在国内的矿山已经大规模应用。

objective to evaluate efficacy of extracorporeal shock wave lithotripsyfor treating ureteral stones in situ,investigate the cause of higher re-treatment rate.methods total of 687 patients with ureteral stone were received eswl between january 2000 and december 2004,included 455 male(66.2%) and 232 female(33.8%) patients,6 cases have bilateral ureteral calculi,12 cases have unilateral multiple calculi.hence,together 709 ureteral calculi were treated.patients upper ureteral calculi were treated in the supine position,for lower ureteral calculi patients were turned prone.to reduce eswl-induced renal trauma and pain,using lower energy source,adjusted power setting from 9.8 to 13.2kv,limited 1500 shock wavs per one session.no auxiliary procedure were used before eswl.the stone size was measured as the surface area of stone length by stone width on x-ray film.the interval between two treatment sessions was two weeks.results of 709 ureteral calculi,the overall stone free rate was 97.3%(690 calculi),re-treatment rate was 34.1%(292 calculi).according to the performed treatment sessions,one session 467 calculi,the mean stone size 37.27mm2,stone free rate 65.4%(464 calculi).two sessions 138 calculi,the mean stone size 62.48mm2,stone free rate 18.4%(131calculi).three sessions 52 calculi,the mean stone size 79.60mm2,stone free rate 7.1%(50calculi).four sessions 19 calculi,the mean stone size 101.63mm2,stone free rate 2.4%(17calculi).fivesessions 33 calculi,the mean stone size 119.33mm2,stone free rate 3.9%(28 calculi).overall 19 cases(2.7%)turned to other treatment modalities.of 335 upper ureteral calculi,303 achieved stone free (95.8%),re-treatment rate was 38.5%(129 calculi).of 374 lower ureteral calculi,369 achieved stone free(98.7%),re-treatment rate was 30.2%(113 calculi).the re-treatment rate of upper ureteral calculi was higher than lower ureteral calculi(p＜0.05,χ2=5.40).the difference of stone-free rate between upper and lower ureteral calculi was no significant(p＞0.05,χ2=0.15).conclusion eswl should be considered first line therapy for ureteral stone still.stone burden are the main variable of higher re-treatment rate,upper ureteral stone may moving with respiring during eswl.so efficinet shock wave was decreared,re-treatment rate become higher.

目的 评估体外震波碎石治疗输尿管结石的疗效，探讨再治疗率高的原因及输尿管结石的治疗选择。方法回顾2000年1月～2004年12月间eswl治疗输尿管结石的临床资料687例，男455例（66.2%），女232例（33.8%），平均年龄46.6岁（15～83岁）。有双侧输尿管结石6例，单侧多发性输尿管结石12例（4颗1例，3颗2例，2颗9例），共计输尿管结石709颗（含透光结石13颗）。应用上海爱申公司生产的desunit6030型碎石机，c臂x线球管做结石定位。上段输尿管结石（肾盂输尿管交界处至骶髂关节上缘）取仰卧位，下段输尿管结石（骶髂关节上缘下至输尿管口）取俯卧位。为减少eswl引起的肾损伤和疼痛，应用较低的能量，震波发生器电压从9.8～13.2kv，震波频率1.5s。每次治疗设定为1500次震波。治疗后3天摄腹部平片或b超，以后每隔7日重复检查。假如结石未碎或有残留结石最长径＞3mm以上，再次eswl，两次治疗的间隔时间为两周。结石的大小用x线片上的表面积（mm2表示。结果 709颗输尿管结石总的治愈率为97.3%（690颗），再治疗率34.1%（242颗）。其中一次治疗467颗，平均结石大小37.27mm2，治愈464颗（65.4%），3颗改治疗；两次治疗138颗，平均结石大小62.48mm2，治愈131颗（18.5%），7颗改治疗；第1和第2次治疗治愈率（1个月治愈率）为83.8%。3次治疗52颗，平均结石大小79.60mm2，治愈50颗（7.1%），2颗改治疗；4次治疗19颗，平均结石大小101.63mm2，治愈17颗（2.4%），2颗改治疗；5次及5次以上治疗33颗，平均结石大小119.33mm2，治愈28颗（3.9%），5颗改治疗。总计19颗（2.7%）结石改变治疗方式。上段输尿管结石335颗，治愈321颗（95.8%），再治疗129颗（38.5%）。下段输尿管结石374颗，治愈369颗（98.7%），再治疗113颗（30.2%）。经χ2检验，上、下段输尿管结石的再治疗率差异有显著性（χ2=5.40，p＜0.05），治愈率差异无显著性（χ2=0.15，p＞0.05）。不良反应：血压升高13例（1.9%），震波区域疼痛26例（3.8%），震波进入处皮肤点状淤血33例（4.8%），肉眼血尿128例（18.6%），均于第2、3天自行消失。结论 eswl目前仍是输尿管结石的第一线治疗，结石的大小是再治疗率高的主要因素。结石的位置有影响，上段输尿管结石可随呼吸移动，有效震波次数减少，再治疗率比下段输尿管结石高。eswl前注重病例筛选可降低再治疗率。

The results confirm that NH3 slip increases with both higher ratio of NH3 to NO n(NH3/n and higher concentration of a reducing reagent, while it decreases with the increase of temperature. Initial NO concentration and its residence time can also affect the NH3 slip. A higher initial NO concentration lead to higher NH3 slip, but added residence time has the opposite effect. Among urea, ammonium carbonate and ammonia liquor, the NH3 slip of urea is the highest and that of ammonia liquor is the lowest.

试验结果表明：温度越高，漏失氨含量越低；随氨氮物质的量比的增加，NH3漏失含量不断增加；还原剂质量浓度越低，NH3漏失含量越低； NO初始浓度和停留时间对漏失氨浓度也有影响，NO初始浓度越高NH3漏失浓度越高，停留时间越长漏失氨浓度越低；不同还原剂NH3漏失浓度不同，尿素、碳酸氨、氨水3种还原剂中，尿素的NH3漏失浓度最高，氨水的NH3漏失浓度最低。

Organically reared cows, which eat high levels of fresh grass, clover pasture and grass clover silage, produced milk which is on average 50% higher in vitamin E, 75% higher in beta carotene (which our bodies convert to Vitamin A) and two to three times higher in the antioxidants lutein and zeaxanthine than non-organic milk.

有机饲养的奶牛，吃的高水平的新鲜草，苜蓿牧草和青贮草三叶草，生产牛奶，这是对平均高50 ％，在维生素E， 75 ％为高，在β胡萝卜素（即我们的身体转换成维生素A ）和两高出三倍，在抗氧化剂叶黄素和zeaxanthine比非有机牛奶。

Mean daily consumption of alcohol per day was greater in men than in women (28.2 25.0 g vs 13.9 15.0 g), as reflected in both in the lowest (mean, 4.1 g vs 1.1 g) and highest (mean, 68.7 g vs 38.8 g) quintiles of daily consumption. More than 50% of women consumed less than 10 g a day of alcohol, and more women were abstainers than men (3.0% vs 2.1%).

男性的平均酒精日摄取量要比女性高28.2 ( 25.0 g vs 13.9 ( 15.0 g)；最低(平均， 4.1g vs 1.1g)及最高(平均， 68.7g vs 38.8g)的日摄取量也是男性高於女性，超过10%的女性，日摄取量低於10 g；对酒精节制的女性人数也高於男性(3.0% vs 2.1%)。

For frequency information fluid identification technology:the basic theory of fluid identification based on frequency information has been introduced,for inner and external factor affecting frequency,some steps have been proposed to attain reserved amplitude and frequency analysis data with high resolution,high S/N ratio;through the comparison of time-frequency analysis method, the high accuracy time-frequency analysis method has been selected to extract time-frequency attributes;the analysis method flow of common single frequency fluid identification has been proposed,according to the data of full frequency band,several anomaly zone modes of common single frequency attribute have segregated; incorporating converted wave and compression wave,fluid identification method based on PS wave combined time-frequency analysis has been proposed;the AVO property of various fluid type in frequency domain has been studied;the generalized fourier analysis method,Proni absorption filter has been introduced,and researched for anti-noise and for the seismic processing interpretation flow.The above technology have been applied to fluid identification of organic reef in WBT area in the east of Sichuan and fluid identification of clastic rock in GUANGAN area in the middle of Sichuan,the results show that the above methods are effective.

基于频率信息的流体识别技术方面：介绍了利用频率信息流体识别的基本理论，针对影响频率的内、外在因素，提出一些针对措施，以获得高分辨率、高信噪比的保幅、保频分析数据；通过时频分析方法的比较，选取高精度的时频分析方法提取时频属性；提出共单频属性进行流体识别的分析方法流程，结合全频带数据，分出几种共单频属性异常带模式；联合转换波资料和纵波资料，提出基于纵横波联合时频分析进行流体识别方法；研究了频率域内不同流体类型AVO现象；引进一种广义傅立叶分析方法：Proni吸收滤波，研究其抗噪性以及进行地震处理解释的流程；将这些技术应用川东北五百梯区块进行生物礁储层流体的识别和川中广安陆相碎屑岩的流体识别，取得较好的效果。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。