比色的

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

Linen also comes in varied quality and weight, has a finer and smoother texture than cotton, and unprimed linen is light brown in color. I prefer the surface of linen for painting, however, it is more expensive. If you plan to do more than a few paintings, it is usually better to buy a larger piece of canvas, since less will be wasted with a larger piece when cutting for various canvas sizes. You can find fabric canvas, and panels and pre-stretched canvases in art stores, and some office supply stores and department stores carry canvas panels and pre-stretched canvases.

床单也丰富多彩的质量和重量，有一个更精细和平滑纹理比棉， unprimed亚麻是浅棕色的颜色，我喜欢表面上的麻布画，却是更昂贵的，如果您打算做多过几个画，它通常不如买一个大尺寸的油画，自少被浪费了一个更大的片切割时，由于种种画布大小，你可以找到面料帆布，和小组预拉伸画布在美术商店，及一些办公用品商店及百货公司进行帆布板和预拉伸画布。

Concentrations of water soluble phenolic substances in the leaves of male and female trees of Acer negundo L.were successively determined within an entire growing season by method of iron tartrate colorimetric analysis.

采用酒石酸铁比色法对复叶槭雌、雄株叶片中水溶性酚类物质的含量进行了一个生长周期的跟踪测定，对不同性别、不同年龄、不同光照条件下的复叶槭植株叶片中水溶性酚类物质的含量做了比较分析，同时研究了叶片中叶绿素的含量与水溶性酚类物质含量之间的关系。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

objective to investigate the inhibition mechanism of nanoapatite on the human hepatocelluar carcinoma.methods stable and single-dispersed nanoapatite were synthesized with sol-gel method.nanoapatite were surveyed by zataplus and tem.their effect on the bel-7402human hepatoma cell lines was investigated by the mtt methods,and the mechanism was studied from changes in ultrastructure.results the result showed that inhibition of nanoapatite on the bel-7402human hepatocellular carcinoma cell lines was presended obviously in vitro.conclusion nanoapatite entered into cancer cytoplasm,and cancer cells result in oncosis.

目的 探讨纳米磷灰石的抑癌机制。方法利用sol-gel法制备出稳定单分散的纳米磷灰石体系，应用粒径电位仪和透射电镜对纳米磷灰石进行表征；采用mtt比色法研究纳米磷灰石对细胞系bel-7402人肝癌细胞的作用；从超微结构的改变研究其作用机制。结果实验结果表明，纳米磷灰石在体外对bel-7402人肝癌细胞具有明显的抑制作用。结论通过进入到癌细胞内，导致癌细胞胀亡。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

The samples of decalcified bone matrix, composite material and recombinant human bone morphogenetic protein 2 were respectively co-incubated with C2C12 cells for 72 hours.

细胞裂解后用比色法分别检测裂解液中成骨细胞特异性标记物碱性磷酸酶及总蛋白的吸光度值，以两者的比值表示单位数量的C2C12细胞所含有的碱性磷酸酶含量，以此衡量待测生物材料成骨活性的高低。

Relation equation between blackbody temperature and two color gray ratio of thermal radiation image was set up.

3进行激光熔池测温专用软件需求和可行性分析，描述了激光熔池热辐射图像的数据流程，采用模块化思想进行软件设计，基于数字图像处理技术，开发出一套激光再制造熔池测温专用软件，详细介绍了热辐射图像的数据采集、输入/输出、图像处理、温度计算模块及程序设计开发。4介绍了常用黑体辐射源的结构、加热控温方式和性能评价方式，对不同类型的黑体辐射源进行了详细描述，选择中国计量科学研究院的标准高温黑体辐射源进行温度标定，建立了热辐射图像比色值与温度之间的对应关系。

During this years, TCM has a prominent effect on Dysfunctional Dyspepsia,so we study the effect of SiNiSan of soup on the Dysfunctional Dyspepsia sharply from the ability of stomach emptying and the ability of intestines pussing on the normal Kunming rats by the coloring matter,and the chang of the nerve fiber, nerve qualitative, on the stomach organize of normal SD ratsMethods:In the experiment, the thirty Kunming rats, whose avoirdupois are about twenty grammes,and thirty S. D rats, whose avoirdupois are about two hundred grammes,were randomly divided into three groups, 0. 9yS group, SiNiSan of soup group and Mosapride Citrate group.

本课题通过对正常小鼠胃内葡聚糖蓝-2000残留量、小肠内葡聚糖蓝-2000推进率，来研究四逆散对小鼠胃排空、小肠推进的影响；通过采用正常大鼠肠道气囊法，记录正常大鼠用药后肠收缩幅度和频率的变化；采用医学彩色图象全自动分析系统，分析用药后正常大鼠胃窦组织中NOS阳性神经元分布面积和酶活性的变化；采用硝酸酶还原法和比色法，检测用药后正常大鼠胃窦组织中NO和TchE的含量变化，通过以上几个方面，研究四逆散的促胃肠动力作用及其作用机制。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。